FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005488320

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005488320
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15104010
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCGTTACCATTACTGAGTAGATCGGAAGAGCACACGTCTGAACTCCA391814125.94106465766376Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AAACCGTTACCATTACTGAGCAGATCGGAAGAGCACACGTCTGAACTCCA6056374.009776211747742Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCCTGTACTGAGCTGCCCCGAGAGATCGGAAGAGCACACGTCTGAACTCC5098083.3753155618938284Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TATTGCACTTGTCCCGGCCTGTAAGATCGGAAGAGCACACGTCTGAACTC4591473.039901324217873Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACG4067252.692827931125575No Hit
TCCTGTACTGAGCTGCCCCGAAGATCGGAAGAGCACACGTCTGAACTCCA3354162.2207082754844576Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TATTGCACTTGTCCCGGCCTGTAGATCGGAAGAGCACACGTCTGAACTCC2471231.6361416603934982Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCTGTACTGAGCTGCCCCGAGAAGATCGGAAGAGCACACGTCTGAACTC2447281.6202849441969382Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCCTGTACTGAGCTGCCCCGAGTAGATCGGAAGAGCACACGTCTGAACTC2276041.5069110785811186Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AAACCGTTACCATTACTGAGATCGGAAGAGCACACGTCTGAACTCCAGTC1725341.142305917435171Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TCCTGTACTGAGCTGCCCCGAGATCGGAAGAGCACACGTCTGAACTCCAG1623661.0749860467518229Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CATTGCACTTGTCTCGGTCTGAAGATCGGAAGAGCACACGTCTGAACTCC1416100.9375655868871908Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGAGGTAGTAGTTTGTGCTGTTAGATCGGAAGAGCACACGTCTGAACTCC1348810.89301450409527Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAACCGTTACCATTACTGAGAAGATCGGAAGAGCACACGTCTGAACTCCA1305860.8645783470747173Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCAGTGCACTACAGAACTTTGTAGATCGGAAGAGCACACGTCTGAACTCC1176900.7791970476714462Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAATCGTTACCATTACTGAGTAGATCGGAAGAGCACACGTCTGAACTCCA1044130.691293239345048Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TTCAAGTAATCCAGGATAGGCTAGATCGGAAGAGCACACGTCTGAACTCC978020.6475234060358805Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CAACGGAATCCCAAAAGCAGCTAGATCGGAAGAGCACACGTCTGAACTCC877350.5808722319437023Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCTGTACTGAGCTGCCCCGAAAGATCGGAAGAGCACACGTCTGAACTCC831020.5501982586081444Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACG813650.5386980013916834No Hit
TATTGCACTTGTCCCGGCCTGTTAGATCGGAAGAGCACACGTCTGAACTC799370.5292435584987033Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AAACCGTTACCATTACTGAGAGATCGGAAGAGCACACGTCTGAACTCCAG774150.5125460059944346Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TAACCGTTACCATTACTGAGTAGATCGGAAGAGCACACGTCTGAACTCCA759290.5027075591184064Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACCAGATCGGAAGAGCACACG706080.46747850405289726No Hit
CATTGCACTTGTCTCGGTCTGAGATCGGAAGAGCACACGTCTGAACTCCA606570.40159533792681545Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GAACCGTTACCATTACTGAGTAGATCGGAAGAGCACACGTCTGAACTCCA544410.3604407041573728Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCCTGTACTGAGCTGCCCCAAGATCGGAAGAGCACACGTCTGAACTCCAG466980.30917617242043666Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAAGATCGGAAGAGCACACG447890.29653714477148785No Hit
AAACCGTTACCATTACTGAGGAGATCGGAAGAGCACACGTCTGAACTCCA447580.2963319012633069Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATTAGATCGGAAGAGCACACG408820.270669841982361No Hit
TATTGCACTTGTCCCGGCCTGCAGATCGGAAGAGCACACGTCTGAACTCC408290.27031894179095484Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCTGTACTGAGCTGCCCCGAGCAGATCGGAAGAGCACACGTCTGAACTC377010.24960920973966516Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCGCTCTGATACCAAATTGATGAGATCGGAAGAGCACACGTCTGAACTCC371920.24623924375050069Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAACCGTTACCATTACTAAGATCGGAAGAGCACACGTCTGAACTCCAGTC360640.23877102835604586Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TGAGGTAGTAGTTTGTACAGTTAGATCGGAAGAGCACACGTCTGAACTCC339950.2250726793745502Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTATTAGATCGGAAGAGCA338810.2243179129251106No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTAGATCGGAAGAGCACAC337530.22347045585907319No Hit
