FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005487000

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005487000
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17094924
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGCGA25950.081.285251
CGCCGAA27700.077.7308651
TTACGCG9900.076.877139
CGGTACG24700.076.829431
GCGCTAG31050.076.6314851
GTGCGAC30700.074.889931
TTTGCGC25900.074.3971
CGAACGC32850.066.877861
ATAACGC31850.066.686021
GGCGACT34600.066.659531
GCGCGTA30200.066.220861
GACGCGT27800.066.161751
TTACCCG31250.064.930091
CCTTACG32400.064.878191
ACGACCG26100.062.6410061
TCGACGG30650.062.6291471
TAGGCGC32100.062.528631
CCGCTAT32650.062.36951
GCGCAAC37300.061.638631
CCGATAA35450.060.94341
Produced by FastQC (version 0.11.5)