FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005488073

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005488073
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25025333
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG419000.046.5879454
CGTATGC418950.046.42643455
GCTTGAA408050.046.21510770
TATGCCG418650.046.14206357
CTCGTAT425550.045.92020853
TCTCGTA425700.044.76135652
ATGCCGT428300.044.65304658
ATCTCGT433600.043.85695651
CCGTCTT432500.043.54783661
TGCCGTC441200.043.1967659
GCCGTCT440600.042.9537660
CGTCTTC461900.040.8972362
CACTCGT525950.039.90331631
ACTCGTC527250.039.77830532
GTATGCC492450.039.5468656
AATCTCG172900.039.2658450
TCACTCG535750.039.21259330
TATCTCG136200.038.69510750
CTCGTCT545500.038.46682433
TCGTCTC551100.038.00555834
Produced by FastQC (version 0.11.5)