FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005486991

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486991
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18116909
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGCT27050.092.641416
ACGACGC28350.089.415195
CACGACG30700.082.3347854
GACGCTC37800.065.720167
TACACGA41050.062.686622
CTACACG41950.052.9979931
ACGCTCT47600.052.950328
ACACGAC53300.047.9652823
CGCTCTT62950.040.03889
CGATGGA30500.010.4134231
CGTGATG51450.09.9946511
CGCTGAG35750.08.0380671
CGTAGAG28950.07.8364031
CGGGTTA27350.07.74185041
CGTTGAG26151.8189894E-117.51875161
CGAGACG13108.2265564E-57.5044021
CGATGAG27951.2732926E-117.30509761
CGTAAAG23708.1545295E-97.0196981
GTACACG23304.3553882E-86.81565241
TAACCCT760350.06.486796
Produced by FastQC (version 0.11.5)