FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005488301

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005488301
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6251603
Sequences flagged as poor quality0
Sequence length40
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCGTTACCATTACTGAGTAGATCGGAAGAGCACACGT72735411.634679937289684No Hit
TCCTGTACTGAGCTGCCCCGAGAGATCGGAAGAGCACACG5002748.002331562000977No Hit
TCCTGTACTGAGCTGCCCCGAAGATCGGAAGAGCACACGT3077054.922017600925715No Hit
TATTGCACTTGTCCCGGCCTGTAGATCGGAAGAGCACACG2461243.936974244845682No Hit
TCCTGTACTGAGCTGCCCCGAGAAGATCGGAAGAGCACAC2145863.432495633519915No Hit
TCCTGTACTGAGCTGCCCCGAGTAGATCGGAAGAGCACAC1995523.1920133124256287No Hit
TATTGCACTTGTCCCGGCCTGTAAGATCGGAAGAGCACAC1142751.8279311722129508No Hit
TCCTGTACTGAGCTGCCCCGAGATCGGAAGAGCACACGTC1114111.7821189221388498No Hit
AAACCGTTACCATTACTGAGCAGATCGGAAGAGCACACGT1026331.6417069350053097No Hit
CAACGGAATCCCAAAAGCAGCTAGATCGGAAGAGCACACG904741.4472128188562199No Hit
CATTGCACTTGTCTCGGTCTGAAGATCGGAAGAGCACACG835371.3362492787849771No Hit
TCCTGTACTGAGCTGCCCCGAAAGATCGGAAGAGCACACG716301.1457861287736921No Hit
AAACCGTTACCATTACTGAGTTAGATCGGAAGAGCACACG492780.7882458307093396No Hit
TATTGCACTTGTCCCGGCCTGCAGATCGGAAGAGCACACG432800.6923024382706323No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGA425220.680177548062473No Hit
TATTGCACTTGTCCCGGCCTGTTAGATCGGAAGAGCACAC412020.6590629635311135No Hit
TGAGGGGCAGAGAGCGAGACTAGATCGGAAGAGCACACGT401080.6415634518058808No Hit
TGTAAACATCCCCGACTGGAAGCTAGATCGGAAGAGCACA368130.5888569699643436No Hit
TTGAATTGAAGTGCTTGAATTAGATCGGAAGAGCACACGT357150.5712934746496219No Hit
TGACATGGGACTGCCTAAGCTAAGATCGGAAGAGCACACG330090.528008576360335No Hit
TCCTGTACTGAGCTGCCCCGAGCAGATCGGAAGAGCACAC305200.48819478780082487No Hit
TCCTGTACTGAGCTGCCCCAAGATCGGAAGAGCACACGTC287800.4603619263731238No Hit
AAACCGTTACCATTACTGAGAGATCGGAAGAGCACACGTC261420.41816474910514945No Hit
CGCGACCTCAGATCGGAAGAGCACACGTCTGAACTCCAGT257970.41264616451172603Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TATTGCACTTGTCCCGGCCTGAAGATCGGAAGAGCACACG249270.3987297337978755No Hit
TGGAGAGAAAGGCAGTTCCTGAAGATCGGAAGAGCACACG219280.35075803757852186No Hit
AAACCGTTACCATTACTGAGATCGGAAGAGCACACGTCTG217640.34813471040947414Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CATTGCACTTGTCTCGGTCTGAGATCGGAAGAGCACACGT209110.33449020995095174No Hit
ATTGCACTTGTCCCGGCCTGTAGATCGGAAGAGCACACGT202690.32422084383797245No Hit
TATTGCACTTGTCCCGGCCTGAGATCGGAAGAGCACACGT202230.3234850325588493No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACGTGAAAATCTC195880.3133276377274756TruSeq Adapter, Index 19 (97% over 40bp)
CAACGGAATCCCACAAGCAGCTAGATCGGAAGAGCACACG191770.3067533239074842No Hit
ACCACAGGGTAGAACCACGGAAGATCGGAAGAGCACACGT183490.29350872088326785No Hit
TCTCTCGGCTCCTCGCGGCTCGAGATCGGAAGAGCACACG178200.28504689117335186No Hit
CAACGGAATCCCAAAAGCAGCCAGATCGGAAGAGCACACG164280.262780602031191No Hit
GATGGATATGTCTTCAAGGACAGATCGGAAGAGCACACGT163830.26206078664944016No Hit
TGTAAACATCCCCGACTGGAAAGATCGGAAGAGCACACGT163180.26102105332024444No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAGATCGGAA155780.24918408926478536No Hit
AAACCGTTACCATTACTGAGTTTAGATCGGAAGAGCACAC155610.24891215900945726No Hit
TCAGGCTCAGTCCCCTCCCGATAGATCGGAAGAGCACACG150060.24003443596786297No Hit
