FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005488108

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005488108
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25810206
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTAGGTTGGTGTAGATCT1036910.4017441782525874Illumina Single End PCR Primer 1 (96% over 32bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTAGGTTGGTGTAGATC281840.10919711373090166Illumina Single End PCR Primer 1 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA186350.042.81836761
TCGCCGT192050.041.63791357
CGCCGTA197200.040.51533558
TGGTCGC197200.040.4623354
GCCGTAT198900.040.15184859
GCGTCGT202250.039.60568211
GTGTCTA201300.038.75284229
GTCGCCG208450.038.46438256
CGTCGTG209000.038.17608312
CCGTATC213200.037.45880560
AGAGCGT219200.036.8463678
GTATCAT219350.036.5997362
TCTAGGT214600.036.4474732
TCGTGTA218800.036.25769814
CTAGGTT216200.036.162633
GAGTGTC217500.035.962227
GTGGTCG227100.035.36607453
GGTCGCC227700.035.2732255
GTCGTGT225950.035.20348413
GTAGATC229900.035.10163543