FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005488299

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005488299
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7506913
Sequences flagged as poor quality0
Sequence length40
%GC56

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTGTACTGAGCTGCCCCGAGAGATCGGAAGAGCACACG143826119.15915370272707No Hit
TCCTGTACTGAGCTGCCCCGAAGATCGGAAGAGCACACGT6729578.964497124184069No Hit
TCCTGTACTGAGCTGCCCCGAGAAGATCGGAAGAGCACAC6246778.321356594914581No Hit
TCCTGTACTGAGCTGCCCCGAGTAGATCGGAAGAGCACAC5361137.141590691140285No Hit
TCCTGTACTGAGCTGCCCCGAGATCGGAAGAGCACACGTC2996023.99101468206705No Hit
TATTGCACTTGTCCCGGCCTGTAGATCGGAAGAGCACACG2314663.0833712872388426No Hit
AAACCGTTACCATTACTGAGTAGATCGGAAGAGCACACGT1759792.344225915499487No Hit
CATTGCACTTGTCTCGGTCTGAAGATCGGAAGAGCACACG1597682.128278294952932No Hit
TATTGCACTTGTCCCGGCCTGTAAGATCGGAAGAGCACAC1587782.1150904506286405No Hit
TCCTGTACTGAGCTGCCCCGAAAGATCGGAAGAGCACACG1418241.8892452863114306No Hit
TCCTGTACTGAGCTGCCCCGAGCAGATCGGAAGAGCACAC733600.9772325854848725No Hit
TCCTGTACTGAGCTGCCCCAAGATCGGAAGAGCACACGTC650780.8669076090265065No Hit
TGTAAACATCCCCGACTGGAAGCTAGATCGGAAGAGCACA491880.6552360470941918No Hit
CATTGCACTTGTCTCGGTCTGAGATCGGAAGAGCACACGT396890.5286993468553585No Hit
TGAGGGGCAGAGAGCGAGACTAGATCGGAAGAGCACACGT387970.5168169659086232No Hit
TATTGCACTTGTCCCGGCCTGCAGATCGGAAGAGCACACG367270.4892423823214682No Hit
AGCTCGGTCTGAGGCCCCTCAGTAGATCGGAAGAGCACAC364520.48557909223138723No Hit
TCTCTCGGCTCCTCGCGGCTCGAGATCGGAAGAGCACACG342330.45601967146815214No Hit
CTAGACTGAAGCTCCTTGAGGAGATCGGAAGAGCACACGT300030.3996716093552703No Hit
CGCGACCTCAGATCGGAAGAGCACACGTCTGAACTCCAGT295750.39397019786961696Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TATTGCACTTGTCCCGGCCTGTTAGATCGGAAGAGCACAC288330.38408597515383486No Hit
CAACGGAATCCCAAAAGCAGCTAGATCGGAAGAGCACACG279310.3720703836583693No Hit
TATTGCACTTGTCCCGGCCTGAAGATCGGAAGAGCACACG256130.34119217846270494No Hit
TTGAATTGAAGTGCTTGAATTAGATCGGAAGAGCACACGT246350.32816418679688975No Hit
AAACCGTTACCATTACTGAGCAGATCGGAAGAGCACACGT225660.30060292426460783No Hit
TCCTGTACTGAGCTGCCCCTAGATCGGAAGAGCACACGTC212800.2834720476979019No Hit
TATTGCACTTGTCCCGGCCTGAGATCGGAAGAGCACACGT211100.28120746836948823No Hit
TGACATGGGACTGCCTAAGCTAAGATCGGAAGAGCACACG206730.27538616738997773No Hit
ATTGCACTTGTCCCGGCCTGTAGATCGGAAGAGCACACGT198600.26455614977821107No Hit
TCCTGTACTGAGCTGCCCCGATAGATCGGAAGAGCACACG196800.26215835990106723No Hit
AGCTCGGTCTGAGGCCCCTCAGATCGGAAGAGCACACGTC178300.23751440838597704No Hit
TCCTGTACTGAGCTGCCCCGTAGATCGGAAGAGCACACGT167990.22378040081189166No Hit
TCCTGTACTGAGCTGCCCCGAGGAGATCGGAAGAGCACAC167000.2224616163794625No Hit
AGCTCGGTCTGAGGCCCCTCAAGATCGGAAGAGCACACGT142890.1903445530806072No Hit
CAACGGAATCCCAAAAGCAGCTGAGATCGGAAGAGCACAC121850.1623170536277695No Hit
