FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005488117

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005488117
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22041177
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACC260020.11797010658732064No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTTGAA1583900.063.3155170
CGTATGC1662000.062.8541655
TATGCCG1649550.062.84456657
TCGTATG1670200.062.8200554
CTCGTAT1677600.062.50539453
ATGCCGT1647200.062.35210458
CCGTCTT1632300.062.0957361
TCTCGTA1635950.062.0859652
ATCTCGT1633250.062.06217651
TGCCGTC1657900.061.84204559
GCCGTCT1652100.061.71162460
CGTCTTC1655600.061.16264762
GTATGCC1718950.060.50695856
TGCTTGA1679200.060.18897269
TATCTCG408400.059.9987950
CTGCTTG1711900.059.14134668
AATCTCG586750.059.05762550
GTCTTCT1726150.058.5087763
CATCTCG374450.058.0742450
CAGTCAC2003250.058.01988627
Produced by FastQC (version 0.11.5)