FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005488112

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005488112
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28384182
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCAATGGTGTAGATCT5627471.982607777810895Illumina Single End PCR Primer 1 (97% over 34bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCAATGGTGTAGATC460510.16224177254782257Illumina Single End PCR Primer 1 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA734150.057.8950961
TCGCCGT737000.057.6697957
GCCGTAT737100.057.66843859
TGGTCGC737750.057.56422854
CGCCGTA739400.057.5589358
GCGTCGT731950.057.42415611
CGTCGTG733000.057.11822512
GTCGCCG748050.056.89038556
TCGTGTA741000.056.19379814
CCGTATC761600.055.8270860
GTCGTGT748300.055.80957413
AGAGCGT765350.055.4849328
GTGGTCG769100.055.22176453
GGTCGCC771150.055.2166255
GAGTGTA755850.054.97896627
GTATCAT777450.054.73355562
GTAGATC776050.054.53561443
AGCGTCG781300.054.32087310
CGTGTAG769850.054.2470415
TAGGCAA774900.054.22614332