FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005487001

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005487001
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17094924
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGCT48600.0100.157666
ACGACGC50500.097.681155
CACGACG52700.093.191034
GACGCTC58350.083.171317
TACACGA68350.072.4464952
ACGCTCT69250.070.405338
CTACACG58850.070.338911
ACACGAC79550.062.5563773
CGCTCTT79200.061.194339
GTACACG30150.014.6216731
CTTCCGA107000.010.11509210-14
TCTTCCG105050.09.9718410-14
TTCCGAT124400.08.9332210-14
TCCGATC128200.08.74025415-19
CCGATCT134750.08.41221915-19
CGATCTA38550.07.40754815-19
TTACACG35850.07.08634231
ATACACG50000.06.72473051
GCGTCGG37250.06.6141586145
GCTCTTC181700.06.187850510-14
Produced by FastQC (version 0.11.5)