FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005488088

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005488088
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22914363
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTGAAGTGTAGATCT1518920.6628680884561357Illumina Single End PCR Primer 1 (97% over 34bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCCGT229300.050.15199357
TGGTCGC229650.050.1064754
CGTATCA232900.049.2260861
GCCGTAT233750.049.1050359
CGCCGTA235150.048.87455458
GTCGCCG237550.048.5137856
CCGTATC252400.045.42087660
CGTCGTG260500.044.3586112
GCGTCGT261400.044.31297711
GGTCGCC261800.044.11362555
GTGGTCG262450.044.0307553
GTAGATC266900.043.3790943
TCGGTGG270200.042.85878850
GATCTCG270600.042.70778746
GTATCAT270650.042.47802462
TCGTGTA274450.042.00189614
CGGTGGT276650.041.8848551
GAGTGTA273500.041.79143527
TCTCGGT281750.041.10040748
AGAGTGT278100.041.0875926
Produced by FastQC (version 0.11.5)