FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005488324

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005488324
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5979840
Sequences flagged as poor quality0
Sequence length40
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTGTACTGAGCTGCCCCGAGAGATCGGAAGAGCACACG94951915.878668994488146No Hit
TCCTGTACTGAGCTGCCCCGAAGATCGGAAGAGCACACGT4557127.620805907850377No Hit
TCCTGTACTGAGCTGCCCCGAGATCGGAAGAGCACACGTC3228985.39977658265104No Hit
TCCTGTACTGAGCTGCCCCGAGAAGATCGGAAGAGCACAC2951194.935232380799486No Hit
AAACCGTTACCATTACTGAGTAGATCGGAAGAGCACACGT2813304.704640926847541No Hit
TCCTGTACTGAGCTGCCCCGAGTAGATCGGAAGAGCACAC2267123.7912720072777866No Hit
TATTGCACTTGTCCCGGCCTGTAGATCGGAAGAGCACACG1438112.404930566704126No Hit
TATTGCACTTGTCCCGGCCTGTAAGATCGGAAGAGCACAC1256452.101143174399315No Hit
TCCTGTACTGAGCTGCCCCGAAAGATCGGAAGAGCACACG889371.4872805961363513No Hit
TGAGGGGCAGAGAGCGAGACTAGATCGGAAGAGCACACGT703401.176285653127843No Hit
CATTGCACTTGTCTCGGTCTGAAGATCGGAAGAGCACACG645651.0797111628404772No Hit
TCCTGTACTGAGCTGCCCCAAGATCGGAAGAGCACACGTC597200.9986889281318565No Hit
TCCTGTACTGAGCTGCCCCGAGGAGATCGGAAGAGCACAC515380.861862524749826No Hit
TCCTGTACTGAGCTGCCCCGGAGATCGGAAGAGCACACGT440380.7364411087922085No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGA432910.7239491357628297No Hit
TCTCTCGGCTCCTCGCGGCTCGAGATCGGAAGAGCACACG396120.6624257505217531No Hit
AAACCGTTACCATTACTGAGCAGATCGGAAGAGCACACGT368890.6168894151014074No Hit
TCCTGTACTGAGCTGCCCCGAGCAGATCGGAAGAGCACAC331540.5544295499545138No Hit
TTGAATTGAAGTGCTTGAATTAGATCGGAAGAGCACACGT321520.5376732487825762No Hit
CAACGGAATCCCAAAAGCAGCTAGATCGGAAGAGCACACG300220.5020535666506127No Hit
TATTGCACTTGTCCCGGCCTGCAGATCGGAAGAGCACACG250520.4189409750093648No Hit
AAACCGTTACCATTACTGAGGAGATCGGAAGAGCACACGT248610.41574690961631083No Hit
TGAGGTAGTAGTTTGTGCTGTTAGATCGGAAGAGCACACG248010.41474353828864985No Hit
TGACATGGGACTGCCTAAGCTAAGATCGGAAGAGCACACG243830.40775338470594535No Hit
CATTGCACTTGTCTCGGTCTGAGATCGGAAGAGCACACGT238380.39863942847969175No Hit
TGTAAACATCCCCGACTGGAAGCTAGATCGGAAGAGCACA224210.37494314229143255No Hit
TATTGCACTTGTCCCGGCCTGTTAGATCGGAAGAGCACAC208680.3489725477604752No Hit
TCCTGTACTGAGCTGCCCCTAGATCGGAAGAGCACACGTC198940.3326844865414459No Hit
CTAGACTGAAGCTCCTTGAGGAGATCGGAAGAGCACACGT182520.30522555787445815No Hit
CGCGACCTCAGATCGGAAGAGCACACGTCTGAACTCCAGT180690.3021652753250923Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TATTGCACTTGTCCCGGCCTGAAGATCGGAAGAGCACACG169940.28418820570450043No Hit
TATTGCACTTGTCCCGGCCTGGAGATCGGAAGAGCACACG162700.2720808583507251No Hit
TATTGCACTTGTCCCGGCCTGAGATCGGAAGAGCACACGT161070.26935503291057955No Hit
TCGCTCTGATACCAAATTGATGAGATCGGAAGAGCACACG156500.2617126879648954No Hit
AGCTCGGTCTGAGGCCCCTCAGATCGGAAGAGCACACGTC152820.2555586771552416No Hit
AGCTCGGTCTGAGGCCCCTCAAGATCGGAAGAGCACACGT151030.2525652860277198No Hit
TGAGGGGCAGAGAGCGAGACTTTAGATCGGAAGAGCACAC144380.24144458714614436No Hit
AGCTCGGTCTGAGGCCCCTCAGTAGATCGGAAGAGCACAC142720.23866859313961575No Hit
ATTGCACTTGTCCCGGCCTGTAGATCGGAAGAGCACACGT134410.22477190025151175No Hit
