FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005488093

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005488093
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25428783
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG302600.044.177854
CGTATGC302900.044.0069655
CTCGTAT304800.043.7785653
TATGCCG303950.043.65920657
TCTCGTA305900.042.23688552
ATGCCGT313600.041.96980358
ATCTCGT311700.041.43972851
GCTTGAA322500.040.56685370
TGCCGTC325550.040.27871759
GCCGTCT326600.039.988560
CGTCTTC342500.038.1627562
CGTCTTG370800.037.7518734
ACCGTCT379600.036.95095432
GTCACCG385450.036.62621329
ACACGTC387400.036.567113
ACGTCTG391400.036.24704415
CACCGTC388500.036.0504131
TCACCGT393800.035.6807530
CACGTCT401400.035.30915514
GTATGCC385450.034.71838456
Produced by FastQC (version 0.11.5)