FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005488316

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005488316
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4779111
Sequences flagged as poor quality0
Sequence length40
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTGTACTGAGCTGCCCCGAGAGATCGGAAGAGCACACG50147810.493123093395404No Hit
TCCTGTACTGAGCTGCCCCGAAGATCGGAAGAGCACACGT3261216.823884191013768No Hit
TCCTGTACTGAGCTGCCCCGAGAAGATCGGAAGAGCACAC2489645.209420747917343No Hit
TCCTGTACTGAGCTGCCCCGAGTAGATCGGAAGAGCACAC1771143.706003062075771No Hit
TATTGCACTTGTCCCGGCCTGTAGATCGGAAGAGCACACG1474283.084841511318737No Hit
AAACCGTTACCATTACTGAGTAGATCGGAAGAGCACACGT1464883.0651725812604056No Hit
TCCTGTACTGAGCTGCCCCGAGATCGGAAGAGCACACGTC1219782.5523156921862666No Hit
TATTGCACTTGTCCCGGCCTGTAAGATCGGAAGAGCACAC884611.8509927892446942No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTC813601.7024086697295795TruSeq Adapter, Index 8 (100% over 40bp)
CATTGCACTTGTCTCGGTCTGAAGATCGGAAGAGCACACG566981.1863712728162203No Hit
TCCTGTACTGAGCTGCCCCGAAAGATCGGAAGAGCACACG547631.1458825710472094No Hit
TGACATGGGACTGCCTAAGCTAAGATCGGAAGAGCACACG515421.0784850990069075No Hit
TTGAATTGAAGTGCTTGAATTAGATCGGAAGAGCACACGT359740.7527341382110606No Hit
TCGCTCTGATACCAAATTGATGAGATCGGAAGAGCACACG321380.672468164058127No Hit
GATGGATATGTCTTCAAGGACAGATCGGAAGAGCACACGT291420.6097786805956171No Hit
TCTCTCGGCTCCTCGCGGCTCGAGATCGGAAGAGCACACG289760.6063052312448906No Hit
TCCTGTACTGAGCTGCCCCAAGATCGGAAGAGCACACGTC286300.5990653910319303No Hit
TCCTGTACTGAGCTGCCCCGAGCAGATCGGAAGAGCACAC279870.5856110058962849No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGA275730.5769483069131476TruSeq Adapter, Index 8 (100% over 39bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGA263500.5513577734436383No Hit
TATTGCACTTGTCCCGGCCTGCAGATCGGAAGAGCACACG257260.5383009517878953No Hit
TGAGGGGCAGAGAGCGAGACTAGATCGGAAGAGCACACGT219480.4592485924683482No Hit
AAACCGTTACCATTACTGAGCAGATCGGAAGAGCACACGT211950.4434925240280043No Hit
TGATTGGAAATTTCGTTGACTAGATCGGAAGAGCACACGT211230.44198596768311094No Hit
CGCGACCTCAGATCGGAAGAGCACACGTCTGAACTCCAGT200440.41940854690338847Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CAACGGAATCCCAAAAGCAGCTAGATCGGAAGAGCACACG162350.3397075313797901No Hit
ATTGCACTTGTCCCGGCCTGTAGATCGGAAGAGCACACGT162100.3391844215378132No Hit
TGTAAACATCCCCGACTGGAAGCTAGATCGGAAGAGCACA158810.33230029601739736No Hit
TATTGCACTTGTCCCGGCCTGTTAGATCGGAAGAGCACAC152160.3183855742208122No Hit
CATTGCACTTGTCTCGGTCTGAGATCGGAAGAGCACACGT147270.3081535457117443No Hit
TATTGCACTTGTCCCGGCCTGAAGATCGGAAGAGCACACG135460.28344183677675616No Hit
TATTGCACTTGTCCCGGCCTGAGATCGGAAGAGCACACGT127660.26712080970707736No Hit
TGTTTGTTGTACTCGGTCTAGTAGATCGGAAGAGCACACG125380.2623500479482481No Hit
TGATCTCTTCGTACTCTTCTTGAGATCGGAAGAGCACACG122220.25573793954566026No Hit
AGCTCGGTCTGAGGCCCCTCAAGATCGGAAGAGCACACGT112900.23623640463676193No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAGATCGGAA112400.23519018495280816No Hit
ACCACAGGGTAGAACCACGGAAGATCGGAAGAGCACACGT110910.23207245029462595No Hit
AGCTCGGTCTGAGGCCCCTCAGATCGGAAGAGCACACGTC106250.22232168284017673No Hit
