FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005488092

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005488092
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19974843
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTACAGAGCGTGTAGATCT2618661.31097901495396Illumina Single End PCR Primer 1 (96% over 32bp)
GTCTTGGTGGTGGGGAAGGACAGGAACATCCTCTCCAGGGCCTCCGCACC998840.5000489866178172No Hit
CTTTAATAGAAATTGGACAGCAAGAAAGCGAGCTTAGTGATACTTGTGGG872820.43695962967018065No Hit
GGTCGAAGTGCGGGAAGTAGGTCTTGGTGGTGGGGAAGGACAGGAACATC793920.397459944991808No Hit
GGCATGTCGTCCACGTGCGCCACGGCGTTGGTCAGCGCGTCGGCCACCTT752080.37651359762877734No Hit
CTCCAGCTTAACGGTATTTGGAGGTCAGCACGGTGCTCACAGAAGCCAGG722310.3616098509510187No Hit
CTCAGGTCGAAGTGCGGGAAGTAGGTCTTGGTGGTGGGGAAGGACAGGAA715510.3582055688748092No Hit
GTCGAAGTGCGGGAAGTAGGTCTTGGTGGTGGGGAAGGACAGGAACATCC667800.3343205250724624No Hit
CACCAACTTCATCCACGTTCACCTTGCCCCACAGGGCAGTAACGGCAGAC585360.2930486111955924No Hit
GTCAGCACGGTGCTCACAGAAGCCAGGAACTTGTCCAGGGAGGCGTGCAC569080.28489835940137304No Hit
CTCACCACCAACTTCATCCACGTTCACCTTGCCCCACAGGGCAGTAACGG559970.2803376226786864No Hit
GGTATTTGGAGGTCAGCACGGTGCTCACAGAAGCCAGGAACTTGTCCAGG542390.2715365522522505No Hit
GTCACAGTGCAGCTCACTCAGTGTGGCAAAGGTGCCCTTGAGGTTGTCCA541010.2708456832426668No Hit
CGGTATTTGGAGGTCAGCACGGTGCTCACAGAAGCCAGGAACTTGTCCAG533860.2672661807654758No Hit
GTCCAGGTGAGCCAGGCCATCACTAAAGGCACCGAGCACTTTCTTGCCAT517000.2588255637353445No Hit
ATCAGGAGTGGACAGATCCCCAAAGGACTCAAAGAACCTCTGGGTCCAAG511220.25593192397056636No Hit
ATTTGGAGGTCAGCACGGTGCTCACAGAAGCCAGGAACTTGTCCAGGGAG510300.2554713446308439No Hit
CTTAGGGTTGCCCATAACAGCATCAGGAGTGGACAGATCCCCAAAGGACT506040.25333866203604205No Hit
CTGGGCAGAGCCGTGGCTCAGGTCGAAGTGCGGGAAGTAGGTCTTGGTGG492630.24662521752986996No Hit
CAGCAAGAAAGCGAGCTTAGTGATACTTGTGGGCCAGGGCATTAGCCACA484450.24253006644407668No Hit
GCGGGAAGTAGGTCTTGGTGGTGGGGAAGGACAGGAACATCCTCTCCAGG478780.23969149594817843No Hit
GGGCATGTCGTCCACGTGCGCCACGGCGTTGGTCAGCGCGTCGGCCACCT471560.23607694939079119No Hit
GGGAGGAGGGCCCGTTGGGAGGCCCAGCGGGCAGGAGGAACGGCTACCGA462370.2314761622907374No Hit
GTTGGACTTAGGGAACAAAGGAACCTTTAATAGAAATTGGACAGCAAGAA461260.2309204633047679No Hit
GTCGTCCACGTGCGCCACGGCGTTGGTCAGCGCGTCGGCCACCTTCTTGC449900.2252333097186296No Hit
GTCCACGTGCGCCACGGCGTTGGTCAGCGCGTCGGCCACCTTCTTGCCGT446830.22369637648716437No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTACAGAGCGTGTAGATC444290.22242477700575666Illumina Single End PCR Primer 1 (96% over 33bp)