TGAGGTAGTAGTTTGTGCTGTTAGATCGGAAGAGCACACG149150.23857880930698894No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAA148150.23697921956976475TruSeq Adapter, Index 19 (100% over 39bp)
AAACCGTTACCATTACTGAGAAGATCGGAAGAGCACACGT143080.22886929960203806No Hit
TCGCTCTGATACCAAATTGATGAGATCGGAAGAGCACACG140660.22499829243795552No Hit
AAGCTGCCAGTTGAAGAACTGTAGATCGGAAGAGCACACG138550.22162315809241248No Hit
TACCACAGGGTAGAACCACGGAAGATCGGAAGAGCACACG131680.21063397659768224No Hit
TGAGGGGCAGAGAGCGAGACTTTAGATCGGAAGAGCACAC129380.20695492020206657No Hit
CAACGGAATCCCAAAAGCAGCTGAGATCGGAAGAGCACAC125810.2012443848401762No Hit
TGTAAACATCCCCGACTGGAAGATCGGAAGAGCACACGTC125370.20054056535579756No Hit
TGTAAACATCCCCGACTGGAAGAGATCGGAAGAGCACACG116100.18571236849172923No Hit
CAAAGAATTCTCCTTTTGGGCTAGATCGGAAGAGCACACG111260.17797035416356413No Hit
AAGCTGCCAGTTGAAGAACTAGATCGGAAGAGCACACGTC110710.17709057980809081No Hit
TCCTGTACTGAGCTGCCCCGTAGATCGGAAGAGCACACGT103570.16566950908431005No Hit
TGATTGGAAATTTCGTTGACTAGATCGGAAGAGCACACGT102510.1639739439628524No Hit
AAACCGTTACCATTACTGAGGAGATCGGAAGAGCACACGT99860.15973503115920828No Hit
TCCTGTACTGAGCTGCCCCTAGATCGGAAGAGCACACGTC96980.1551282127160026No Hit
TCCTGTACTGAGCTGCCCCGATAGATCGGAAGAGCACACG96450.15428043015527376No Hit
TCAGGCTCAGTCCCCTCCCGAAGATCGGAAGAGCACACGT96020.15359260656826737No Hit
TGAGGGGCAGAGAGCGAGACTTAGATCGGAAGAGCACACG91620.1465544117244809No Hit
AGCTCGGTCTGAGGCCCCTCAGTAGATCGGAAGAGCACAC91560.14645843634024744No Hit
CGCGACCTCAGATCAGACGTAGATCGGAAGAGCACACGTC88660.14181962610229729No Hit
TTTGGCAATGGTAGAACTCACACTAGATCGGAAGAGCACA88090.1409078599520795No Hit
TCCTGTACTGAGCGTCGCTAGATCGGAAGAGCACACGTCT81290.13003064973895495Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGCGACCTTAGATCGGAAGAGCACACGTCTGAACTCCAGT80060.1280631543621692Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CTAGACTGAAGCTCCTTGAGGAGATCGGAAGAGCACACGT77150.12340834822684678No Hit
CCCCCCACTGCTAAATTTGACTGGCTTAGATCGGAAGAGC74910.11982526721546459No Hit
AAGGGCTTCCTCTCTGCAGGAAGATCGGAAGAGCACACGT73010.1167860467147386No Hit
CAACGGAATCCCAAAAGCAGCAAGATCGGAAGAGCACACG72850.11653011235678273No Hit
AATGACACGATCACTCCCGTAGATCGGAAGAGCACACGTC72840.11651411645941048No Hit
CAACGGAATCCCCAAAGCAGCTAGATCGGAAGAGCACACG72660.11622619030671012No Hit
TCCTGTACTGAGCTGCCCCGAGGAGATCGGAAGAGCACAC72210.11550637492495924No Hit
TTATGTCTTGTTGATCTCAATAGATCGGAAGAGCACACGT72090.11531442415649235No Hit
AAACCGTTACCATTACTGAGTCAGATCGGAAGAGCACACG70420.11264310929532795No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACCAGATCGGA68790.11003577802365248No Hit
AAAAGCTGGGTTGAGAGGGCGAAGATCGGAAGAGCACACG67490.10795631136526103No Hit
AAACCGTTACCCTTACTGAGTAGATCGGAAGAGCACACGT67450.10789232777577208No Hit
TATTGCACTTGTCCCGGCCTGTCAGATCGGAAGAGCACAC67170.1074444426493493No Hit
TGTAAACATCCCCGACTGGAAGCCAGATCGGAAGAGCACA66820.10688458624132083No Hit
CCCCCCACTGCTAAATTTGACTGGCAGATCGGAAGAGCAC66110.10574887752789165No Hit
TCCCCGGCATCTCCACCAAGATCGGAAGAGCACACGTCTG66050.10565290214365819Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGAGGGGCAGAGAGCGAGACCAGATCGGAAGAGCACACGT65890.10539696778570232No Hit
CCCATAAAGTAGAAAGCACTAGATCGGAAGAGCACACGTC64790.10363741907475571No Hit
AGCTCGGTCTGAGGCCCCTCAAGATCGGAAGAGCACACGT63240.10115805498205821No Hit
GGCTGGTCCGATGGTAGTGGGTTATCCGAACTAGATCGGA63060.10087012882935785No Hit
CATTGCACTTGTCTCGGTCTGTAGATCGGAAGAGCACACG62770.10040624780556283No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)