AGCTCGGTCTGAGGCCCCTCAGTAGATCGGAAGAGCACAC104820.219329494544069No Hit
CAACGGAATCCCAAAAGCAGCTGAGATCGGAAGAGCACAC99100.2073607413596378No Hit
TCCTGTACTGAGCTGCCCCTAGATCGGAAGAGCACACGTC97000.20296661868703195No Hit
CTAGACTGAAGCTCCTTGAGGAGATCGGAAGAGCACACGT91720.19191853882448012No Hit
TTATGTCTTGTTGATCTCAATAGATCGGAAGAGCACACGT89950.18821492114328375No Hit
AAACCGTTACCCTTACTGAGTAGATCGGAAGAGCACACGT87020.18208407379531466No Hit
CGCGACCTCAGATCAGACGTAGATCGGAAGAGCACACGTC86480.18095415653664457No Hit
TGGAGAGAAAGGCAGTTCCTGAAGATCGGAAGAGCACACG86280.1805356686630631No Hit
TCTAGCAGCTGTTGAGCAGGTAGATCGGAAGAGCACACGT84800.1774388583985599No Hit
TCCTGTACTGAGCTGCCCCGTAGATCGGAAGAGCACACGT80740.16894355456485527No Hit
TCCTGTACTGAGCTGCCCCGATAGATCGGAAGAGCACACG80710.16888078138381804No Hit
AAACCGTTACCATTACTGAGATCGGAAGAGCACACGTCTG76600.16028085558171803Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCTCTCGGCTCCTCGCGGCTCAAGATCGGAAGAGCACACG73810.15444294974525596No Hit
CGCGACCTCAGATCAGAAGATCGGAAGAGCACACGTCTGA70630.14778899255530997Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGAGGTAGTAGTTTGTGCTGTTAGATCGGAAGAGCACACG68790.14393890411836008No Hit
AAACCGTTACCATTACTGAGAGATCGGAAGAGCACACGTC68340.1429973064028017No Hit
AAACCGTTACCATTACTGAGTTAGATCGGAAGAGCACACG67340.14090486703489416No Hit
TCGCTCTGATACCCAATTGATGAGATCGGAAGAGCACACG67310.14084209385385693No Hit
TGAGGGGCAGAGAGCGAGACTTTAGATCGGAAGAGCACAC65670.13741049329048854No Hit
TCCTGTACTGAGCTGCCCCGAGGAGATCGGAAGAGCACAC65110.13623872724446032No Hit
TACCACAGGGTAGAACCACGGAAGATCGGAAGAGCACACG65100.13621780285078122No Hit
GGCTGGTCCGATGGTCGTGGGTTATCAGAACTAGATCGGA64700.1353808271036182No Hit
AGATCGGAAGAGCCCACGTCTGAACTCCAGTCACACTTGA63240.13232586562647322TruSeq Adapter, Index 8 (97% over 39bp)
TGACATGGGACTGCCTAAGCTAGATCGGAAGAGCACACGT63030.13188645335921262No Hit
GATGGATATGTCTTCCAGGACAGATCGGAAGAGCACACGT62300.1303589726206401No Hit
TCGCTCTGATACCAACTTGATGAGATCGGAAGAGCACACG55880.11692551187867367No Hit
AGATCGGAAGAGCACCCGTCTGAACTCCAGTCACACTTGA54130.11326374298483545TruSeq Adapter, Index 8 (97% over 39bp)
TGAGGGGCAGAGAGCGAGACTTAGATCGGAAGAGCACACG53610.11217567451352355No Hit
AAGGGCTTCCTCTCTGCAGGAAGATCGGAAGAGCACACGT52570.1099995375708997No Hit
CGCGACCTTAGATCGGAAGAGCACACGTCTGAACTCCAGT49820.10424532930915394Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CAAAGAATTCTCCTTTTGGGCAGATCGGAAGAGCACACGT48830.10217381433492546No Hit
CAAAGAATTCTCCTTTTGGGCTAGATCGGAAGAGCACACG48570.10162978009926951No Hit
TCCTGTACTGAGCTGCCCCGGAGATCGGAAGAGCACACGT47930.10029061890380869No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAAACA252.0289047E-434.0004962
CGCTCCG351.0921831E-634.0004922
TATCGCA1700.033.9997831
TAAAGTC301.484729E-533.999781
AGCGACC301.484729E-533.999781
CATAACG650.033.999783
GGCACGT301.4848352E-533.9994244
GTTTACG1650.033.9994244
GTCAAGT200.002790094633.9994244
ATTCGCG252.0295283E-433.9987145
CTTGCGT252.0297778E-433.99800517
GCGAAAA200.002790667133.99819
CGGCTAT301.4852609E-533.99816
TAAGCGA750.033.99816
GCAAGGC1100.033.9976467
GTTACCG700.033.9976466
ACCGAGT750.033.9972915
CTAAGCG800.033.9972915
CATCGCG200.002791096533.99693711
TTACCGA850.033.99693713
Produced by FastQC (version 0.11.5)