GGCAGAATCCAGATGCTCAAGGCCCTTCATAATATCCCCCAGTTTAGTAG438850.21970135134478905No Hit
GTCAGCGCGTCGGCCACCTTCTTGCCGTGGCCCTTAACCTGGGCAGAGCC438590.21957118761834574No Hit
GCTTGAAGTTGACCGGGTCCACCCGAAGCTTGTGCGCGTGCAGGTCGCTC428280.21440969523515152No Hit
CTTGGTGGTGGGGAAGGACAGGAACATCCTCTCCAGGGCCTCCGCACCAT427010.2137738954944477No Hit
GTCCAGGGAGGCGTGCACCGCAGGGGTGAACTCGGCGGGGAGGTGGGCGG424600.21256737787626168No Hit
CCCAGGGCCTCACCACCAACTTCATCCACGTTCACCTTGCCCCACAGGGC405760.20313551400629282No Hit
GTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTTGCC401420.20096278103412377No Hit
GTTGGGCATGTCGTCCACGTGCGCCACGGCGTTGGTCAGCGCGTCGGCCA397380.19894023697708163No Hit
GGCCGGTGCAAGGAGGGGAGGAGGGCCCGTTGGGAGGCCCAGCGGGCAGG373930.18720047011132954No Hit
CCTTAGGGTTGCCCATAACAGCATCAGGAGTGGACAGATCCCCAAAGGAC372180.18632436810642267No Hit
GGGAAGTAGGTCTTGGTGGTGGGGAAGGACAGGAACATCCTCTCCAGGGC365550.18300519308211835No Hit
GTGAACTCGGCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCT359080.17976611881254836No Hit
CTTGAAGTTGACCGGGTCCACCCGAAGCTTGTGCGCGTGCAGGTCGCTCA349970.17520538208986172No Hit
GACAGCAAGAAAGCGAGCTTAGTGATACTTGTGGGCCAGGGCATTAGCCA346600.17351825994326964No Hit
GTAGGTCTTGGTGGTGGGGAAGGACAGGAACATCCTCTCCAGGGCCTCCG344860.17264716423553367No Hit
CAGGAAGGGCCGGTGCAAGGAGGGGAGGAGGGCCCGTTGGGAGGCCCAGC333910.1671652688334021No Hit
CCAGGAAGGGCCGGTGCAAGGAGGGGAGGAGGGCCCGTTGGGAGGCCCAG330780.16559829781891153No Hit
CAGGGAGGCGTGCACCGCAGGGGTGAACTCGGCGGGGAGGTGGGCGGCCA326600.1635056656014768No Hit
CCTGCACTGGTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAG318690.15954568453929774No Hit
CTCGGCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGA309210.15479971482128796No Hit
CTTAGGAGCTTGAAGTTGACCGGGTCCACCCGAAGCTTGTGCGCGTGCAG307940.1541639150805841No Hit
CTTCACCTTAGGGTTGCCCATAACAGCATCAGGAGTGGACAGATCCCCAA302620.15150056498566722No Hit
CTGCACTGGTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGA293260.14681467083370817No Hit
GTGGCTTAGGAGCTTGAAGTTGACCGGGTCCACCCGAAGCTTGTGCGCGT282490.14142288878065276No Hit
GGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCTTGA280580.14046668602101153No Hit
GGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCTTGAA279270.13981086109162408No Hit
CTTGCCATGAGCCTTCACCTTAGGGTTGCCCATAACAGCATCAGGAGTGG274170.13725764953446692No Hit
GGACAGCAAGAAAGCGAGCTTAGTGATACTTGTGGGCCAGGGCATTAGCC268420.1343790286612015No Hit
GGGGAGGAGGGCCCGTTGGGAGGCCCAGCGGGCAGGAGGAACGGCTACCG266420.1333777692270222No Hit
GCACTTTCTTGCCATGAGCCTTCACCTTAGGGTTGCCCATAACAGCATCA266360.1333477314439968No Hit
