FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005488097

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005488097
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21662058
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG218800.049.1980654
CTCGTAT219350.048.8832553
CGTATGC220650.048.75384555
TATGCCG223150.048.1444757
TCTCGTA218850.047.6037652
ATCTCGT223500.046.59768751
ATGCCGT239750.044.5189858
CCGTCTT250300.042.2230961
TGCCGTC261850.040.78832659
GCCGTCT262100.040.6559660
ACACGTC276150.040.6537213
GCTTGAA262450.040.26839470
GTGGTAC281850.040.06783735
CACGTCT287700.039.02163314
ACGTCTG288550.038.90668515
CGTCTTC273550.038.6599862
TGTGGTA293300.038.63507534
GATCGGA308100.037.16841
CACACGT305800.036.79149212
TATCTCG71250.036.73894550
Produced by FastQC (version 0.11.5)