FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005488100

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005488100
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23517674
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAAGCATCGGTGTAGATCT808040.34358840079167696Illumina Single End PCR Primer 1 (96% over 33bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAAGCATCGGTGTAGATC269470.11458190975859263Illumina Single End PCR Primer 1 (97% over 34bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA153900.040.47732561
TCGCCGT161600.038.63411757
GCCGTAT161750.038.6212459
TGGTCGC167250.037.5384754
CGCCGTA168150.037.3168258
GTCGCCG169750.036.96657656
CATCGGT167800.036.91410436
ATCGGTG169350.036.70015337
AGCATCG170200.036.6212334
CCGTATC179000.034.8798160
CGGTGTA181700.034.53272639
GCGTCGT181600.034.45509311
CGTCGTG183250.033.93479512
GTATCAT189950.033.14504262
GTGGTCG196650.032.24650653
GGTCGCC200800.031.667655
GTAGATC200900.031.56306343
TCGTGTA196300.031.46441814
GAGTGTA197150.031.36759827
GTCGTGT203500.030.35124813
Produced by FastQC (version 0.11.5)