FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005488101

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005488101
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23660107
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG268550.045.45266754
CGTATGC269800.045.20316755
CTCGTAT268800.045.0849253
TCTCGTA262050.044.88408752
TATGCCG274250.044.2526857
ATCTCGT270150.043.43468551
ATGCCGT285350.042.17543458
AGTCACG294850.042.04036728
CACGCTT298700.041.34575331
GTCACGC301750.040.92827629
GCTTGAA292150.040.9184470
ACGCTTC303650.040.740932
TCACGCT304500.040.5697130
TTCGAAC112350.039.9016236
TGCCGTC303850.039.6421259
ACACGTC322800.038.4538913
CCGTCTT309500.038.4208661
GCCGTCT315450.038.0067960
ACGTCTG331850.037.5001115
CGCTTCG330500.037.48402833
Produced by FastQC (version 0.11.5)