FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005488323

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005488323
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6084428
Sequences flagged as poor quality0
Sequence length40
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCGTTACCATTACTGAGTAGATCGGAAGAGCACACGT112294818.45609809171873No Hit
TCCTGTACTGAGCTGCCCCGAGAGATCGGAAGAGCACACG4140896.805717809463766No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGA3183465.232143432381811No Hit
TCCTGTACTGAGCTGCCCCGAAGATCGGAAGAGCACACGT3156055.1870940045637814No Hit
AAACCGTTACCATTACTGAGCAGATCGGAAGAGCACACGT2095733.4444158103276097No Hit
TATTGCACTTGTCCCGGCCTGTAAGATCGGAAGAGCACAC1828583.005344134239077No Hit
TCCTGTACTGAGCTGCCCCGAGAAGATCGGAAGAGCACAC1698512.7915689034367737No Hit
TCCTGTACTGAGCTGCCCCGAGATCGGAAGAGCACACGTC1200271.9726915989473455No Hit
TCCTGTACTGAGCTGCCCCGAGTAGATCGGAAGAGCACAC1112731.828816118787173No Hit
TATTGCACTTGTCCCGGCCTGTAGATCGGAAGAGCACACG858781.4114391689736487No Hit
TCCTGTACTGAGCTGCCCCGAAAGATCGGAAGAGCACACG837921.3771549272996575No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACCAGATCGGA611891.005665610637516No Hit
CATTGCACTTGTCTCGGTCTGAAGATCGGAAGAGCACACG557030.9155010134066834No Hit
AAACCGTTACCATTACTGAGAAGATCGGAAGAGCACACGT516180.8483624097450081No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAAGATCGGA460630.7570637700043455No Hit
TCGCTCTGATACCAAATTGATGAGATCGGAAGAGCACACG423480.69600626385915No Hit
TCCTGTACTGAGCTGCCCCAAGATCGGAAGAGCACACGTC416560.6846329679634634No Hit
GATGGATATGTCTTCAAGGACAGATCGGAAGAGCACACGT319680.5254068254238525No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTAC290710.4777934754096852TruSeq Adapter, Index 11 (100% over 39bp)
AAACCGTTACCATTACTGAGATCGGAAGAGCACACGTCTG290360.47721823645542355Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGAGGTAGTAGTTTGTGCTGTTAGATCGGAAGAGCACACG278700.45805456157916574No Hit
TGAGGGGCAGAGAGCGAGACTAGATCGGAAGAGCACACGT266250.43759249020614593No Hit
TTGAATTGAAGTGCTTGAATTAGATCGGAAGAGCACACGT227910.3745791716164609No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAGATCGGAA224140.36838302630912884No Hit
CAACGGAATCCCAAAAGCAGCTAGATCGGAAGAGCACACG220680.36269637836128554No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTAGATCGG216830.3563687498644079No Hit
TCTCTCGGCTCCTCGCGGCTCGAGATCGGAAGAGCACACG212860.3498438965832121No Hit
TCCTGTACTGAGCTGCCCCGAGCAGATCGGAAGAGCACAC211420.34747719917139297No Hit
TATTGCACTTGTCCCGGCCTGTTAGATCGGAAGAGCACAC201640.3314033792494545No Hit
CTAGACTGAAGCTCCTTGAGGAGATCGGAAGAGCACACGT192530.3164307310399597No Hit
TGACATGGGACTGCCTAAGCTAAGATCGGAAGAGCACACG192470.3163321186478006No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATTAGATCGGA190140.31250267075228766No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTATTAGAT189800.31194386719671924No Hit
