FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005488297

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005488297
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12467216
Sequences flagged as poor quality0
Sequence length40
%GC56

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTGTACTGAGCTGCCCCGAGAGATCGGAAGAGCACACG247282919.834652740435395No Hit
TCCTGTACTGAGCTGCCCCGAAGATCGGAAGAGCACACGT129213410.364254537661015No Hit
TCCTGTACTGAGCTGCCCCGAGATCGGAAGAGCACACGTC8813287.069164438957342No Hit
TCCTGTACTGAGCTGCCCCGAGAAGATCGGAAGAGCACAC7944256.372112266283025No Hit
TCCTGTACTGAGCTGCCCCGAAAGATCGGAAGAGCACACG5640254.524065356692304No Hit
TCCTGTACTGAGCTGCCCCGAGTAGATCGGAAGAGCACAC4562943.659951026756896No Hit
AAACCGTTACCATTACTGAGTAGATCGGAAGAGCACACGT3794493.043574443564626No Hit
TATTGCACTTGTCCCGGCCTGTAAGATCGGAAGAGCACAC3108322.493194952265205No Hit
TCCTGTACTGAGCTGCCCCAAGATCGGAAGAGCACACGTC2940932.358930815027188No Hit
TATTGCACTTGTCCCGGCCTGTAGATCGGAAGAGCACACG2314591.8565411877038145No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGA1289841.0345854278934445No Hit
TCCTGTACTGAGCTGCCCCTAGATCGGAAGAGCACACGTC1218330.9772269927785No Hit
TCCTGTACTGAGCTGCCCCGAGCAGATCGGAAGAGCACAC931110.7468467699605108No Hit
CATTGCACTTGTCTCGGTCTGAAGATCGGAAGAGCACACG849500.6813870875422388No Hit
CTAGACTGAAGCTCCTTGAGGAGATCGGAAGAGCACACGT763900.6127270113873058No Hit
TCCTGTACTGAGCTGCCCCCAGATCGGAAGAGCACACGTC756140.6065026867265314No Hit
AAACCGTTACCATTACTGAGCAGATCGGAAGAGCACACGT739980.5935406910412077No Hit
CAACGGAATCCCAAAAGCAGCTAGATCGGAAGAGCACACG705480.5658681136189507No Hit
TCCTGTACTGAGCTGCCCCGATAGATCGGAAGAGCACACG642350.5152313074546876No Hit
TCCTGTACTGAGCTGCCCCGTAGATCGGAAGAGCACACGT543620.43603960980542883No Hit
TGAGGGGCAGAGAGCGAGACTAGATCGGAAGAGCACACGT537390.43104250379555464No Hit
TATTGCACTTGTCCCGGCCTGCAGATCGGAAGAGCACACG438650.351842785109362No Hit
TCTCTCGGCTCCTCGCGGCTCGAGATCGGAAGAGCACACG436250.349917736245205No Hit
TATTGCACTTGTCCCGGCCTGAAGATCGGAAGAGCACACG435900.34963699995251546No Hit
TATTGCACTTGTCCCGGCCTGTTAGATCGGAAGAGCACAC400580.32130669750167157No Hit
TCGCTCTGATACCAAATTGATGAGATCGGAAGAGCACACG372730.2989681096405164No Hit
CTTCTCACTACTGCACTTGACTAGTAGATCGGAAGAGCAC352690.2828939516248054No Hit
CCCCCCACTGCTAAATTTGACTGGCAGATCGGAAGAGCAC333220.2672769927143317No Hit
AGCTCGGTCTGAGGCCCCTCAAGATCGGAAGAGCACACGT315950.2534246619293353No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAA315310.2529113155655601TruSeq Adapter, Index 13 (100% over 39bp)
AGCTCGGTCTGAGGCCCCTCAGTAGATCGGAAGAGCACAC292250.2344148043957849No Hit
TCCTGTACTGAGCTGCCCCGAGGAGATCGGAAGAGCACAC289280.23203255642639062No Hit
TATTGCACTTGTCCCGGCCTGAGATCGGAAGAGCACACGT270350.21684873351035228No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACCAGATCGGA244860.1964031103656181No Hit
TGAGGGGCAGAGAGCGAGACTTTAGATCGGAAGAGCACAC235200.1886547886873862No Hit
AAACCGTTACCATTACTGAGAAGATCGGAAGAGCACACGT233170.18702651818978672No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAAGATCGGA220620.17696011683763238No Hit
TCCTGTACTGAGCTGCCCCGGAGATCGGAAGAGCACACGT219240.17585321374074211No Hit
CCCCCCACTGCTAAATTTGACTGGTAGATCGGAAGAGCAC218290.17509121523201332No Hit
TCCTGTACTGAGCTGCCCCGACAGATCGGAAGAGCACACG211780.16986952018798746No Hit
CTAGACTGAAGCTCCTTGAGAAGATCGGAAGAGCACACGT211390.1695566997475619No Hit
CATTGCACTTGTCTCGGTCTGAGATCGGAAGAGCACACGT193360.15509477015558246No Hit
CTAGACTGAAGCTCCTTGAGGAAGATCGGAAGAGCACACG185220.14856564609131662No Hit
CTTCTCACTACTGCACTTGACTAGTCAGATCGGAAGAGCA177850.1426541418709678No Hit
AGCTCGGTCTGAGGCCCCTCAGATCGGAAGAGCACACGTC177370.14226913209813644No Hit
TCTCTCGGCTCCTCGCGGCTCAAGATCGGAAGAGCACACG171200.13732015230986613No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATTAGATCGGA164230.13172948956687686No Hit
GATGGATATGTCTTCAAGGACAGATCGGAAGAGCACACGT157290.1261628899346895No Hit
TGACTGTGCTGAGTCTGTTCAATCCAACCCTGAGCAGATC155420.12466295602803383No Hit
CAACGGAATCCCAAAAGCAGCCAGATCGGAAGAGCACACG144200.11566335258809987No Hit
TCCCGTACTGAGCTGCCCCGAGAGATCGGAAGAGCACACG143290.114933438227107No Hit
CAACGGAATCCCAAAAGCAGCAAGATCGGAAGAGCACACG139890.11220628566955125No Hit
TGAGGTAGTAGTTTGTGCTGTTAGATCGGAAGAGCACACG135050.1083241037935013No Hit
TGATCTCTTCGTACTCTTCTTGAGATCGGAAGAGCACACG134400.1078027363927921No Hit
CAACGGAATCCCAAAAGCAGCTGAGATCGGAAGAGCACAC128750.10327085052508916No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)