FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005488321

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005488321
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6782561
Sequences flagged as poor quality0
Sequence length40
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTGTACTGAGCTGCCCCGAGAGATCGGAAGAGCACACG73819610.883735509345216No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGA5143507.583418711604657No Hit
AAACCGTTACCATTACTGAGTAGATCGGAAGAGCACACGT4838797.134163629342957No Hit
TCCTGTACTGAGCTGCCCCGAAGATCGGAAGAGCACACGT4200166.192587136333901No Hit
TCCTGTACTGAGCTGCCCCGAGAAGATCGGAAGAGCACAC2488163.668466822487848No Hit
TCCTGTACTGAGCTGCCCCGAGATCGGAAGAGCACACGTC2076313.0612478089028614No Hit
TATTGCACTTGTCCCGGCCTGTAAGATCGGAAGAGCACAC1965322.897607555612106No Hit
TCCTGTACTGAGCTGCCCCGAGTAGATCGGAAGAGCACAC1240161.8284538834225008No Hit
TCCTGTACTGAGCTGCCCCGAAAGATCGGAAGAGCACACG1222211.8019889537300142No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACCAGATCGGA954661.4075214362244586No Hit
AAACCGTTACCATTACTGAGCAGATCGGAAGAGCACACGT865171.2755801237909987No Hit
CTAGACTGAAGCTCCTTGAGGAGATCGGAAGAGCACACGT760791.121685451852184No Hit
TCGCTCTGATACCAAATTGATGAGATCGGAAGAGCACACG650740.9594311057430962No Hit
CATTGCACTTGTCTCGGTCTGAAGATCGGAAGAGCACACG586400.8645701822659613No Hit
TCTCTCGGCTCCTCGCGGCTCGAGATCGGAAGAGCACACG580570.8559746090009365No Hit
TGAGGGGCAGAGAGCGAGACTAGATCGGAAGAGCACACGT558290.8231256600567248No Hit
TATTGCACTTGTCCCGGCCTGTAGATCGGAAGAGCACACG551250.8127460998876384No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAAGATCGGA549500.8101659535387887No Hit
TCCTGTACTGAGCTGCCCCAAGATCGGAAGAGCACACGTC548040.8080133742991771No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAGATCGGAA378620.5582257203436872No Hit
TGACTGTGCTGAGTCTGTTCAATCCAACCCTGAGCAGATC369810.5452365264389071No Hit
AAGGGCTTCCTCTCTGCAGGAAGATCGGAAGAGCACACGT334870.49372206162244614No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTAGATCGG326780.4817944136440498No Hit
GATGGATATGTCTTCAAGGACAGATCGGAAGAGCACACGT319810.4715180593289172No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGA287880.42444144623247765TruSeq Adapter, Index 8 (100% over 39bp)
TTGAATTGAAGTGCTTGAATTAGATCGGAAGAGCACACGT281970.41572792342007686No Hit
CGCGACCTCAGATCAGACGTAGATCGGAAGAGCACACGTC271040.3996130665098331No Hit
CAACGGAATCCCAAAAGCAGCTAGATCGGAAGAGCACACG239150.3525954281870815No Hit
TCCTGTACTGAGCTGCCCCGAGCAGATCGGAAGAGCACAC233170.34377869952072676No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTATTAGAT229240.3379844280058815No Hit
TGACATGGGACTGCCTAAGCTAAGATCGGAAGAGCACACG223620.3296984723027187No Hit
AGCTCGGTCTGAGGCCCCTCAAGATCGGAAGAGCACACGT217990.32139777290613386No Hit
CCCCCCACTGCTAAATTTGACTGGCAGATCGGAAGAGCAC212260.312949636575329No Hit
TCCTGTACTGAGCTGCCCCTAGATCGGAAGAGCACACGTC202780.29897261521127494No Hit
TCAGTGCACTACAGAACTTTGTAGATCGGAAGAGCACACG189570.27949619620081556No Hit
TCTCTCGGCTCCTCGCGGCTCAAGATCGGAAGAGCACACG176060.25957746638769635No Hit
AAACCGTTACCATTACTGAGAAGATCGGAAGAGCACACGT170580.2514979223924414No Hit
CTAGACTGAAGCTCCTTGAGAAGATCGGAAGAGCACACGT169190.24944854900678373No Hit
CTAGACTGAAGCTCCTTGAGGAAGATCGGAAGAGCACACG166380.2453055711552023No Hit
CATTGCACTTGTCTCGGTCTGAGATCGGAAGAGCACACGT161080.2374914136415434No Hit
AGCTCGGTCTGAGGCCCCTCAGATCGGAAGAGCACACGTC156750.23110739438981825No Hit
AAAAGCTGGGTTGAGAGGGCGAAGATCGGAAGAGCACACG154490.2277753196764467No Hit
TGAGGTAGTAGTTTGTGCTGTTAGATCGGAAGAGCACACG150820.22236438419057344No Hit
