FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005488105

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005488105
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20736629
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG455700.044.4560954
CGTATGC455200.044.43572655
CTCGTAT466550.044.0522953
TATGCCG458050.043.9224157
GCTTGAA440950.043.7130770
TCTCGTA456100.043.64215552
ATGCCGT461450.043.0224258
ATCTCGT463900.042.915951
CCGTCTT463050.041.87617561
TGCCGTC475000.041.64043859
GCCGTCT475100.041.13815760
TATCTCG137550.040.9866850
CGTCTTC484450.040.0046762
GTATGCC519150.039.0294556
TGCTTGA515600.037.58099769
GATCGGA671850.037.475211
CAGTCAC665800.037.17188627
AGTCACC667050.037.03927228
GTCTTCT529850.036.4712163
AATCTCG195400.036.37417650
Produced by FastQC (version 0.11.5)