GTGCAAGGAGGGGAGGAGGGCCCGTTGGGAGGCCCAGCGGGCAGGAGGAA264660.13249666092494444No Hit
GCAGAATCCAGATGCTCAAGGCCCTTCATAATATCCCCCAGTTTAGTAGT256790.12855670505144895No Hit
GCCTGCACTGGTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACA247160.12373564087587571No Hit
GCCCATAACAGCATCAGGAGTGGACAGATCCCCAAAGGACTCAAAGAACC231640.11596586766664449No Hit
GGGTGAACTCGGCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGG230150.11521992938818093No Hit
GGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTTGC229860.11507474677022493No Hit
GTGGGAAGTAGGTCTTGGTGGTGGGGAAGGACAGGAACATCCTCTCCAGG229760.11502468379851598No Hit
GGCCTCACCACCAACTTCATCCACGTTCACCTTGCCCCACAGGGCAGTAA229710.11499965231266149No Hit
CAGGAGTGGACAGATCCCCAAAGGACTCAAAGAACCTCTGGGTCCAAGGG227290.11378812839730455No Hit
CGGGAAGTAGGTCTTGGTGGTGGGGAAGGACAGGAACATCCTCTCCAGGG226300.11329250497738581No Hit
CTCTGGGTCCAAGGGTAGACCACCAGCAGCCTGCCCAGGGCCTCACCACC224430.11235632740642819No Hit
GTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCTTGAAGTTGA222990.11163542061381909No Hit
ACCAACTTCATCCACGTTCACCTTGCCCCACAGGGCAGTAACGGCAGACT222550.11141514353829965No Hit
CTTGTCACAGTGCAGCTCACTCAGTGTGGCAAAGGTGCCCTTGAGGTTGT218880.10957783247658066No Hit
CTCAGGATCCACGTGCAGCTTGTCACAGTGCAGCTCACTCAGTGTGGCAA214700.10748520025914597No Hit
CCTTGAGGTTGTCCAGGTGAGCCAGGCCATCACTAAAGGCACCGAGCACT209720.10499206426803956No Hit
CAGGAACTTGTCCAGGGAGGCGTGCACCGCAGGGGTGAACTCGGCGGGGA201950.10110217136625305No Hit
CATCAGGAGTGGACAGATCCCCAAAGGACTCAAAGAACCTCTGGGTCCAA201400.10082682502185374No Hit
CCTGGGCAGAGCCGTGGCTCAGGTCGAAGTGCGGGAAGTAGGTCTTGGTG200610.10043132754535293No Hit
CTTGAGGTTGTCCAGGTGAGCCAGGCCATCACTAAAGGCACCGAGCACTT200180.10021605676700437No Hit
CCAGGAACTTGTCCAGGGAGGCGTGCACCGCAGGGGTGAACTCGGCGGGG200080.10016599379529541No Hit
GCTTGTCACAGTGCAGCTCACTCAGTGTGGCAAAGGTGCCCTTGAGGTTG200080.10016599379529541No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA380000.054.1066261
GCCGTAT380200.054.0673359
TCGCCGT384150.053.87739657
TGGTCGC388250.053.48925854
CGCCGTA393150.052.92888658
CCGTATC389050.052.83728860
GTCGCCG397050.052.7356556
GCGTGTA386600.052.5769339
GTATCAT397900.051.57789262
GAGCGTG389200.051.52419337
AGCGTGT392750.051.3416938
GTGTTAC390000.051.25441429
GTGGTCG404600.051.1628853
GATCTCG400500.051.01687246
GTAGATC403700.050.80333343
CGTCGTG387100.050.71665612
AGTGTTA393450.050.6544128
GCGTCGT387850.050.6456511
GGTCGCC414150.050.41457455
AGAGTGT393550.050.2164726
Produced by FastQC (version 0.11.5)