FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005486997

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486997
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17412307
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGCT49550.0115.2520146
ACGACGC49800.0115.246275
CACGACG52000.0110.649194
GACGCTC60050.094.73637
TACACGA65700.088.13912
CTACACG59500.082.7015841
ACGCTCT69750.081.457368
ACACGAC71400.081.0997543
CGCTCTT81200.070.3278669
GTACACG26250.014.088021
TCTTCCG108800.011.37619510-14
CTTCCGA118000.010.90684110-14
TTCCGAT139550.09.47181110-14
CGATCTA39500.09.09966915-19
ATACACG44000.08.9193641
TCCGATC141150.08.91271215-19
CGGGTTA24100.08.7684771
TCGCCGG22650.08.640923145
CCGATCT149450.08.62138215-19
CGCAAAG31800.07.1199561
Produced by FastQC (version 0.11.5)