FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005488296

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005488296
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6547935
Sequences flagged as poor quality0
Sequence length40
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCGTTACCATTACTGAGTAGATCGGAAGAGCACACGT81145712.39256345702882No Hit
TCCTGTACTGAGCTGCCCCGAGAGATCGGAAGAGCACACG5139847.849558677659445No Hit
TCCTGTACTGAGCTGCCCCGAAGATCGGAAGAGCACACGT3997356.104749054472899No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGA3937596.01348364026216No Hit
TATTGCACTTGTCCCGGCCTGTAAGATCGGAAGAGCACAC2222613.394367842686282No Hit
TCCTGTACTGAGCTGCCCCGAGAAGATCGGAAGAGCACAC2081773.1792771308817205No Hit
AAACCGTTACCATTACTGAGCAGATCGGAAGAGCACACGT1568912.396037834828843No Hit
TCCTGTACTGAGCTGCCCCGAGATCGGAAGAGCACACGTC1409292.152266325184963No Hit
TCCTGTACTGAGCTGCCCCGAGTAGATCGGAAGAGCACAC1289921.9699645766184302No Hit
TCCTGTACTGAGCTGCCCCGAAAGATCGGAAGAGCACACG1131021.7272926502782937No Hit
TATTGCACTTGTCCCGGCCTGTAGATCGGAAGAGCACACG1049871.6033604487521638No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACCAGATCGGA761281.1626260798251662No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAAGATCGGA619940.9467717685041162No Hit
CATTGCACTTGTCTCGGTCTGAAGATCGGAAGAGCACACG599530.915601636241044No Hit
TGAGGTAGTAGTTTGTGCTGTTAGATCGGAAGAGCACACG569510.8697551212710573No Hit
TCCTGTACTGAGCTGCCCCAAGATCGGAAGAGCACACGTC511060.7804903377935181No Hit
AAACCGTTACCATTACTGAGAAGATCGGAAGAGCACACGT455770.6960515032601882No Hit
TGAGGGGCAGAGAGCGAGACTAGATCGGAAGAGCACACGT409880.6259683396368473No Hit
TCGCTCTGATACCAAATTGATGAGATCGGAAGAGCACACG398730.6089400704191474No Hit
GATGGATATGTCTTCAAGGACAGATCGGAAGAGCACACGT316540.48341958189872075No Hit
TCTCTCGGCTCCTCGCGGCTCGAGATCGGAAGAGCACACG303540.4635659944700123No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAG289530.44216993601799653TruSeq Adapter, Index 9 (100% over 39bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTAGATCGG273900.41829981513255704No Hit
TTGAATTGAAGTGCTTGAATTAGATCGGAAGAGCACACGT270090.4124811868169125No Hit
TCCTGTACTGAGCTGCCCCGAGCAGATCGGAAGAGCACAC261040.3986600355684655No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAGATCGGAA255830.39070332860665236No Hit
AAACCGTTACCATTACTGAGATCGGAAGAGCACACGTCTG243920.3725143881238895Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTAGACTGAAGCTCCTTGAGGAGATCGGAAGAGCACACGT242140.36979597384518936No Hit
CAACGGAATCCCAAAAGCAGCTAGATCGGAAGAGCACACG234240.3577311014846665No Hit
TATTGCACTTGTCCCGGCCTGTTAGATCGGAAGAGCACAC222080.3391603612436593No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTATTAGAT218850.3342275083671417No Hit
TGACATGGGACTGCCTAAGCTAAGATCGGAAGAGCACACG210280.3211394126545239No Hit
AGCTCGGTCTGAGGCCCCTCAAGATCGGAAGAGCACACGT208740.31878752614373845No Hit
TGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGA198870.3037140716882498No Hit
TCCTGTACTGAGCTGCCCCTAGATCGGAAGAGCACACGTC198020.30241595251021886No Hit
TATTGCACTTGTCCCGGCCTGCAGATCGGAAGAGCACACG197020.3008887534772413No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATTAGATCGGA169540.2589213240510176No Hit
TATTGCACTTGTCCCGGCCTGAAGATCGGAAGAGCACACG166050.2535913994259259No Hit
