FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005488075

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005488075
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19647227
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG52850.039.79584554
CGTATGC52800.039.30330355
CTCGTAT53150.038.912853
TATGCCG56250.036.89270457
TCTCGTA57000.035.73192652
ATCTCGT62100.033.07901851
TCGCATT67350.032.42365334
ATGCCGT64900.032.08341258
CGCATTG70050.031.3242735
ACTCGCA70550.031.20116232
TCACTCG71250.031.1403730
CACTCGC72950.030.55860331
ACACGTC77600.028.95190813
CTCGCAT76600.028.73713133
TGCCGTC74400.028.22192459
CCGTCTT76300.026.96876761
ACGTCTG86250.026.37291315
GCCGTCT79050.026.20765360
CACGTCT90700.025.34906614
CACACGT102600.021.86324112
Produced by FastQC (version 0.11.5)