FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005488294

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005488294
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7423794
Sequences flagged as poor quality0
Sequence length40
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTGTACTGAGCTGCCCCGAGAGATCGGAAGAGCACACG96477412.99570004232337No Hit
TCCTGTACTGAGCTGCCCCGAAGATCGGAAGAGCACACGT4969246.693666338263157No Hit
TCCTGTACTGAGCTGCCCCGAGAAGATCGGAAGAGCACAC4582476.172679360445616No Hit
TATTGCACTTGTCCCGGCCTGTAGATCGGAAGAGCACACG3518324.739247883225208No Hit
TCCTGTACTGAGCTGCCCCGAGTAGATCGGAAGAGCACAC3083604.153671289909176No Hit
AAACCGTTACCATTACTGAGTAGATCGGAAGAGCACACGT2316723.120668488376698No Hit
TCCTGTACTGAGCTGCCCCGAGATCGGAAGAGCACACGTC1880602.533206066870929No Hit
TATTGCACTTGTCCCGGCCTGTAAGATCGGAAGAGCACAC1856432.5006485901952558No Hit
TCCTGTACTGAGCTGCCCCGAAAGATCGGAAGAGCACACG1223511.648092606017893No Hit
CATTGCACTTGTCTCGGTCTGAAGATCGGAAGAGCACACG884241.191089084637855No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGA866871.1676913448837616No Hit
CAACGGAATCCCAAAAGCAGCTAGATCGGAAGAGCACACG720530.9705684182508297No Hit
TGAGGGGCAGAGAGCGAGACTAGATCGGAAGAGCACACGT585710.7889631635791619No Hit
TATTGCACTTGTCCCGGCCTGCAGATCGGAAGAGCACACG558210.7519201098521862No Hit
TATTGCACTTGTCCCGGCCTGTTAGATCGGAAGAGCACAC476910.6424073728338906No Hit
TCCTGTACTGAGCTGCCCCAAGATCGGAAGAGCACACGTC446440.6013636693044014No Hit
TCCTGTACTGAGCTGCCCCGAGCAGATCGGAAGAGCACAC437090.5887690310372298No Hit
TGTAAACATCCCCGACTGGAAGCTAGATCGGAAGAGCACA423650.5706650804157551No Hit
TGAGGTAGTAGTTTGTGCTGTTAGATCGGAAGAGCACACG384020.5172826724448443No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAGATCGGAA348400.4693018152173942No Hit
TATTGCACTTGTCCCGGCCTGAAGATCGGAAGAGCACACG308860.4160406390586808No Hit
AAACCGTTACCATTACTGAGCAGATCGGAAGAGCACACGT299330.40320353716711427No Hit
TATTGCACTTGTCCCGGCCTGAGATCGGAAGAGCACACGT280080.37727339955823125No Hit
TGGAGAGAAAGGCAGTTCCTGAAGATCGGAAGAGCACACG260330.35066975188158506No Hit
ATTGCACTTGTCCCGGCCTGTAGATCGGAAGAGCACACGT254390.3426684522765583No Hit
TTGAATTGAAGTGCTTGAATTAGATCGGAAGAGCACACGT250510.3374420141507159No Hit
TCTCTCGGCTCCTCGCGGCTCGAGATCGGAAGAGCACACG238030.3206312028593466No Hit
TGACATGGGACTGCCTAAGCTAAGATCGGAAGAGCACACG235080.3166574934595437No Hit
CATTGCACTTGTCTCGGTCTGAGATCGGAAGAGCACACGT202140.27228665019530446No Hit
AGCTCGGTCTGAGGCCCCTCAGTAGATCGGAAGAGCACAC188840.2543712823928035No Hit
TGAGGGGCAGAGAGCGAGACTTTAGATCGGAAGAGCACAC188650.2541153485670535No Hit
ACCACAGGGTAGAACCACGGAAGATCGGAAGAGCACACGT174810.2354725898913682No Hit
TCAGGCTCAGTCCCCTCCCGATAGATCGGAAGAGCACACG153670.20699658422634032No Hit
TCCTGTACTGAGCTGCCCCGAGGAGATCGGAAGAGCACAC152920.20598631912469553No Hit
CAACGGAATCCCAAAAGCAGCTGAGATCGGAAGAGCACAC150110.20220119254386637No Hit
