FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005488086

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005488086
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24617450
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTACAACAGCGTGTAGATCT1693750.6880282076332033Illumina Single End PCR Primer 1 (97% over 35bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTACAACAGCGTGTAGATC1203010.48868180904196007Illumina Single End PCR Primer 1 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATCGG277650.037.2190971
CGTATCA385150.035.42822361
TCGCCGT385900.035.42328357
GCCGTAT388500.035.16662659
TGGTCGC390750.035.00187754
CGCCGTA396650.034.67499558
GTCGCCG397350.034.5790356
GCGTCGT385550.034.35556411
GCGTGTA396700.034.31774539
AACAGCG398750.033.91284635
AGCGTGT402700.033.6481538
CGTCGTG395250.033.53013612
AGCGTCG399350.033.5274510
ACAGCGT405000.033.28580536
CCGTATC410200.033.2549560
TCGTGTA393800.033.23592814
AGTGTAC407150.033.02253328
GGTCGCC418500.032.9318455
GTCGTGT397950.032.90691813
GTGGTCG417750.032.8394353
Produced by FastQC (version 0.11.5)