FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005488074

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005488074
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25025333
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAACGCACAGTGTAGATCT2530061.0109995339522555Illumina Single End PCR Primer 1 (96% over 33bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAACGCACAGTGTAGATC900640.35989131493275234Illumina Single End PCR Primer 1 (97% over 34bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGT450450.043.42838711
GCCGTAT460900.043.1654859
CGTCGTG452550.043.16499312
TAACGCA464350.043.1100132
AACGCAC469100.042.9492733
TCGCCGT463250.042.9478857
CGTATCA465250.042.82360561
GTAACGC469600.042.762231
TCGTGTA454700.042.5529914
AGCGTCG466350.042.36018410
CGCCGTA473800.042.353558
TGGTCGC475250.041.91549754
CCGTATC475700.041.8149960
GTCGCCG481650.041.77270556
GTCGTGT465400.041.74760413
TGTAACG474500.041.49231730
GTGTAAC484900.040.56606729
ACGCACA496900.040.50437534
AGAGCGT491200.040.304758
GGTCGCC499300.040.18391855