FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005486999

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486999
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18407875
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGCT27650.0100.651616
ACGACGC30450.092.586345
CACGACG32900.086.1317144
GACGCTC40000.069.574857
TACACGA44600.063.862842
ACGCTCT47000.059.2128078
CTACACG45550.056.442371
ACACGAC53800.053.075613
CGCTCTT62800.044.6607979
CGCTGAG37950.08.7413841
CGGGTTA29300.07.9768851
CGTTGAG27454.802132E-106.86652331
CGATGGA32057.2759576E-126.8219611
TCTTCCG99950.06.74346110-14
CGGTTAG15851.2183728E-46.65944431
CGTAAAG25253.0568117E-86.5690191
GTACACG22501.2444234E-66.366641
GTTCCGA51000.06.2526935
CGCCAAT15808.4512413E-46.20333961
TAACCCT690250.06.1843426
Produced by FastQC (version 0.11.5)