FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005488319

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005488319
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9850334
Sequences flagged as poor quality0
Sequence length40
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGA117798111.95879246328094No Hit
TCCTGTACTGAGCTGCCCCGAGAGATCGGAAGAGCACACG8862088.99673046619536No Hit
TCCTGTACTGAGCTGCCCCGAAGATCGGAAGAGCACACGT4783214.855886104978775No Hit
AAACCGTTACCATTACTGAGTAGATCGGAAGAGCACACGT4529484.5983009307095575No Hit
TATTGCACTTGTCCCGGCCTGTAAGATCGGAAGAGCACAC3891873.951003082738108No Hit
TCCTGTACTGAGCTGCCCCGAGATCGGAAGAGCACACGTC3370233.4214372832433906No Hit
TCCTGTACTGAGCTGCCCCGAGAAGATCGGAAGAGCACAC3213763.2625898776630318No Hit
TCCTGTACTGAGCTGCCCCGAGTAGATCGGAAGAGCACAC2363512.3994211769875013No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACCAGATCGGA2217002.2506851036726268No Hit
TCCTGTACTGAGCTGCCCCGAAAGATCGGAAGAGCACACG1582301.606341470248623No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAAGATCGGA1549541.5730837147248002No Hit
TATTGCACTTGTCCCGGCCTGTAGATCGGAAGAGCACACG1258361.2774795250597593No Hit
TCCTGTACTGAGCTGCCCCAAGATCGGAAGAGCACACGTC885660.8991167203061338No Hit
TCAGTGCACTACAGAACTTTGTAGATCGGAAGAGCACACG875580.8888835647603421No Hit
CATTGCACTTGTCTCGGTCTGAAGATCGGAAGAGCACACG868680.8818787261426871No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACGAGATCGGA822810.8353117772453199No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAGATCGGAA817100.8295150194907097No Hit
AAACCGTTACCATTACTGAGCAGATCGGAAGAGCACACGT809880.8221853187922359No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTAGATCGG739830.7510709789129993No Hit
TGAGGTAGTAGTTTGTGCTGTTAGATCGGAAGAGCACACG734330.7454874118989265No Hit
CTAGACTGAAGCTCCTTGAGGAGATCGGAAGAGCACACGT704110.7148082491416027No Hit
TCGCTCTGATACCAAATTGATGAGATCGGAAGAGCACACG651170.6610638786461455No Hit
TCTCTCGGCTCCTCGCGGCTCGAGATCGGAAGAGCACACG601800.6109437507398227No Hit
TGAGGGGCAGAGAGCGAGACTAGATCGGAAGAGCACACGT576850.5856146603759832No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTATTAGAT548360.556691783243086No Hit
TCCTGTACTGAGCTGCCCCGAGCAGATCGGAAGAGCACAC455360.4622787410051273No Hit
AAGGGCTTCCTCTCTGCAGGAAGATCGGAAGAGCACACGT387830.39372269001233867No Hit
GATGGATATGTCTTCAAGGACAGATCGGAAGAGCACACGT357720.3631551985952964No Hit
TTTGGCAATGGTAGAACTCACACTAGATCGGAAGAGCACA356640.36205878907253297No Hit
TTGAATTGAAGTGCTTGAATTAGATCGGAAGAGCACACGT350020.3553382047755944No Hit
TCCTGTACTGAGCTGCCCCTAGATCGGAAGAGCACACGTC346870.35214034366753455No Hit
TCCTGTACTGAGCTGCCCCGAGGAGATCGGAAGAGCACAC311280.3160095891164706No Hit
AGCTCGGTCTGAGGCCCCTCAAGATCGGAAGAGCACACGT269070.2731582502684681No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACG266590.2706405691421225TruSeq Adapter, Index 1 (100% over 39bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTATTAAGA254820.25869173573200666No Hit
TATTGCACTTGTCCCGGCCTGTTAGATCGGAAGAGCACAC253260.2571080330880151No Hit
TGACATGGGACTGCCTAAGCTAAGATCGGAAGAGCACACG244320.24803219870514034No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATTAGATCGGA238100.2417176920092253No Hit
AAACCGTTACCATTACTGAGGAGATCGGAAGAGCACACGT233360.2369056724370971No Hit
