FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005488308

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005488308
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10177190
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCGTTACCATTACTGAGTAGATCGGAAGAGCACACGTCTGAACTCCA368863636.24414990778397Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AAACCGTTACCATTACTGAGCAGATCGGAAGAGCACACGTCTGAACTCCA4907964.82250994626218Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCCTGTACTGAGCTGCCCCGAGAGATCGGAAGAGCACACGTCTGAACTCC2971132.9194011313535464Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCTGTACTGAGCTGCCCCGAAGATCGGAAGAGCACACGTCTGAACTCCA2029791.9944503345225941Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TATTGCACTTGTCCCGGCCTGTAGATCGGAAGAGCACACGTCTGAACTCC1756141.7255647187484953Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCTGTACTGAGCTGCCCCGAGTAGATCGGAAGAGCACACGTCTGAACTC1416321.3916611559772392Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCCTGTACTGAGCTGCCCCGAGAAGATCGGAAGAGCACACGTCTGAACTC1290731.2682577410857023Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AAACCGTTACCATTACTGAGATCGGAAGAGCACACGTCTGAACTCCAGTC965640.9488277216009527Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CAACGGAATCCCAAAAGCAGCTAGATCGGAAGAGCACACGTCTGAACTCC964550.9477566990495411Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TATTGCACTTGTCCCGGCCTGTAAGATCGGAAGAGCACACGTCTGAACTC959010.9423131532377798Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCCTGTACTGAGCTGCCCCGAGATCGGAAGAGCACACGTCTGAACTCCAG913530.8976249829275075Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AAATCGTTACCATTACTGAGTAGATCGGAAGAGCACACGTCTGAACTCCA834550.8200200644775227Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TAACCGTTACCATTACTGAGTAGATCGGAAGAGCACACGTCTGAACTCCA809730.7956321931692344Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AAACCGTTACCATTACTGAGAGATCGGAAGAGCACACGTCTGAACTCCAG807360.7933034560620368Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GAACCGTTACCATTACTGAGTAGATCGGAAGAGCACACGTCTGAACTCCA480300.47193773526877264Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AAACCGTTACCATTACTGAGAAGATCGGAAGAGCACACGTCTGAACTCCA458490.45050745834557476Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TATTGCACTTGTCCCGGCCTGTTAGATCGGAAGAGCACACGTCTGAACTC445240.43748814751419596Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AAACCGTTACCATTACTGAGTTAGATCGGAAGAGCACACGTCTGAACTCC436260.4286644938337596Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCTGTACTGAGCTGCCCCGAAAGATCGGAAGAGCACACGTCTGAACTCC415200.4079711590330926Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAAGCGTTACCATTACTGAGTAGATCGGAAGAGCACACGTCTGAACTCCA365170.3588122065127997Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATG349170.3430907745654744TruSeq Adapter, Index 12 (100% over 49bp)
TGACATGGGACTGCCTAAGCTAAGATCGGAAGAGCACACGTCTGAACTCC347180.3411354214670258Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCTGTACTGAGCTGCCCCAAGATCGGAAGAGCACACGTCTGAACTCCAG325380.319714970438795Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TATTGCACTTGTCCCGGCCTGCAGATCGGAAGAGCACACGTCTGAACTCC325290.3196265373840913Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTGAATTGAAGTGCTTGAATTAGATCGGAAGAGCACACGTCTGAACTCCA289110.28407644939320187Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CATTGCACTTGTCTCGGTCTGAAGATCGGAAGAGCACACGTCTGAACTCC278790.273936125787177Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGACCGTTACCATTACTGAGTAGATCGGAAGAGCACACGTCTGAACTCCA277270.2724425897521811Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CCCATAAAGTAGAAAGCACTAGATCGGAAGAGCACACGTCTGAACTCCAG275180.27038897770406173Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AAACCGTTACCATTACTGAGGAGATCGGAAGAGCACACGTCTGAACTCCA267520.2628623421592797Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GATGGATATGTCTTCAAGGACAGATCGGAAGAGCACACGTCTGAACTCCA264260.25965910040001217Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCCGTC253060.24865409803688446TruSeq Adapter, Index 12 (100% over 50bp)