TCAGTGCACTACAGAACTTTGTAGATCGGAAGAGCACACG164550.25130060087645956No Hit
TCCTGTACTGAGCTGCCCCGTAGATCGGAAGAGCACACGT164030.2505064573793112No Hit
CATTGCACTTGTCTCGGTCTGAGATCGGAAGAGCACACGT156420.23888447273835187No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATAGATCGGAA156310.23871648084472433No Hit
AAGGGCTTCCTCTCTGCAGGAAGATCGGAAGAGCACACGT151930.23202734908028258No Hit
AAACCGTTACCATTACTGAGGAGATCGGAAGAGCACACGT147840.22578110503540427No Hit
TCCTGTACTGAGCTGCCCCGATAGATCGGAAGAGCACACG141310.21580849535006072No Hit
TGAGGGGCAGAGAGCGAGACTTTAGATCGGAAGAGCACAC135750.20731726872670544No Hit
GATGGATATGTCTTCAAGGATAGATCGGAAGAGCACACGT134140.20485847828361153No Hit
AGCTCGGTCTGAGGCCCCTCAGATCGGAAGAGCACACGTC122960.18778439309492231No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTATTAAGA118910.1815992370113631No Hit
TCTCTCGGCTCCTCGCGGCTCAAGATCGGAAGAGCACACG117560.17953751831684342No Hit
TGATCTCTTCGTACTCTTCTTGAGATCGGAAGAGCACACG114180.17437558558537922No Hit
TGAGGTAGTAGTTTGTGCTGTCAGATCGGAAGAGCACACG106560.1627383289540901No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACGAGATCGGA106470.1626008810411221No Hit
TGTAAACATCCCCGACTGGAAGCTAGATCGGAAGAGCACA106150.1621121773505693No Hit
CCCCCCACTGCTAAATTTGACTGGCAGATCGGAAGAGCAC105110.16052389035627262No Hit
TTTGGCAATGGTAGAACTCACACTAGATCGGAAGAGCACA103960.15876761146834842No Hit
CGCGACCTCAGATCAGACGTAGATCGGAAGAGCACACGTC103020.1573320443773495No Hit
AGCTCGGTCTGAGGCCCCTCAGTAGATCGGAAGAGCACAC101480.15498015786656402No Hit
TGTTTGTTGTACTCGGTCTAGTAGATCGGAAGAGCACACG97430.14879500178300487No Hit
TGGAGAGAAAGGCAGTTCCTGAAGATCGGAAGAGCACACG96490.14735943469200594No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAAAGATCGGAAG95980.1465805631851874No Hit
TCGCTCTGATACCAAATTGATAAGATCGGAAGAGCACACG92920.14190733414427603No Hit
TATTGCACTTGTCCCGGCCTGAGATCGGAAGAGCACACGT84100.1284374386734138No Hit
CTTCTCACTACTGCACTTGACTAGTAGATCGGAAGAGCAC83200.127062959543734No Hit
TGAGGGGCAGAGAGCGAGACCAGATCGGAAGAGCACACGT80200.1224813624448013No Hit
TCTAGCAGCTGTTGAGCAGGTAGATCGGAAGAGCACACGT79070.12075562753753664No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAATAGATCGGAA77840.11887717272697423No Hit
AAACCGTTACCATTACTGAGAGATCGGAAGAGCACACGTC77530.11840374102675118No Hit
TAAGATCCGGACTACAACAAAGAGATCGGAAGAGCACACG75800.11576168669969998No Hit
AAGCTGCCAGTTGAAGAACTAGATCGGAAGAGCACACGTC75260.11493699922189209No Hit
TCCTGTACTGAGCTGCCCCGAGGAGATCGGAAGAGCACAC75000.11453992747331793No Hit
TGACATGGGACTGCCTAAGCTAGATCGGAAGAGCACACGT72650.11095100974582062No Hit
CTAGACTGAAGCTCCTTGAGAAGATCGGAAGAGCACACGT70760.10806460357349303No Hit
TCCTGTACTGAGCTGCCCCGGAGATCGGAAGAGCACACGT69780.106567948521175No Hit
TGAGGGGCAGAGAGCGAGACAAGATCGGAAGAGCACACGT68720.10494911754621877No Hit
TGAGGGGCAGAGCGCGAGACTAGATCGGAAGAGCACACGT68360.10439932589434685No Hit
AAACCGTTACCATTACTAAGATCGGAAGAGCACACGTCTG67750.10346773448423052Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCTGTACTGAGCTGCCCCCAGATCGGAAGAGCACACGTC67470.1030401187549968No Hit
TCGCTCTGATACCCAATTGATGAGATCGGAAGAGCACACG67120.10250559909345466No Hit
CTTCTCACTACTGCACTTGACTAGTCAGATCGGAAGAGCA66290.10123802389608327No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)