AAACCGTTACCATTACTGAGATCGGAAGAGCACACGTCTG132390.22139388344838656Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCTGTACTGAGCTGCCCCGTAGATCGGAAGAGCACACGT130260.21783191523519024No Hit
TCCTGTACTGAGCTGCCCCCAGATCGGAAGAGCACACGTC126090.2108584845079467No Hit
GATGGATATGTCTTCAAGGACAGATCGGAAGAGCACACGT124740.20860089902070958No Hit
ACCACAGGGTAGAACCACGGAAGATCGGAAGAGCACACGT123470.2064770963771606No Hit
TGAGGGGCAGAGAGCGAGACTTAGATCGGAAGAGCACACG114880.19211216353614813No Hit
TCCTGTACTGAGCTGCCCCGATAGATCGGAAGAGCACACG113920.1905067694118906No Hit
AAGCTGCCAGTTGAAGAACTAGATCGGAAGAGCACACGTC110740.18518890137528762No Hit
TGAGGGGCAGAGAGCGAGACCAGATCGGAAGAGCACACGT107030.1789847219992508No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAGATCGGAA104490.17473711671215283No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACG98730.16510475196660782TruSeq Adapter, Index 1 (100% over 39bp)
TGAGGGGCAGAGCGCGAGACTAGATCGGAAGAGCACACGT95420.15956948680901162No Hit
TATTGCACTTGTCCCGGCCTGTGAGATCGGAAGAGCACAC93900.15702761277893723No Hit
TCTCTCGGCTCCTCGCGGCTCAAGATCGGAAGAGCACACG91550.15309774174559854No Hit
AAGGGCTTCCTCTCTGCAGGAAGATCGGAAGAGCACACGT90580.15147562476588003No Hit
AACCCGTAGATCCGAACTTGTAGATCGGAAGAGCACACGT85560.14308075132445014No Hit
AAACCGTTACCATTACTGAGAGATCGGAAGAGCACACGTC82570.13808061754160647No Hit
CGCGACCTCAGATCAGACGTAGATCGGAAGAGCACACGTC80820.1351541178359287No Hit
AAACCGTTACCATTACTGAGTTAGATCGGAAGAGCACACG79480.13291325520415262No Hit
CATTGCACTTGTCTCGGTCTGGAGATCGGAAGAGCACACG79100.13227778669663404No Hit
TGAGGGGCAGAGAGCGAGACGAGATCGGAAGAGCACACGT78880.1319098838764917No Hit
CAACGGAATCCCAAAAGCAGCTGAGATCGGAAGAGCACAC78070.13055533258414942No Hit
TGGAGAGAAAGGCAGTTCCTGAAGATCGGAAGAGCACACG74110.12393308182158722No Hit
CGCGACCTCAGATCAGAAGATCGGAAGAGCACACGTCTGA71790.12005337935463156Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTAGACTGAAGCTCCTTGAGAGATCGGAAGAGCACACGTC71750.1199864879327875No Hit
AATGACACGATCACTCCCGTAGATCGGAAGAGCACACGTC69840.1167924225397335No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTC68890.11520375127093703TruSeq Adapter, Index 1 (100% over 40bp)
AACCCGTAGATCCGAACTTGTGAGATCGGAAGAGCACACG67260.11247792583079146No Hit
TACCACAGGGTAGAACCACGGAAGATCGGAAGAGCACACG64990.10868183764114091No Hit
CCCCCCACTGCTAAATTTGACTGGCAGATCGGAAGAGCAC64790.10834738053192058No Hit
AAGCTGCCAGTTGAAGAACTGTAGATCGGAAGAGCACACG64210.10737745491518166No Hit
CAACGGAATCCCACAAGCAGCTAGATCGGAAGAGCACACG63790.10667509498581901No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACCAGATCGGA62330.10423355808851073No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTTCT1250.034.0047262
AACCGTG252.0274826E-434.0047262
GCGCGGT252.0274826E-434.0047262
CAGCATT3750.034.0047263
CGGTTCT200.00278801934.0047263
GGTCCCA200.00278801934.0047263
TAAACGT351.0911808E-634.0047263
GCAAACA301.4833135E-534.0047262
TAAACAC351.0911808E-634.0047263
ATCCGTT200.00278801934.0047262
ATGTAGT408.065581E-834.0047263
AAACGTG408.065581E-834.0047263
AGAGTCT200.00278801934.0047262
TCAATTA1200.034.00472627
AGTGCAT2600.034.0047263
GAGGTAC200.00278801934.0047262
CATGTTC200.00278801934.0047262
CCGGTTC252.0274826E-434.0047262
ACCAACC700.034.0047262
GCAGCAT3750.034.0047262
Produced by FastQC (version 0.11.5)