FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005488300

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005488300
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6367431
Sequences flagged as poor quality0
Sequence length40
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTGTACTGAGCTGCCCCGAGAGATCGGAAGAGCACACG89606414.072614214429649No Hit
TCCTGTACTGAGCTGCCCCGAAGATCGGAAGAGCACACGT4872857.652772366123795No Hit
TCCTGTACTGAGCTGCCCCGAGAAGATCGGAAGAGCACAC4181366.566792792886174No Hit
TCCTGTACTGAGCTGCCCCGAGTAGATCGGAAGAGCACAC2631674.133016910587646No Hit
AAACCGTTACCATTACTGAGTAGATCGGAAGAGCACACGT2623244.1197776622942595No Hit
TATTGCACTTGTCCCGGCCTGTAGATCGGAAGAGCACACG2465773.8724722733548274No Hit
TCCTGTACTGAGCTGCCCCGAGATCGGAAGAGCACACGTC1975423.1023814784958015No Hit
TCCTGTACTGAGCTGCCCCGAAAGATCGGAAGAGCACACG1266301.9887141297644215No Hit
TATTGCACTTGTCCCGGCCTGTAAGATCGGAAGAGCACAC1250411.9637590105020377No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGA946821.4869733178105895No Hit
CAACGGAATCCCAAAAGCAGCTAGATCGGAAGAGCACACG677181.0635058314726928No Hit
CATTGCACTTGTCTCGGTCTGAAGATCGGAAGAGCACACG582840.9153456079853869No Hit
TGAGGGGCAGAGAGCGAGACTAGATCGGAAGAGCACACGT517720.8130751632801361No Hit
TCCTGTACTGAGCTGCCCCAAGATCGGAAGAGCACACGTC472720.7424030193652668No Hit
TATTGCACTTGTCCCGGCCTGCAGATCGGAAGAGCACACG400540.6290449005258164No Hit
TATTGCACTTGTCCCGGCCTGTTAGATCGGAAGAGCACAC371170.5829195479307118No Hit
TCCTGTACTGAGCTGCCCCGAGCAGATCGGAAGAGCACAC361910.5683767912051186No Hit
TGTAAACATCCCCGACTGGAAGCTAGATCGGAAGAGCACA339910.5338259652911825No Hit
AAACCGTTACCATTACTGAGCAGATCGGAAGAGCACACGT337890.5306535712754484No Hit
TCTCTCGGCTCCTCGCGGCTCGAGATCGGAAGAGCACACG331710.5209479301778064No Hit
TTGAATTGAAGTGCTTGAATTAGATCGGAAGAGCACACGT281930.44276883408709106No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAGATCGGAA271610.426561355749281No Hit
TATTGCACTTGTCCCGGCCTGAAGATCGGAAGAGCACACG238630.3747665267201168No Hit
TGACATGGGACTGCCTAAGCTAAGATCGGAAGAGCACACG213650.33553563438692935No Hit
ATTGCACTTGTCCCGGCCTGTAGATCGGAAGAGCACACGT209340.3287668134919719No Hit
TGAGGTAGTAGTTTGTGCTGTTAGATCGGAAGAGCACACG203070.3189198281065001No Hit
TATTGCACTTGTCCCGGCCTGAGATCGGAAGAGCACACGT197510.31018789210279624No Hit
AAACCGTTACCATTACTGAGTTAGATCGGAAGAGCACACG164660.25859722704494165No Hit
TCCTGTACTGAGCTGCCCCGTAGATCGGAAGAGCACACGT161450.253555947445681No Hit
TGGAGAGAAAGGCAGTTCCTGAAGATCGGAAGAGCACACG159500.25049348787603665No Hit
TCCTGTACTGAGCTGCCCCGATAGATCGGAAGAGCACACG150170.23584079670435376No Hit
TCCTGTACTGAGCTGCCCCTAGATCGGAAGAGCACACGTC144800.22740725419717936No Hit
TCAGGCTCAGTCCCCTCCCGATAGATCGGAAGAGCACACG143680.22564830305974262No Hit
TGAGGGGCAGAGAGCGAGACTTTAGATCGGAAGAGCACAC142940.22448613891536476No Hit
CAACGGAATCCCACAAGCAGCTAGATCGGAAGAGCACACG142450.2237165977927362No Hit
GGCTGGTCCGATGGTAGTGGGTTATCCGAACTAGATCGGA135510.21281738270897635No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACCAGATCGGA133010.2088911524914836No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACGTAGAGATCTC128980.20256206938088533TruSeq Adapter, Index 3 (96% over 33bp)
