FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005486978

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486978
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17240737
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGCT19300.084.869456
ACGACGC21150.079.158375
CACGACG23900.070.353444
GACGCTC29100.056.5362327
TACACGA34800.049.782092
CTACACG37800.044.6480671
ACGCTCT37600.043.9480638
ACACGAC45850.038.0954363
CGCTCTT50150.033.3836489
CGTTGAG26750.07.8864341
CGCCAAT15158.48231E-67.4597631
TAACCCT575550.06.51040176
GTTCCGA35651.8189894E-126.5055755
CGTGATG48450.06.3758271
CTAACCC622750.06.2380135
CCTAACC638400.06.0510344
CGTCAAG18801.7063014E-46.0114581
CCCTAAC648750.05.97684053
ACCCTAA643300.05.9719582
CGATGAG26806.530736E-75.90379051
Produced by FastQC (version 0.11.5)