CAACGGAATCCCACAAGCAGCTAGATCGGAAGAGCACACG149610.2015276824761032No Hit
AAACCGTTACCATTACTGAGTTAGATCGGAAGAGCACACG148790.20042312596497153No Hit
TCCTGTACTGAGCTGCCCCGTAGATCGGAAGAGCACACGT145550.19605878072586602No Hit
TCCTGTACTGAGCTGCCCCGATAGATCGGAAGAGCACACG145090.19543915146352392No Hit
TCCTGTACTGAGCTGCCCCTAGATCGGAAGAGCACACGTC135600.18265593037737848No Hit
TGAGGGGCAGAGAGCGAGACTTAGATCGGAAGAGCACACG129850.17491056459810173No Hit
TGAGGTAGTAGGTTGTGTGGTTAGATCGGAAGAGCACACG128570.17318637882462795No Hit
CGCGACCTCAGATCGGAAGAGCACACGTCTGAACTCCAGT128220.17271492177719372Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GGCTGGTCCGATGGTAGTGGGTTATCCGAACTAGATCGGA126840.1708560339901673No Hit
AGCTCGGTCTGAGGCCCCTCAAGATCGGAAGAGCACACGT126280.17010170271427252No Hit
AAAAGCTGGGTTGAGAGGGCGAAGATCGGAAGAGCACACG124070.1671247882147592No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACATGTCAATCTC123870.16685538418765392TruSeq Adapter, Index 15 (97% over 40bp)
CAACGGAATCCCAAAAGCAGCCAGATCGGAAGAGCACACG121530.16370335707052217No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACCAGATCGGA117820.15870591236771928No Hit
TCGCTCTGATACCAAATTGATGAGATCGGAAGAGCACACG113320.1526443217578505No Hit
TCAGGCTCAGTCCCCTCCCGAAGATCGGAAGAGCACACGT112330.15131077182367936No Hit
CCCCCCACTGCTAAATTTGACTGGCTTAGATCGGAAGAGC110380.14868408255940294No Hit
GATGGATATGTCTTCAAGGACAGATCGGAAGAGCACACGT110280.14854938054585026No Hit
TCCTGTACTGAGCGTCGCTAGATCGGAAGAGCACACGTCT108180.14572063826124487Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TACCACAGGGTAGAACCACGGAAGATCGGAAGAGCACACG106140.14297271718477103No Hit
TGTAAACATCCCCGACTGGAAAGATCGGAAGAGCACACGT105470.14207021369396833No Hit
TGTAAACATCCCCGACTGGAAGAGATCGGAAGAGCACACG96550.13005479408507292No Hit
AGCTCGGTCTGAGGCCCCTCAGATCGGAAGAGCACACGTC92590.12472059434838845No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATAGATCGGAA92080.12403361407926998No Hit
AAACCGTTACCATTACTGAGAGATCGGAAGAGCACACGTC91250.12291558736678308No Hit
AAGCTGCCAGTTGAAGAACTAGATCGGAAGAGCACACGTC89790.12094893796891454No Hit
AACCCGTAGATCCGAACTTGTAGATCGGAAGAGCACACGT87660.11807978508024335No Hit
TCCTGTACTGAGCTGCCCCCAGATCGGAAGAGCACACGTC87440.11778344065042753No Hit
TCCTGTACTGAGCTGCCCCGGAGATCGGAAGAGCACACGT85570.11526451299699318No Hit
TGAGGGGCAGAGAGCGAGACCAGATCGGAAGAGCACACGT85190.11475264534549315No Hit
TGTAAACATCCCCGACTGGAAGATCGGAAGAGCACACGTC83110.11195084346359825No Hit
TGAGGTAGTAGGTTGTGTGGTAGATCGGAAGAGCACACGT82470.11108875057686136No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCA81410.1096609092332034TruSeq Adapter, Index 15 (100% over 39bp)
AAGCTGCCAGTTGAAGAACTGTAGATCGGAAGAGCACACG81240.1094319158101639No Hit
CAAAGAATTCTCCTTTTGGGCTAGATCGGAAGAGCACACG77660.10460958372497944No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)