CAAAGAATTCTCCTTTTGGGCTAGATCGGAAGAGCACACG117830.15696198956881477No Hit
TCCTGTACTGAGCTGCCCCGGGAGATCGGAAGAGCACACG113340.15098083593082803No Hit
TCCTGTACTGAGCTGCCCCCAGATCGGAAGAGCACACGTC110570.147290903731001No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGA110370.1470244826335406No Hit
TGAGGGGCAGAGAGCGAGACTTTAGATCGGAAGAGCACAC107570.1432945872690945No Hit
TGGAGAGAAAGGCAGTTCCTGAAGATCGGAAGAGCACACG105240.14019078148368044No Hit
TCCTGTACTGAGCTGCCCCGGAGATCGGAAGAGCACACGT104140.13872546544764805No Hit
AAACCGTTACCCTTACTGAGTAGATCGGAAGAGCACACGT104000.13853897067942575No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTC97260.12956057969500911TruSeq Adapter, Index 6 (100% over 40bp)
ACCACAGGGTAGAACCACGGAAGATCGGAAGAGCACACGT96050.1279487320553735No Hit
GATGGATATGTCTTCAAGGACAGATCGGAAGAGCACACGT93640.12473835783097527No Hit
TGAGGGGCAGAGAGCGAGACTTAGATCGGAAGAGCACACG93380.12439201040427669No Hit
TCGCTCTGATACCAAATTGATGAGATCGGAAGAGCACACG90830.12099514141165617No Hit
TGTAAACATCCCCGACTGGAAGAGATCGGAAGAGCACACG89680.11946322010125868No Hit
TGAGGGGCAGAGCGCGAGACTAGATCGGAAGAGCACACGT88100.11735849343132124No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAAT85910.11444118241412948TruSeq Adapter, Index 6 (100% over 39bp)
CTAGACTGAAGCTCCTTGAGAGATCGGAAGAGCACACGTC85190.11348206646327193No Hit
TCCTGTACTGAGCGTCGCTAGATCGGAAGAGCACACGTCT84800.11296254532322408Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCTCTCGGCTCCTCGCGGCTCAAGATCGGAAGAGCACACG84750.11289594004885897No Hit
AAGGGCTTCCTCTCTGCAGGAAGATCGGAAGAGCACACGT84720.1128559768842399No Hit
TGTAAACATCCCCGACTGGAAGCCAGATCGGAAGAGCACA82490.10988538164755607No Hit
CATTGCACTTGTCTCGGTCTGTAGATCGGAAGAGCACACG81860.10904615519055569No Hit
TCCCGTACTGAGCTGCCCCGAGAGATCGGAAGAGCACACG81080.10800711291046No Hit
TGAGGTAGTAGTTTGTGCTGTTAGATCGGAAGAGCACACG80020.1065950810939197No Hit
CTAGACTGAAGCTCCTTGAGGTAGATCGGAAGAGCACACG77780.10361116480236283No Hit
TGTAAACATCCCCGACTGGAAGCAGATCGGAAGAGCACAC76960.10251883830277506No Hit
CCCTGTACTGAGCTGCCCCGAGAGATCGGAAGAGCACACG76690.10215916982120347No Hit
TTTGGCAATGGTAGAACTCACACTAGATCGGAAGAGCACA76060.1013199433642031No Hit
AAACCGTTACCATTACTGAGTTAGATCGGAAGAGCACACG75290.10029422213898043No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAAACG200.002787612134.0058534
CACAACG351.0909498E-634.0058534
ACACATG504.456524E-1034.00584834
GACAACA200.002787885334.0051733
CAACGGG455.991751E-934.0021
CGAAACT1100.034.0021
CTATACG3000.034.0021
TATGGCA1100.034.0021
CGTTGCA1050.034.0021
TTCACGT200.002789160234.0021
TAAACTA351.0919157E-634.0021
TTCAAGT10550.034.0021
TCGATTC1150.034.0021
TAACGGA408.0717655E-834.0021
TGTGCAA301.48417785E-534.0021
TATGACA200.002789160234.0021
AAGCCGT950.034.0021
TATTACA750.034.0021
GTCCGGA200.002789160234.00225
TCGAAAC1200.034.0021
Produced by FastQC (version 0.11.5)