FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005488103

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005488103
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21977544
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATCGG1418850.042.723131
GCTTGAA544050.039.9834170
CGTAGGT1498500.039.35835
CCGTAGG1514650.039.04942734
ACCGTAG1520000.039.00407433
GTAGGTT1493700.038.95264436
CACCGTA1528650.038.71926532
GTCACCG1556450.038.0861630
TCGTATG1145800.037.92725855
CTCGTAT1162200.037.91599754
TCACCGT1565550.037.90724631
TCTCGTA1155300.037.89406253
CGTATGC1138150.037.87778556
TAGGTTA472450.037.8431837
TATGCCG1121050.037.80278858
ATCTCGT1160150.037.70849652
ATGCCGT1116200.037.39957459
TATCTCG295300.037.1283451
TAGGTTT489050.037.01662437
TGCCGTC1121150.036.93480760