FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005488313

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005488313
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7907729
Sequences flagged as poor quality0
Sequence length40
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTGTACTGAGCTGCCCCGAGAGATCGGAAGAGCACACG86513910.94042297099458No Hit
AAACCGTTACCATTACTGAGTAGATCGGAAGAGCACACGT79358210.035523473300616No Hit
TCCTGTACTGAGCTGCCCCGAAGATCGGAAGAGCACACGT4927286.2309671967767235No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGA4013545.075464776296709No Hit
TCCTGTACTGAGCTGCCCCGAGAAGATCGGAAGAGCACAC3663704.633062159818578No Hit
TCCTGTACTGAGCTGCCCCGAGATCGGAAGAGCACACGTC3088923.9062036647942793No Hit
TATTGCACTTGTCCCGGCCTGTAAGATCGGAAGAGCACAC2976103.7635331205710263No Hit
TCCTGTACTGAGCTGCCCCGAGTAGATCGGAAGAGCACAC2949513.729907790213853No Hit
TCCTGTACTGAGCTGCCCCGAAAGATCGGAAGAGCACACG1580171.9982601831701616No Hit
AAACCGTTACCATTACTGAGCAGATCGGAAGAGCACACGT1443391.8252901686438672No Hit
TCCTGTACTGAGCTGCCCCAAGATCGGAAGAGCACACGTC896051.1331319017128685No Hit
TATTGCACTTGTCCCGGCCTGTAGATCGGAAGAGCACACG821681.0390846727296799No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACCAGATCGGA772810.9772843758302795No Hit
CATTGCACTTGTCTCGGTCTGAAGATCGGAAGAGCACACG712660.9012195536796974No Hit
TCGCTCTGATACCAAATTGATGAGATCGGAAGAGCACACG622020.7865975174414803No Hit
TCCTGTACTGAGCTGCCCCGAGCAGATCGGAAGAGCACAC571070.7221668825525002No Hit
TCTCTCGGCTCCTCGCGGCTCGAGATCGGAAGAGCACACG533390.6745172982028089No Hit
CTAGACTGAAGCTCCTTGAGGAGATCGGAAGAGCACACGT504740.6382869215674942No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAAGATCGGA492320.6225807687643317No Hit
TCAGTGCACTACAGAACTTTGTAGATCGGAAGAGCACACG450270.5694049454653796No Hit
TGAGGTAGTAGTTTGTGCTGTTAGATCGGAAGAGCACACG398410.5038235377059583No Hit
GATGGATATGTCTTCAAGGACAGATCGGAAGAGCACACGT362070.45786849802263074No Hit
TCCTGTACTGAGCTGCCCCTAGATCGGAAGAGCACACGTC358560.45342980266521526No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCC355540.44961075423803726TruSeq Adapter, Index 20 (100% over 39bp)
TGAGGGGCAGAGAGCGAGACTAGATCGGAAGAGCACACGT336460.42548246152593244No Hit
AAACCGTTACCATTACTGAGAAGATCGGAAGAGCACACGT328580.415517527219256No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAGATCGGAA301570.3813610709218791No Hit
TTGAATTGAAGTGCTTGAATTAGATCGGAAGAGCACACGT273180.34545948653526193No Hit
AGCTCGGTCTGAGGCCCCTCAAGATCGGAAGAGCACACGT266360.3368350129348135No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTATTAGAT236130.29860659109587595No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATTAGATCGGA233160.2948507719472936No Hit
AAGGGCTTCCTCTCTGCAGGAAGATCGGAAGAGCACACGT227380.287541467341635No Hit
AAACCGTTACCATTACTGAGATCGGAAGAGCACACGTCTG221120.27962516166145807Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCTGTACTGAGCTGCCCCCAGATCGGAAGAGCACACGTC211510.2674724943153717No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTAGATCGG207930.2629452779679223No Hit