TTTGGCAATGGTAGAACTCACACTAGATCGGAAGAGCACA141130.20807774526465742No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATTAGATCGGA139070.20504054441972583No Hit
TGAGGGGCAGAGAGCGAGACTTTAGATCGGAAGAGCACAC136540.20131038998396034No Hit
CGCGACCTCAGATCAGACGTGGCAGATCGGAAGAGCACAC131210.1934520013900354No Hit
TGGAGAGAAAGGCAGTTCCTGAAGATCGGAAGAGCACACG125950.1856968186500645No Hit
AAACCGTTACCATTACTGAGATCGGAAGAGCACACGTCTG125590.18516604568687256Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCTGTACTGAGCTGCCCCCAGATCGGAAGAGCACACGTC124830.1840455249868007No Hit
TCCTGTACTGAGCTGCCCCGATAGATCGGAAGAGCACACG124320.18329359662227881No Hit
TGACTGTGCTGAGTCTGTTCAATCCAACCCTGAGTAGATC123420.181966664214299No Hit
TCGCTCTGATACCAAATTGATAAGATCGGAAGAGCACACG114180.16834349149237288No Hit
TCCTGTACTGAGCTGCCCCGTAGATCGGAAGAGCACACGT107260.15814085564435026No Hit
TGAGGGGCAGAGAGCGAGACCAGATCGGAAGAGCACACGT104990.15479403723755672No Hit
TATTGCACTTGTCCCGGCCTGAAGATCGGAAGAGCACACG104630.1542632642743648No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTATTAAGA104160.15357031068353091No Hit
TATTGCACTTGTCCCGGCCTGTTAGATCGGAAGAGCACAC100860.14870489185427155No Hit
TGATCTCTTCGTACTCTTCTTGAGATCGGAAGAGCACACG98900.14581512794355997No Hit
CTTCTCACTACTGCACTTGACTAGTAGATCGGAAGAGCAC97420.14363306131710427No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACGAGATCGGA97300.1434561369960403No Hit
TATTGCACTTGTCCCGGCCTGCAGATCGGAAGAGCACACG96470.14223241044201446No Hit
TAAGATCCGGACTACAACAAAGAGATCGGAAGAGCACACG94440.13923944067734886No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATAGATCGGAA92320.1361137776718853No Hit
ACCACAGGGTAGAACCACGGAAGATCGGAAGAGCACACGT92320.1361137776718853No Hit
CCCCCCACTGCTAAATTTGACTGGTAGATCGGAAGAGCAC92040.13570095425606937No Hit
AGGGCTGGGTCGGTCGGGCTGGGGCGAGATCGGAAGAGCA89220.1315432327110659No Hit
CTAGACTGAAGCTCCTTGAGGTAGATCGGAAGAGCACACG86230.12713486837788854No Hit
GGGACTGACCTGAAATGAAGAGAATACTCATTGCTGATCA85160.12555729318173475No Hit
TGTAAACATCCCCGACTGGAAGCTAGATCGGAAGAGCACA83610.12327202070132506No Hit
GATGGATATGTCTTCAAGGATAGATCGGAAGAGCACACGT83540.12316881484737106No Hit
CCCCCACTGCTAAATTTGACTGGCAGATCGGAAGAGCACA82450.1215617522643733No Hit
CGCGACCTCAGATCAGACAGATCGGAAGAGCACACGTCTG82230.12123739100908934Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCTAGCAGCTGTTGAGCAGGTAGATCGGAAGAGCACACGT78070.11510401454553817No Hit
AGCTCGGTCTGAGGCCCCTCAGTAGATCGGAAGAGCACAC74980.11054821327814081No Hit
CAACGGAATCCCAAAAGCAGCTGAGATCGGAAGAGCACAC74050.10917704978989498No Hit
TGTTTGTTGTACTCGGTCTAGTAGATCGGAAGAGCACACG73580.1084840961990611No Hit
CGCGACCTCAGATCAGAAGATCGGAAGAGCACACGTCTGA73340.10813024755693315Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TACCACAGGGTAGAACCACGGAAGATCGGAAGAGCACACG70360.10373662691717773No Hit
TCGCTCTGATACCAAATTGCTGAGATCGGAAGAGCACACG70130.10339752196847178No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGG69570.10257187513683991No Hit
CTAGACTGAAGCTCCTTGAGGAAAGATCGGAAGAGCACAC68480.10096481255384211No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACCGT200.002787609134.0058141
AGCTACA1050.034.0058141
AATCCGT252.0271316E-434.005811
AAATCGT1250.034.005811
CAACGGG301.4830188E-534.005811
CTATACG1700.034.005811
TATGGAT950.034.005811
GTCTACG1750.034.005811
GCGCAGT2550.034.005811
AAAGTTC351.0909389E-634.005811
TATTGCG1250.034.005811
AAGCCGT2150.034.005811
AACATTC2300.034.005811
TATCGCA1500.034.005811
TCGAAAC3900.034.005811
TCGTTCG301.484142E-534.0020525
AGTCTTA351.091883E-634.0020523
TCATAAC200.00278912134.0020525
TACAACT252.0284491E-434.0020525
ATCCGTT200.002789221734.00182
Produced by FastQC (version 0.11.5)