FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005488118

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005488118
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22041177
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGATGCTACGTGTAGATCT14031916.3662253608325905Illumina Single End PCR Primer 1 (96% over 32bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGATGCTACGTGTAGATC623160.28272537351340177Illumina Single End PCR Primer 1 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA1757500.062.3067051
GCGTCGT1702000.062.26156211
CGTCGTG1701000.062.01702512
TCGTGTA1693850.061.48008714
GTCGTGT1705900.061.3414813
AGCGTCG1747800.061.2907610
ATCGGAA1774150.061.2887462
TCGGAAG1781050.061.0999953
AGAGCGT1769900.061.0652128
AAGAGCG1802250.060.85847
CGGAAGA1799800.060.740844
GAGCGTC1771050.060.648179
GCCGTAT1871750.060.29890459
CGTATCA1871900.060.24117761
GCTACGT1832650.060.16217836
TCGCCGT1866950.060.13120757
TGCTACG1859650.060.09034335
CGCCGTA1885200.060.0122758
CCGTATC1884900.059.8559460
TGGTCGC1870650.059.83338554
Produced by FastQC (version 0.11.5)