FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005488312

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005488312
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7206875
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCGTTACCATTACTGAGTAGATCGGAAGAGCACACGTCTGAACTCCA252812235.079309686930884Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCCTGTACTGAGCTGCCCCGAGAGATCGGAAGAGCACACGTCTGAACTCC3446794.782641574885092Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAACCGTTACCATTACTGAGCAGATCGGAAGAGCACACGTCTGAACTCCA3329114.61935304830457Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCCTGTACTGAGCTGCCCCGAAGATCGGAAGAGCACACGTCTGAACTCCA1614112.239680860289654Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TATTGCACTTGTCCCGGCCTGTAGATCGGAAGAGCACACGTCTGAACTCC1312171.8207197988032262Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCTGTACTGAGCTGCCCCGAGAAGATCGGAAGAGCACACGTCTGAACTC1294311.7959379065128782Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TATTGCACTTGTCCCGGCCTGTAAGATCGGAAGAGCACACGTCTGAACTC1125861.5622027577833666Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCCTGTACTGAGCTGCCCCGAGTAGATCGGAAGAGCACACGTCTGAACTC1005631.3953759431098778Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCCTGTACTGAGCTGCCCCGAGATCGGAAGAGCACACGTCTGAACTCCAG869411.20636198074755Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AAACCGTTACCATTACTGAGATCGGAAGAGCACACGTCTGAACTCCAGTC778861.0807180643482786Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CAACGGAATCCCAAAAGCAGCTAGATCGGAAGAGCACACGTCTGAACTCC718600.9971034602376203Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAACCGTTACCATTACTGAGAGATCGGAAGAGCACACGTCTGAACTCCAG576050.7993062180209868Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TAACCGTTACCATTACTGAGTAGATCGGAAGAGCACACGTCTGAACTCCA504780.7004145347324603Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCCTGTACTGAGCTGCCCCGAAAGATCGGAAGAGCACACGTCTGAACTCC410500.5695950047697511Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CATTGCACTTGTCTCGGTCTGAAGATCGGAAGAGCACACGTCTGAACTCC405580.5627681900962623Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAATCGTTACCATTACTGAGTAGATCGGAAGAGCACACGTCTGAACTCCA375590.5211551469950568Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GAACCGTTACCATTACTGAGTAGATCGGAAGAGCACACGTCTGAACTCCA368880.5118445928367011Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AAACCGTTACCATTACTGAGAAGATCGGAAGAGCACACGTCTGAACTCCA359850.49931489029572457Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TATTGCACTTGTCCCGGCCTGTTAGATCGGAAGAGCACACGTCTGAACTC305140.4234012661521117Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AAAGCGTTACCATTACTGAGTAGATCGGAAGAGCACACGTCTGAACTCCA305070.4233041366750499Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCCTGTACTGAGCTGCCCCAAGATCGGAAGAGCACACGTCTGAACTCCAG275260.3819408550862891Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TATTGCACTTGTCCCGGCCTGCAGATCGGAAGAGCACACGTCTGAACTCC229100.3178909027838002Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAACCGTTACCATTACTGAGGAGATCGGAAGAGCACACGTCTGAACTCCA209820.29113866967305524Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGACCGTTACCATTACTGAGTAGATCGGAAGAGCACACGTCTGAACTCCA206460.28647645477408723Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCCTGTACTGAGCTGCCCCGAGAGATCGGAAGAGCACACGTCTGAACTCC203270.2820501257479837Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CATTGCACTTGTCTCGGTCTGAGATCGGAAGAGCACACGTCTGAACTCCA177280.24598733847888302Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TATTGCACTTGTCCCGGCCTGAGATCGGAAGAGCACACGTCTGAACTCCA170170.2361217587373168Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TAATCGTTACCATTACTGAGTAGATCGGAAGAGCACACGTCTGAACTCCA169550.23526146908334056Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGTAAACATCCTACACTCTCAGCTAGATCGGAAGAGCACACGTCTGAACT167480.23238921169022636Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AAACCGTTACCATTACTGAGTTAGATCGGAAGAGCACACGTCTGAACTCC153320.2127413060445755Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCTGTACTGAGCTGCCCCGAGCAGATCGGAAGAGCACACGTCTGAACTC149710.20773220015610094Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TATTGCACTTGTCCCGGCCTGAAGATCGGAAGAGCACACGTCTGAACTCC146060.20266759170930534Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAACCGTTACCATTACTAAGATCGGAAGAGCACACGTCTGAACTCCAGTC136860.1899020032954644Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
