FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005488099

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005488099
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23517674
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG152500.040.4799254
CTCGTAT152400.040.43759553
CGTATGC155550.039.68619555
TCTCGTA155500.038.82126252
TATGCCG160450.038.45224457
ATCTCGT165050.036.51140651
ATGCCGT171800.035.70811558
CCGTCTT180200.033.8493161
GCCGTCT187450.032.57745760
TGCCGTC193450.031.62138259
GCTTGAA204150.030.1182470
CGTCTTC206350.029.729362
ATCTACG220750.029.42327135
ACACGTC228600.028.59647413
ACGTCTG236650.027.60893615
CACGTCT238900.027.3342614
TATCTCG53100.026.88911850
TCTACGT69750.026.49130636
GTATGCC240200.026.02066656
CACACGT261550.025.14075712
Produced by FastQC (version 0.11.5)