AGCTCGGTCTGAGGCCCCTCAGATCGGAAGAGCACACGTC203700.2575960809987292No Hit
CAACGGAATCCCAAAAGCAGCTAGATCGGAAGAGCACACG197880.2502361929701941No Hit
CATTGCACTTGTCTCGGTCTGAGATCGGAAGAGCACACGT194400.245835435179936No Hit
TGACATGGGACTGCCTAAGCTAAGATCGGAAGAGCACACG192680.2436603479962452No Hit
CGCGACCTCAGATCAGACGTAGATCGGAAGAGCACACGTC191160.24173817792693705No Hit
TCCTGTACTGAGCTGCCCCGATAGATCGGAAGAGCACACG183860.23250670325197031No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATAGATCGGAA182510.23079951272988744No Hit
TATTGCACTTGTCCCGGCCTGTTAGATCGGAAGAGCACAC180460.22820711230746527No Hit
TCTCTCGGCTCCTCGCGGCTCAAGATCGGAAGAGCACACG176920.22373047938289234No Hit
TTTGGCAATGGTAGAACTCACACTAGATCGGAAGAGCACA175430.22184624688074162No Hit
CCCCCCACTGCTAAATTTGACTGGCAGATCGGAAGAGCAC171920.2174075515233261No Hit
AGCTCGGTCTGAGGCCCCTCAGTAGATCGGAAGAGCACAC160230.20262454618766018No Hit
TCCTGTACTGAGCTGCCCCGTAGATCGGAAGAGCACACGT160080.2024348583518732No Hit
TATTGCACTTGTCCCGGCCTGAAGATCGGAAGAGCACACG156150.1974650370542541No Hit
TGACTGTGCTGAGTCTGTTCAATCCAACCCTGAGCAGATC155070.19609928463658782No Hit
TGATCTCTTCGTACTCTTCTTGAGATCGGAAGAGCACACG150280.19004191974712337No Hit
TGAGGGGCAGAGAGCGAGACTTTAGATCGGAAGAGCACAC149840.1894855020954815No Hit
TATTGCACTTGTCCCGGCCTGCAGATCGGAAGAGCACACG142030.17960908877883902No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTATTAAGA138950.17571416521734623No Hit
TCCTGTACTGAGCTGCCCCGAGGAGATCGGAAGAGCACAC127930.16177843221486218No Hit
CTAGACTGAAGCTCCTTGAGAAGATCGGAAGAGCACACGT124690.15768117496186326No Hit
TCGCTCTGATACCAAATTGATAAGATCGGAAGAGCACACG122080.15438060661916966No Hit
AAACCGTTACCATTACTGAGGAGATCGGAAGAGCACACGT119030.15052362062483426No Hit
TGTAAACATCCCCGACTGGAAGCTAGATCGGAAGAGCACA112620.14241762710887032No Hit
CGCGACCTCAGATCAGACGTGGCAGATCGGAAGAGCACAC109840.13890207921895148No Hit
CTAGACTGAAGCTCCTTGAGGAAGATCGGAAGAGCACACG109400.13834566156730965No Hit
GATGGATATGTCTTCAAGGATAGATCGGAAGAGCACACGT108730.13749838923412777No Hit
TGGAGAGAAAGGCAGTTCCTGAAGATCGGAAGAGCACACG100520.12711614168871999No Hit
TAAGATCCGGACTACAACAAAGAGATCGGAAGAGCACACG97820.1237017606445542No Hit
AAAAGCTGGGTTGAGAGGGCGAAGATCGGAAGAGCACACG96190.12164048616233561No Hit
CTTCTCACTACTGCACTTGACTAGTAGATCGGAAGAGCAC91350.11551989199427547No Hit
CAACGGAATCCCAAAAGCAGCTGAGATCGGAAGAGCACAC91160.11527962073561195No Hit
TCCTGTACTGAGCTGCCCCGAGTTAGATCGGAAGAGCACA91020.1151025787555441No Hit
TGTTTGTTGTACTCGGTCTAGTAGATCGGAAGAGCACACG85920.10865319233878652No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACGAGATCGGA84760.10718627307536716No Hit
TCAGTGCACTACAGAACTTTGCAGATCGGAAGAGCACACG84370.10669308470232099No Hit
TCTCTCGGCTCCTCGCGGCTCGTTAGATCGGAAGAGCACA82880.10480885220017025No Hit
CCCCCCACTGCTAAATTTGACTGGTAGATCGGAAGAGCAC80580.10190030538476977No Hit
TCTAGCAGCTGTTGAGCAGGTAGATCGGAAGAGCACACGT80370.10163474241466797No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)