FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005488295

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005488295
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6256472
Sequences flagged as poor quality0
Sequence length40
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGA5496838.78583009721773No Hit
TCCTGTACTGAGCTGCCCCGAGAGATCGGAAGAGCACACG5051588.074167038548243No Hit
AAACCGTTACCATTACTGAGTAGATCGGAAGAGCACACGT4650817.43359836022602No Hit
TCCTGTACTGAGCTGCCCCGAAGATCGGAAGAGCACACGT3676095.875659636932763No Hit
TCCTGTACTGAGCTGCCCCGAGATCGGAAGAGCACACGTC1951833.1196974908542705No Hit
TCCTGTACTGAGCTGCCCCGAGAAGATCGGAAGAGCACAC1921173.070692236774975No Hit
TCCTGTACTGAGCTGCCCCGAGTAGATCGGAAGAGCACAC1308682.091721980055213No Hit
TCCTGTACTGAGCTGCCCCGAAAGATCGGAAGAGCACACG1229381.964973230919918No Hit
TATTGCACTTGTCCCGGCCTGTAAGATCGGAAGAGCACAC1195091.9101659849192962No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACCAGATCGGA1058301.6915283885231165No Hit
AAACCGTTACCATTACTGAGCAGATCGGAAGAGCACACGT882551.4106192755278053No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAAGATCGGA859331.3735057073699044No Hit
TATTGCACTTGTCCCGGCCTGTAGATCGGAAGAGCACACG729441.1658966906588888No Hit
TCCTGTACTGAGCTGCCCCAAGATCGGAAGAGCACACGTC630901.0083957859956856No Hit
TGAGGGGCAGAGAGCGAGACTAGATCGGAAGAGCACACGT540070.8632181203719924No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAGATCGGAA534130.8537239517734595No Hit
CATTGCACTTGTCTCGGTCTGAAGATCGGAAGAGCACACG482030.7704501834260586No Hit
TCGCTCTGATACCAAATTGATGAGATCGGAAGAGCACACG447220.7148117980868451No Hit
AAACCGTTACCATTACTGAGAAGATCGGAAGAGCACACGT318570.5091847290293955No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTAGATCGG308640.4933131643520502No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAGAG300210.4798391169975667TruSeq Adapter, Index 3 (97% over 37bp)
TCTCTCGGCTCCTCGCGGCTCGAGATCGGAAGAGCACACG289590.46286469435170496No Hit
TGAGGTAGTAGTTTGTGCTGTTAGATCGGAAGAGCACACG285870.45691885139100763No Hit
TCAGTGCACTACAGAACTTTGTAGATCGGAAGAGCACACG278820.445650519973557No Hit
GATGGATATGTCTTCAAGGACAGATCGGAAGAGCACACGT269020.42998674013085975No Hit
TTGAATTGAAGTGCTTGAATTAGATCGGAAGAGCACACGT258600.41333198646137953No Hit
CAACGGAATCCCAAAAGCAGCTAGATCGGAAGAGCACACG257860.41214921124876763No Hit
TCCTGTACTGAGCTGCCCCGAGCAGATCGGAAGAGCACAC251980.4027509433431493No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAATAGATCGGAA245260.3920100657367283No Hit
CTAGACTGAAGCTCCTTGAGGAGATCGGAAGAGCACACGT230330.3681467766498435No Hit
TCCTGTACTGAGCTGCCCCTAGATCGGAAGAGCACACGTC223780.3576776176733469No Hit
AAGGGCTTCCTCTCTGCAGGAAGATCGGAAGAGCACACGT196730.3144423886177386No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATTAGATCGGA191760.3064986145546564No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTATTAGAT188910.3019433316412189No Hit
TCCTGTACTGAGCTGCCCCGTAGATCGGAAGAGCACACGT180820.2890127215465841No Hit
TATTGCACTTGTCCCGGCCTGTTAGATCGGAAGAGCACAC168900.26996045055424206No Hit
TCCTGTACTGAGCTGCCCCCAGATCGGAAGAGCACACGTC165850.265085498664423No Hit