AATGACACGATCACTCCCGTAGATCGGAAGAGCACACGTCTGAACTCCAG134960.18726563177521463Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCTCTCGGCTCCTCGCGGCTCGAGATCGGAAGAGCACACGTCTGAACTCC132220.18346370653022287Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTCAAGTAATCCAGGATAGGCTAGATCGGAAGAGCACACGTCTGAACTCC131200.18204839129303615Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CAACGGAATCCCAAAAGCAGCCAGATCGGAAGAGCACACGTCTGAACTCC127620.1770809123233024Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATG126390.1753742086549302TruSeq Adapter, Index 11 (100% over 49bp)
GCCGGTACTGAGCTGCCCCGAGAGATCGGAAGAGCACACGTCTGAACTCC119750.16616078397363626Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAGCCGTTACCATTACTGAGTAGATCGGAAGAGCACACGTCTGAACTCCA119620.16598040065909286Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ATACCGTTACCATTACTGAGTAGATCGGAAGAGCACACGTCTGAACTCCA116390.16149856907466828Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CCCATAAAGTAGAAAGCACTAGATCGGAAGAGCACACGTCTGAACTCCAG110690.153589454513919Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGCGACCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACA110180.15288179689532563TruSeq Adapter, Index 11 (100% over 40bp)
GATGGATATGTCTTCAAGGACAGATCGGAAGAGCACACGTCTGAACTCCA109770.1523128956725349Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCGCTCTGATACCAAATTGATGAGATCGGAAGAGCACACGTCTGAACTCC99710.13835400225479141Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CAAAGAATTCTCCTTTTGGGAGATCGGAAGAGCACACGTCTGAACTCCAG96270.13358078223918132Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CTTCTCACTACTGCACTTGACTAGTAGATCGGAAGAGCACACGTCTGAAC95820.13295637845806954Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AAACCGTTACCATTACTGGGTAGATCGGAAGAGCACACGTCTGAACTCCA94650.1313329286271789Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCCTGTACTGAGCTGCCCCGAAGATCGGAAGAGCACACGTCTGAACTCCA94410.13099991327725263Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GATTGCACTTGTCCCGGCCTGTAGATCGGAAGAGCACACGTCTGAACTCC94070.13052814153152373Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCGGTACTGAGCTGCCCCGAGAGATCGGAAGAGCACACGTCTGAACTCC92520.12837741739658312Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGACATGGGACTGCCTAAGCTAAGATCGGAAGAGCACACGTCTGAACTCC91120.12643482785534646Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGAGGGGCAGAGAGCGAGACTAGATCGGAAGAGCACACGTCTGAACTCCA90600.12571329459717284Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CCCCCCACTGCTAAATTTGACTGGCAGATCGGAAGAGCACACGTCTGAAC88960.12343768970600989Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACG88770.1231740525539849No Hit
TCAGGCTCAGTCCCCTCCCGATAGATCGGAAGAGCACACGTCTGAACTCC87860.12191136935218107Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAACCGTTACCATTACCGAGTAGATCGGAAGAGCACACGTCTGAACTCCA81360.11289220362501085Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGCTCGGTCTGAGGCCCCTCAGTAGATCGGAAGAGCACACGTCTGAACTC79340.11008932442979794Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGTAAACATCCTACACTCTCAGCAGATCGGAAGAGCACACGTCTGAACTC78550.10899314890295723Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GATTGCACTTGTCCCGGCCTGTAAGATCGGAAGAGCACACGTCTGAACTC78390.10877113866967306Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CCCCCCACTGCTAAATTTGACTGGAGATCGGAAGAGCACACGTCTGAACT77900.10809123233024023Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GCCTGTACTGAGCTGCCCCGAGAAGATCGGAAGAGCACACGTCTGAACTC76190.10571849796201543Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CAACGGAATCCCAAAAGCAGCTGAGATCGGAAGAGCACACGTCTGAACTC75120.1042338045269274Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AAACCGCTACCATTACTGAGTAGATCGGAAGAGCACACGTCTGAACTCCA73140.10148642789003556Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TTGAATTGAAGTGCTTGAATTAGATCGGAAGAGCACACGTCTGAACTCCA73060.10137542277339345Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCCTGTACTGAGCTGCCCCTAGATCGGAAGAGCACACGTCTGAACTCCAG72370.1004180036423554Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATTACA5300.044.0181351
TTAGGGG207.8433205E-444.018131
GCGAATC502.7284841E-1144.018131
CTATACG2850.044.018131
TATGGCA14500.044.018131
GTCTACG1050.044.018131
AATGGCG2100.044.018131
TAGCCGT254.4343866E-544.018131
AAAACCG551.8189894E-1244.018131
ATCAAGT254.4343866E-544.018131
TATTGCG1600.044.018131
GACCAAC254.4343866E-544.018131
CCTCCGT254.4343866E-544.018131
AATGACC650.044.018131
TAACATG207.8433205E-444.018131
TCATGTA1300.044.018131
ACTAAAC207.8433205E-444.018131
CAATGGA2600.044.018131
GAACCGT51400.044.018131
GCATGCT950.044.018131
Produced by FastQC (version 0.11.5)