AAGCTGCCAGTTGAAGAACTGTAGATCGGAAGAGCACACGTCTGAACTCC218200.21440102818164938Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCTGTACTGAGCTGCCCCGAGCAGATCGGAAGAGCACACGTCTGAACTC213010.20930138869373568Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AATGACACGATCACTCCCGTAGATCGGAAGAGCACACGTCTGAACTCCAG209290.20564615576598258Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCGCTCTGATACCAAATTGATGAGATCGGAAGAGCACACGTCTGAACTCC201260.19775596210741866Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TAATCGTTACCATTACTGAGTAGATCGGAAGAGCACACGTCTGAACTCCA190380.1870653883832374Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACG186310.18306624913163652No Hit
TGTAAACATCCTACACTCTCAGCTAGATCGGAAGAGCACACGTCTGAACT181280.17812382396319612Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AAACCGTTACCATTACTAAGATCGGAAGAGCACACGTCTGAACTCCAGTC180600.17745566310543479Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CAACGGAATCCCAAAAGCAGCCAGATCGGAAGAGCACACGTCTGAACTCC176770.17369234533304378Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAGCCGTTACCATTACTGAGTAGATCGGAAGAGCACACGTCTGAACTCCA174680.17163873328492443Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AAACCGTTACCATTACTGGGTAGATCGGAAGAGCACACGTCTGAACTCCA169230.16628362052786674Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGATCTCTTCGTACTCTTCTTGAGATCGGAAGAGCACACGTCTGAACTCC163050.16021121743821232Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCCTGTACTGAGCTGCCCCGAGAGATCGGAAGAGCACACGTCTGAACTCC153920.15124017533326978Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCGACCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAA152050.14940273297442613TruSeq Adapter, Index 12 (100% over 40bp)
TGAGGGGCAGAGAGCGAGACTAGATCGGAAGAGCACACGTCTGAACTCCA151770.14912760791534796Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TATTGCACTTGTCCCGGCCTGAGATCGGAAGAGCACACGTCTGAACTCCA145170.14264251723707624Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AAACCGTTACCATTACCGAGTAGATCGGAAGAGCACACGTCTGAACTCCA124350.12218500391561915Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AAGCTGCCAGTTGAAGAACTAGATCGGAAGAGCACACGTCTGAACTCCAG121420.1193060166902652Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TAACCGTTACCATTACTGAGCAGATCGGAAGAGCACACGTCTGAACTCCA121300.11918810595066025Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGTTTGTTGTACTCGGTCTAGTAGATCGGAAGAGCACACGTCTGAACTCC119580.11749805201632278Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCAGGCTCAGTCCCCTCCCGATAGATCGGAAGAGCACACGTCTGAACTCC119010.11693797600319931Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GATTGCACTTGTCCCGGCCTGTAGATCGGAAGAGCACACGTCTGAACTCC118100.11604381956119517Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TATTGCACTTGTCCCGGCCTGAAGATCGGAAGAGCACACGTCTGAACTCC116320.11429481025705523Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CATTGCACTTGTCTCGGTCTGAGATCGGAAGAGCACACGTCTGAACTCCA107880.10600175490484112Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AAACCGTTACCGTTACTGAGTAGATCGGAAGAGCACACGTCTGAACTCCA107720.10584454058536787Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AAACCGCTACCATTACTGAGTAGATCGGAAGAGCACACGTCTGAACTCCA107500.10562837089609216Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CAACGGAATCCCACAAGCAGCTAGATCGGAAGAGCACACGTCTGAACTCC107030.10516655383263945Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCCTGTACTGAGCTGCCCCGAAGATCGGAAGAGCACACGTCTGAACTCCA105290.10345684810836783Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCTGC1100.044.0181661
GTCATAC254.4344968E-544.018161
AAATCGT110950.044.018161
GATCGAT302.5225127E-644.018161
GCGAATT454.783942E-1044.018161
TATGGCG207.843444E-444.018161
TTGTAGG502.7284841E-1144.018161
CGACATG600.044.018161
TAGCCCG207.843444E-444.018161
TATGACA1500.044.018161
TGGCATG351.4428952E-744.018161
AATGCGT351.4428952E-744.018161
TCGTGTG302.5225127E-644.018161
AAGCCGT23150.044.018161
GTTAAAC750.044.018161
GATGGGT351.4428952E-744.018161
TAACATG700.044.018161
AGTGACA302.5225127E-644.018161
ATATAAT302.5225127E-644.018161
TCTAAGT2750.044.018161
Produced by FastQC (version 0.11.5)