FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005488111

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005488111
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28384182
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG700150.059.44396254
CGTATGC700500.059.3792955
CTCGTAT698300.059.3558853
TATGCCG702500.058.97102457
TCTCGTA687150.058.76062852
ATCTCGT698450.057.6897151
ATGCCGT714750.057.61749658
CCGTCTT720000.056.5362561
TGCCGTC733700.056.0148859
GCCGTCT735600.055.6560260
GCTTGAA736500.055.5880170
CGTCTTC749150.054.34105762
TATCTCG191900.053.55967350
ACACGTC807350.053.5106813
ACGTCTG816600.052.8145515
CACGTCT821200.052.5954114
GTATGCC794300.052.4596356
CACACGT844950.051.33160812
TGCTTGA803300.051.09617269
CGTCTGA848200.050.88405216
Produced by FastQC (version 0.11.5)