CATTGCACTTGTCTCGGTCTGAGATCGGAAGAGCACACGT127940.20092875761040835No Hit
TCCTGTACTGAGCTGCCCCGAGGAGATCGGAAGAGCACAC127110.19962524917820074No Hit
AGCTCGGTCTGAGGCCCCTCAGTAGATCGGAAGAGCACAC123620.19414423179458087No Hit
CGCGACCTCAGATCGGAAGAGCACACGTCTGAACTCCAGT118570.18621324675524556Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GATGGATATGTCTTCAAGGACAGATCGGAAGAGCACACGT118010.1853337711865272No Hit
TCCTGTACTGAGCGTCGCTAGATCGGAAGAGCACACGTCT117470.18448570545954876Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CAACGGAATCCCAAAAGCAGCCAGATCGGAAGAGCACACG113210.17779540916894113No Hit
CCCCCCACTGCTAAATTTGACTGGCTTAGATCGGAAGAGC112260.1763034416862939No Hit
TGTAAACATCCCCGACTGGAAAGATCGGAAGAGCACACGT112060.17598934326889448No Hit
TCGCTCTGATACCAAATTGATGAGATCGGAAGAGCACACG108280.17005288318004544No Hit
ACCACAGGGTAGAACCACGGAAGATCGGAAGAGCACACGT103900.16317412783899818No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAGAG102570.16108537336329204TruSeq Adapter, Index 3 (97% over 37bp)
TGAGGGGCAGAGAGCGAGACTTAGATCGGAAGAGCACACG99880.15686074964926985No Hit
AAAAGCTGGGTTGAGAGGGCGAAGATCGGAAGAGCACACG98680.15497615914487334No Hit
TCTCTCGGCTCCTCGCGGCTCAAGATCGGAAGAGCACACG97500.15312297848221676No Hit
TCCTGTACTGAGCTGCCCCCAGATCGGAAGAGCACACGTC96220.1511127486108605No Hit
AGCTCGGTCTGAGGCCCCTCAAGATCGGAAGAGCACACGT94070.14773619062381674No Hit
CAAAGAATTCTCCTTTTGGGCTAGATCGGAAGAGCACACG92910.1459144198029001No Hit
CAACGGAATCCCAAAAGCAGCTGAGATCGGAAGAGCACAC89430.1404491073401502No Hit
AAACCGTTACCATTACTGAGAGATCGGAAGAGCACACGTC88970.13972668098013155No Hit
TGAGGTAGTAGGTTGTGTGGTTAGATCGGAAGAGCACACG88100.13836035286444406No Hit
TGTAAACATCCCCGACTGGAAGATCGGAAGAGCACACGTC84910.13335048310692335No Hit
TGATTGGAAATTTCGTTGACTAGATCGGAAGAGCACACGT83760.13154441720687668No Hit
TGTAAACATCCCCGACTGGAAGAGATCGGAAGAGCACACG82420.12943995781030057No Hit
TCCTGTACTGAGCTGCCCCGGAGATCGGAAGAGCACACGT82330.12929861352247082No Hit
AAGCTGCCAGTTGAAGAACTGTAGATCGGAAGAGCACACG80630.12662877697457575No Hit
AAGCTGCCAGTTGAAGAACTAGATCGGAAGAGCACACGTC80440.12633038347804632No Hit
TCAGGCTCAGTCCCCTCCCGAAGATCGGAAGAGCACACGT80210.125969170298037No Hit
TGAGGGGCAGAGAGCGAGACCAGATCGGAAGAGCACACGT77670.12198012039706438No Hit
CGCGACCTCAGATCAGACGTAGATCGGAAGAGCACACGTC75390.118399398438711No Hit
TGAGGGGCAGAGCGCGAGACTAGATCGGAAGAGCACACGT72890.11447316822121825No Hit
AAACCGTTACCATTACTGAGATCGGAAGAGCACACGTCTG71160.11175621691071329Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTAGACTGAAGCTCCTTGAGGAGATCGGAAGAGCACACGT70440.11062546260807538No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTAGATCGG69610.10932195417586779No Hit
AGCTCGGTCTGAGGCCCCTCAGATCGGAAGAGCACACGTC69580.10927483941325787No Hit
AAGGGCTTCCTCTCTGCAGGAAGATCGGAAGAGCACACGT69120.1085524130532392No Hit
CCCCCCACTGCTAAATTTGACTGGCAGATCGGAAGAGCAC68940.10826972447757974No Hit
TACCACAGGGTAGAACCACGGAAGATCGGAAGAGCACACG65360.10264736280613013No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)