FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005488110

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005488110
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26919884
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCGTTAGAGTGTAGATCT1604630.5960761197930868Illumina Single End PCR Primer 1 (96% over 32bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCGTTAGAGTGTAGATC516070.19170587807882086Illumina Single End PCR Primer 1 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTTAG257700.047.53189533
CGTTAGA263000.046.62734234
TGCGTTA268050.045.72125632
CGCCGTA273150.045.43207658
CGTATCA273500.045.29775261
TGGTCGC279400.044.49053254
GTGCGTT279550.043.86526531
TCGCCGT284800.043.5241657
GCCGTAT288900.042.9316659
GTCGCCG292100.042.58170756
GTGTGCG293250.042.00879329
TCGTGTA291500.041.63281214
GGTCGCC301100.041.42407255
GCGTCGT299450.040.8899411
CCGTATC303900.040.77815660
TGTGCGT300900.040.73141530
GTGGTCG308800.040.44699553
GTCGTGT301950.040.2499913
TAGAGTG308050.040.07946837
CGTCGTG306400.039.8713712