AATTGCACGGTATCCATCTGTAAGATCGGAAGAGCACACGTCTGAACTCC335510.22213306267673286Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TATTGCACTTGTCCCGGCCTGAAGATCGGAAGAGCACACGTCTGAACTCC324920.2151216796069388Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TATTGCACTTGTCCCGGCCTGAGATCGGAAGAGCACACGTCTGAACTCCA310680.2056937197472724Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CCCATAAAGTAGAAAGCACTAGATCGGAAGAGCACACGTCTGAACTCCAG306400.20286003518270976Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TTTGGCAATGGTAGAACTCACACTAGATCGGAAGAGCACACGTCTGAACT304040.20129753621720325Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGACCGTTACCATTACTGAGTAGATCGGAAGAGCACACGTCTGAACTCCA303930.20122470787559069Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AAAGCGTTACCATTACTGAGTAGATCGGAAGAGCACACGTCTGAACTCCA301320.19949668995187372Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATG297970.19727873591185388TruSeq Adapter, Index 8 (100% over 49bp)
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATAGATCGGAAGAGCACACGT293730.19447153438060488No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAGATCGGAAGAGCACACGT276760.18323610749728053No Hit
TGAGGTAGTAGGTTGTATAGTAGATCGGAAGAGCACACGTCTGAACTCCA273550.18111084407385852Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GATGGATATGTCTTCAAGGACAGATCGGAAGAGCACACGTCTGAACTCCA242160.16032828368095625Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGAGGTAGTAGATTGTATAGTTAGATCGGAAGAGCACACGTCTGAACTCC233840.15481981275171297Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGAGGTAGTAGGTTGTATAGTTAGATCGGAAGAGCACACGTCTGAACTCC232500.15393263113570504Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTATTAAGATCGGAAGAGC231080.15299248345306976No Hit
AAACCGTTACCATTACTGAGTTAGATCGGAAGAGCACACGTCTGAACTCC220140.14574937384178108Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTTGGCAATGGTAGAACTCACAAGATCGGAAGAGCACACGTCTGAACTCC209630.1387909568386144Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTAGACTGAAGCTCCTTGAGGAGATCGGAAGAGCACACGTCTGAACTCCA209620.13878433608028598Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGAGGTAGTAGTTTGTGCTGTCAGATCGGAAGAGCACACGTCTGAACTCC209070.138420194372223Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTGAATTGAAGTGCTTGAATTAGATCGGAAGAGCACACGTCTGAACTCCA205060.13576527028252763Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGAGGTAGTAGATTGTATAGTAGATCGGAAGAGCACACGTCTGAACTCCA198530.13144191509407105Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGACATGGGACTGCCTAAGCTAAGATCGGAAGAGCACACGTCTGAACTCC192960.12775415270514254Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCAGTGCACTACAGAACTTTGCAGATCGGAAGAGCACACGTCTGAACTCC192110.12719138824722706Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TAATCGTTACCATTACTGAGTAGATCGGAAGAGCACACGTCTGAACTCCA188220.12461591325747268Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AAACCGTTACCATTACGGAGTAGATCGGAAGAGCACACGTCTGAACTCCA186070.12319245021686293Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCTCTCGGCTCCTCGCGGCTCGAGATCGGAAGAGCACACGTCTGAACTCC182270.12067656205206431Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGAGGTAGTAGTTTGTACAGTAGATCGGAAGAGCACACGTCTGAACTCCA179460.11881612896177904Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGGAGAGAAAGGCAGTTCCTGAAGATCGGAAGAGCACACGTCTGAACTCC174570.11557857813918292Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGAGGGGCAGAGAGCGAGACTAGATCGGAAGAGCACACGTCTGAACTCCA174010.11520781567279154Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TTCAAGTAATCCAGGATAGGCCAGATCGGAAGAGCACACGTCTGAACTCC171470.11352614305737352Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAACCGTTACCATTACCGAGTAGATCGGAAGAGCACACGTCTGAACTCCA168320.11144060418392202Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCCTGTACTGAGCTGCCCCTAGATCGGAAGAGCACACGTCTGAACTCCAG166200.11003700341829753Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ATACCGTTACCATTACTGAGTAGATCGGAAGAGCACACGTCTGAACTCCA161410.10686566017898559Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CAACGGAATCCCAAAAGCAGCCAGATCGGAAGAGCACACGTCTGAACTCC159720.10574675202148302Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GATTGCACTTGTCCCGGCCTGTAAGATCGGAAGAGCACACGTCTGAACTC159240.105428955621719Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCATTGCACTACAGAACTTTGTAGATCGGAAGAGCACACGTCTGAACTCC157110.1040187340977661Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGCTGGTCCGAAGGTAGTGAGTTATCTCAAAGATCGGAAGAGCACACGTC152480.10095332299170881No Hit
GCCTGTACTGAGCTGCCCCGAGAGATCGGAAGAGCACACGTCTGAACTCC152250.10080104555015522Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)