AAACCGTTACCATTACTGAGGAGATCGGAAGAGCACACGT183180.3010636332618284No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATAGATCGGAA176890.29072576748381274No Hit
TCAGTGCACTACAGAACTTTGTAGATCGGAAGAGCACACG160300.2634594410518129No Hit
TGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGA157500.2588575294177201No Hit
TCCTGTACTGAGCTGCCCCTAGATCGGAAGAGCACACGTC154900.25458432575749107No Hit
CATTGCACTTGTCTCGGTCTGAGATCGGAAGAGCACACGT151550.24907846719527293No Hit
TATTGCACTTGTCCCGGCCTGCAGATCGGAAGAGCACACG150040.24659672199260144No Hit
TATTGCACTTGTCCCGGCCTGAAGATCGGAAGAGCACACG145430.2390200031950415No Hit
AAGGGCTTCCTCTCTGCAGGAAGATCGGAAGAGCACACGT134980.22184501156065944No Hit
CCCCCCACTGCTAAATTTGACTGGCAGATCGGAAGAGCAC132040.21701300434486198No Hit
AGCTCGGTCTGAGGCCCCTCAAGATCGGAAGAGCACACGT124310.20430844115502722No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTATTAAGA119460.19633727278883076No Hit
TGACTGTGCTGAGTCTGTTCAATCCAACCCTGAGCAGATC115140.1892371805533733No Hit
TCCTGTACTGAGCTGCCCCGTAGATCGGAAGAGCACACGT105860.1739851305660943No Hit
GATGGATATGTCTTCAAGGATAGATCGGAAGAGCACACGT103010.16930104193853554No Hit
TGATCTCTTCGTACTCTTCTTGAGATCGGAAGAGCACACG101120.1661947515855229No Hit
TCCTGTACTGAGCTGCCCCGATAGATCGGAAGAGCACACG99480.16349934619983997No Hit
TTTGGCAATGGTAGAACTCACACTAGATCGGAAGAGCACA95300.15662934954608715No Hit
TGAGGGGCAGAGAGCGAGACTTTAGATCGGAAGAGCACAC95280.15659647874870078No Hit
TCGCTCTGATACCAAATTGATAAGATCGGAAGAGCACACG94810.15582401501012091No Hit
TGTAAACATCCCCGACTGGAAGCTAGATCGGAAGAGCACA93090.1529971264348925No Hit
CGCGACCTCAGATCAGACGTAGATCGGAAGAGCACACGTC89310.1467845457288672No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGG87540.14387548016017282No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAAAGATCGGAAG86140.14157452434312642No Hit
AAACCGTTACCATTACTGAGAGATCGGAAGAGCACACGTC85510.14053909422545555No Hit
GAACCGTTACCATTACTGAGTAGATCGGAAGAGCACACGT83930.13794230123193174No Hit
TATTGCACTTGTCCCGGCCTGAGATCGGAAGAGCACACGT79960.13141744795073587No Hit
TCTCTCGGCTCCTCGCGGCTCAAGATCGGAAGAGCACACG78560.12911649213368948No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACGAGATCGGA77570.1274893876630638No Hit
TGTTTGTTGTACTCGGTCTAGTAGATCGGAAGAGCACACG77320.1270785026957341No Hit
CTTCTCACTACTGCACTTGACTAGTAGATCGGAAGAGCAC76650.12597733098329045No Hit
AAACCGTTACCATTACTAAGATCGGAAGAGCACACGTCTG75170.12354489197669856Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCTAGCAGCTGTTGAGCAGGTAGATCGGAAGAGCACACGT74940.12316687780675523No Hit
TCGCTCTGATACCCAATTGATGAGATCGGAAGAGCACACG72890.11979762107465156No Hit
AGCTCGGTCTGAGGCCCCTCAGATCGGAAGAGCACACGTC72500.1191566405256172No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGG72350.11891010954521936No Hit
TAAGATCCGGACTACAACAAAGAGATCGGAAGAGCACACG69260.11383157134902411No Hit
CCCCCCACTGCTAAATTTGACTGGTAGATCGGAAGAGCAC66740.10968985087834059No Hit
AAGCTGCCAGTTGAAGAACTAGATCGGAAGAGCACACGTC66180.10876946855152203No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)