FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005488087

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005488087
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22914363
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT238200.047.6885453
TCGTATG240300.047.63586454
CGTATGC240200.047.53914355
TATGCCG244450.046.6696857
TCTCGTA236600.046.5467452
ATCTCGT244950.045.0170851
ATGCCGT256700.044.29259558
CCGTCTT264600.042.58654461
TGCCGTC274100.041.37865459
GCCGTCT274900.041.05455460
CGTCTTC287750.039.22118862
GTCACAA301750.039.1888429
GCTTGAA290050.039.15737570
ACACGTC308450.038.4497213
ACGTCTG316500.037.56022315
CACGTCT319650.037.0587114
TATCTCG77500.036.16905250
GTATGCC318200.036.13887856
CACACGT334100.035.47685212
TGCTTGA324150.035.01649569
Produced by FastQC (version 0.11.5)