CATTGCACTTGTCTCGGTCTGAGATCGGAAGAGCACACGT232560.2360935172350501No Hit
TCCTGTACTGAGCTGCCCCCAGATCGGAAGAGCACACGTC228910.23238805912571087No Hit
TATTGCACTTGTCCCGGCCTGCAGATCGGAAGAGCACACG218860.22218535939999595No Hit
TCCTGTACTGAGCTGCCCCGGAGATCGGAAGAGCACACGT218000.2213122925577955No Hit
AAACCGTTACCATTACTGAGAAGATCGGAAGAGCACACGT213020.2162566264250532No Hit
AGCTCGGTCTGAGGCCCCTCAGATCGGAAGAGCACACGTC198970.2019931506891035No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAATAGATCGGAA196150.199130303601888No Hit
TGAGGGGCAGAGAGCGAGACTTTAGATCGGAAGAGCACAC196090.19906939196173448No Hit
TCTCTCGGCTCCTCGCGGCTCAAGATCGGAAGAGCACACG195300.19826738869971314No Hit
TATTGCACTTGTCCCGGCCTGTGAGATCGGAAGAGCACAC186950.18979051877834802No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATAGATCGGAA186610.18944535281747807No Hit
TATTGCACTTGTCCCGGCCTGAAGATCGGAAGAGCACACG181810.18457242160519635No Hit
CAACGGAATCCCAAAAGCAGCTAGATCGGAAGAGCACACG179130.18185170167833903No Hit
TCCTGTACTGAGCTGCCCCGATAGATCGGAAGAGCACACG167050.16958815812743No Hit
CCCCCCACTGCTAAATTTGACTGGCAGATCGGAAGAGCAC166940.16947648678714855No Hit
TGGAGAGAAAGGCAGTTCCTGAAGATCGGAAGAGCACACG166280.1688064587454598No Hit
TCAGTGCACTACAGAACTTTGCAGATCGGAAGAGCACACG165280.1677912647429011No Hit
TCCTGTACTGAGCTGCCCCGTAGATCGGAAGAGCACACGT161770.1642279337939201No Hit
TGACTGTGCTGAGTCTGTTCAATCCAACCCTGAGCAGATC160030.16246149622946796No Hit
AAACCGTTACCATTACTGAGATCGGAAGAGCACACGTCTG157860.1602585252439156Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTAGACTGAAGCTCCTTGAGAAGATCGGAAGAGCACACGT157150.1595377375020989No Hit
CGCGACCTCAGATCAGACGTAGATCGGAAGAGCACACGTC148570.1508273729601453No Hit
TGTAAACATCCCCGACTGGAAGCTAGATCGGAAGAGCACA141010.1431525063008016No Hit
TGATCTCTTCGTACTCTTCTTGAGATCGGAAGAGCACACG139010.1411221182956842No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTCAGATCGG132990.13501065040028085No Hit
TGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGA132620.13463502861933413No Hit
TGAGGTAGTAGTTTGTGCTGTCAGATCGGAAGAGCACACG132510.13452335727905268No Hit
AGCTCGGTCTGAGGCCCCTCAGTAGATCGGAAGAGCACAC131040.13303102209529138No Hit
CTAGACTGAAGCTCCTTGAGGAAGATCGGAAGAGCACACG128420.1303712138085876No Hit
AAAAGCTGGGTTGAGAGGGCGAAGATCGGAAGAGCACACG127570.12950829890641272No Hit
GATGGATATGTCTTCAAGGATAGATCGGAAGAGCACACGT122790.12465567157418216No Hit
TCGCTCTGATACCAAATTGATAAGATCGGAAGAGCACACG121690.12353895817136759No Hit
TAAGATCCGGACTACAACAAAGAGATCGGAAGAGCACACG116560.11833101293824148No Hit
CTTCTCACTACTGCACTTGACTAGTAGATCGGAAGAGCAC114310.11604682643248442No Hit
TGAGGGGCAGAGAGCGAGACCAGATCGGAAGAGCACACGT107850.10948867317595525No Hit
TGAGGTAGTAGGTTGTGTGGTTAGATCGGAAGAGCACACG99920.1014381847356648No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATTGCG650.034.0079961
TCGAAAC2800.034.0079961
AAATCGT1850.034.007991
CTATACG2400.034.007991
TATGGAT850.034.007991
TTCAAGT6950.034.007991
GTCTACG4450.034.007991
AATGGCG3700.034.007991
AAGCCGT1600.034.007991
TCTGTAT200.002786793734.007991
AAACCGC1700.034.007991
CGAATGT1700.034.007991
TCCCATA4750.034.007991
CAGAGTC200.002786793734.007991
TCAGTGC123050.034.007991
GTACATG252.026434E-434.0079881
TCATGTA1000.034.0079881
AAGATGT252.026434E-434.0079881
CCTATAC2850.034.0026442
CAAACGA200.002788944934.002642
Produced by FastQC (version 0.11.5)