FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005488096

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005488096
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23688076
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCACCTAGGTGTAGATC5435662.2946819319559766Illumina Single End PCR Primer 1 (96% over 33bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCACCTAGGTGTAGATCT2574821.0869688192489757Illumina Single End PCR Primer 1 (96% over 32bp)
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCACCTAGGTGTAGATCTC264770.11177353534326721Illumina Single End PCR Primer 1 (96% over 31bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATCGG759550.054.9239271
GCCGTAT1002400.042.32598560
TCGCCGT1006200.042.2584458
CGTATCA1004700.042.1946162
TGGTCGC1012900.041.99344355
GTCGCCG1021100.041.93654657
CGCCGTA1022850.041.7055659
CCGTATC1021650.041.51011761
ACCTAGG1010050.041.37703336
GCGTCGT1013000.041.1895912
TCACCTA1016950.041.14816734
CACCTAG1018250.041.07475735
CGTCGTG1017250.040.9624613
AGCGTCG1031300.040.76070411
GTATCAT1036600.040.737763
GTGGTCG1047400.040.59018354
GGTCGCC1053350.040.57021756
GATCGGA1033450.040.5306742
GTAGATC1053000.040.49985544
TCGGTGG1054500.040.46929651