TGACATGGGACTGCCTAAGCTAAGATCGGAAGAGCACACG160660.25679008872732106No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATAGATCGGAA158660.2535933989635053No Hit
GATGGATATGTCTTCAAGGATAGATCGGAAGAGCACACGT157310.2514356333729296No Hit
CCCCCCACTGCTAAATTTGACTGGCAGATCGGAAGAGCAC156960.2508762126642619No Hit
TGAGGGGCAGAGAGCGAGACTTTAGATCGGAAGAGCACAC156210.249677454002831No Hit
CGCGACCTCAGATCAGACGTAGATCGGAAGAGCACACGTC146100.23351818724674225No Hit
AGGGCTGGGTCGGTCGGGCTGGGGCGAGATCGGAAGAGCA142620.22795594705770278No Hit
TATTGCACTTGTCCCGGCCTGAAGATCGGAAGAGCACACG133120.2127716706795779No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACGAGATCGGA131110.20955899746694304No Hit
AGCTCGGTCTGAGGCCCCTCAAGATCGGAAGAGCACACGT130640.20880777537244632No Hit
TATTGCACTTGTCCCGGCCTGCAGATCGGAAGAGCACACG130450.2085040898448838No Hit
TGATCTCTTCGTACTCTTCTTGAGATCGGAAGAGCACACG129930.2076729505062917No Hit
CCCCCCACTGCTAAATTTGACTGGTAGATCGGAAGAGCAC123510.1974115763644431No Hit
AAACCGTTACCATTACTGAGATCGGAAGAGCACACGTCTG119590.19114606442736418Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAAAGCTGGGTTGAGAGGGCGAAGATCGGAAGAGCACACG119120.19039484233286746No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAAGATCGGAAG117550.18788544086827208No Hit
TCTCTCGGCTCCTCGCGGCTCAAGATCGGAAGAGCACACG115390.18443301592335104No Hit
TCCTGTACTGAGCTGCCCCGATAGATCGGAAGAGCACACG113730.18177976341938396No Hit
CATTGCACTTGTCTCGGTCTGAGATCGGAAGAGCACACGT110120.1760097383956965No Hit
TCGCTCTGATACCAAATTGATAAGATCGGAAGAGCACACG108700.1737400886633873No Hit
TGAGGGGCAGAGAGCGAGACCAGATCGGAAGAGCACACGT105200.1681458815767097No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTATTAAGA97550.15591854323011434No Hit
TGGAGAGAAAGGCAGTTCCTGAAGATCGGAAGAGCACACG97160.1552951887261703No Hit
TGACTGTGCTGAGTCTGTTCAATCCAACCCTGAGCAGATC92910.14850222297806176No Hit
TGAGGTAGTAGGTTGTGTGGTTAGATCGGAAGAGCACACG88580.1415813896394006No Hit
TGAGGGGCAGAGAGCGAGACAAGATCGGAAGAGCACACGT87730.14022279648977892No Hit
AAACCGTTACCATTACTGAGGAGATCGGAAGAGCACACGT82310.13155976722983817No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAAAGATCGGAAG80170.12813930918255528No Hit
CTTCTCACTACTGCACTTGACTAGTAGATCGGAAGAGCAC79810.12756390502506842No Hit
TCTAGCAGCTGTTGAGCAGGTAGATCGGAAGAGCACACGT79180.12655694774946646No Hit
TGTTTGTTGTACTCGGTCTAGTAGATCGGAAGAGCACACG78810.12596556014316057No Hit
AAGCTGCCAGTTGAAGAACTAGATCGGAAGAGCACACGTC78510.1254860566785882No Hit
TGACATGGGACTGCCTAAGCTAGATCGGAAGAGCACACGT71850.11484107976508166No Hit
AGCTCGGTCTGAGGCCCCTCAGATCGGAAGAGCACACGTC70440.11258741348159154No Hit
TAAGATCCGGACTACAACAAAGAGATCGGAAGAGCACACG70020.11191610863119023No Hit
CTAGACTGAAGCTCCTTGAGAAGATCGGAAGAGCACACGT69860.11166037345008498No Hit
TCCTGTACTGAGCTGCCCCGAGGAGATCGGAAGAGCACAC69760.11150053896189419No Hit
TTTGGCAATGGTAGAACTCACACTAGATCGGAAGAGCACA67170.10736082571775275No Hit
GGCTGGTCCGATGGTAGTAGATCGGAAGAGCACACGTCTG65760.10510715943426263Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGCGACCTCAGATCAGACGTGGCAGATCGGAAGAGCACAC65650.10493134149725275No Hit
TGAGGTAGTAGGTTGTGTGGTAGATCGGAAGAGCACACGT64930.1037805331822791No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)