FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005486985

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486985
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15455973
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGCG10200.086.704729
CGGTACG21650.076.573011
TTTGCGC26950.076.353061
TGCGCGA24150.076.1731951
CGCCGAA26000.075.2276841
GTGCGAC25550.073.137651
ACGACCG23200.071.770551
GACGCGT22700.070.78891
GCGCTAG27900.068.80161
TTACCCG33200.067.6735841
CGAACGC28150.067.157371
GCGCGTA25250.065.9436341
GCCGGAT32000.065.894141
GTACCGT33950.065.771488
CCGCTAT30000.064.7125851
TCGCGAT16500.064.149848
GTCGGAC22750.063.601951
ATAACGC27650.063.3753971
TGACGGC33950.063.1802021
CGCGGTA30450.062.8010941
Produced by FastQC (version 0.11.5)