FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005488325

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005488325
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4025965
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCGTTACCATTACTGAGTAGATCGGAAGAGCACACGTCTGAACTCCA96140723.88016289262326Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCCTGTACTGAGCTGCCCCGAGAGATCGGAAGAGCACACGTCTGAACTCC1628244.044347131681473Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAACCGTTACCATTACTGAGCAGATCGGAAGAGCACACGTCTGAACTCCA1361013.3805808048505144Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCCTGTACTGAGCTGCCCCGAAGATCGGAAGAGCACACGTCTGAACTCCA1007432.5023317390985764Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCCTGTACTGAGCTGCCCCGAGAAGATCGGAAGAGCACACGTCTGAACTC752851.8699864504534938Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCCTGTACTGAGCTGCCCCGAGTAGATCGGAAGAGCACACGTCTGAACTC730561.8146208424564048Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TATTGCACTTGTCCCGGCCTGTAGATCGGAAGAGCACACGTCTGAACTCC670521.6654888952089748Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CAACGGAATCCCAAAAGCAGCTAGATCGGAAGAGCACACGTCTGAACTCC664341.6501385382138196Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCTGTACTGAGCTGCCCCGAGATCGGAAGAGCACACGTCTGAACTCCAG513371.2751476975085476Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TATTGCACTTGTCCCGGCCTGTAAGATCGGAAGAGCACACGTCTGAACTC491791.221545641852326Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACG448501.1140186265901468No Hit
AAACCGTTACCATTACTGAGATCGGAAGAGCACACGTCTGAACTCCAGTC327570.8136434370393184Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATG278930.6928276823072232TruSeq Adapter, Index 10 (100% over 49bp)
AAACCGTTACCATTACTGAGAGATCGGAAGAGCACACGTCTGAACTCCAG270850.6727579598928456Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TGACATGGGACTGCCTAAGCTAAGATCGGAAGAGCACACGTCTGAACTCC260520.6470995152714939Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTGAATTGAAGTGCTTGAATTAGATCGGAAGAGCACACGTCTGAACTCCA218680.5431741209871421Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCCTGTACTGAGCTGCCCCGAAAGATCGGAAGAGCACACGTCTGAACTCC209110.5194034225334795Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GATGGATATGTCTTCAAGGACAGATCGGAAGAGCACACGTCTGAACTCCA205930.511504695147623Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGCCGTC195890.48656657472183684TruSeq Adapter, Index 10 (100% over 50bp)
CGCGACCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTA192910.47916462264326687TruSeq Adapter, Index 10 (100% over 40bp)
TCGCTCTGATACCAAATTGATGAGATCGGAAGAGCACACGTCTGAACTCC183530.4558658607315265Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TATTGCACTTGTCCCGGCCTGTTAGATCGGAAGAGCACACGTCTGAACTC174560.4335854881997236Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCCTGTACTGAGCTGCCCCAAGATCGGAAGAGCACACGTCTGAACTCCAG168270.4179619047855607Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TAACCGTTACCATTACTGAGTAGATCGGAAGAGCACACGTCTGAACTCCA157080.3901673263428768Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AAATCGTTACCATTACTGAGTAGATCGGAAGAGCACACGTCTGAACTCCA149300.370842766889429Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGAGGGGCAGAGAGCGAGACTAGATCGGAAGAGCACACGTCTGAACTCCA140290.34846303929616873Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AAACCGTTACCATTACTGAGAAGATCGGAAGAGCACACGTCTGAACTCCA136650.339421728703553Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AAGCTGCCAGTTGAAGAACTGTAGATCGGAAGAGCACACGTCTGAACTCC135350.3361926892061903Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGATCTCTTCGTACTCTTCTTGAGATCGGAAGAGCACACGTCTGAACTCC129690.3221339480099802Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CAACGGAATCCCAAAAGCAGCCAGATCGGAAGAGCACACGTCTGAACTCC128560.3193271675238111Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TATTGCACTTGTCCCGGCCTGCAGATCGGAAGAGCACACGTCTGAACTCC123900.3077523028640338Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AATGACACGATCACTCCCGTAGATCGGAAGAGCACACGTCTGAACTCCAG117530.2919300093269564Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CATTGCACTTGTCTCGGTCTGAAGATCGGAAGAGCACACGTCTGAACTCC116480.28932193896370184Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAACCGTTACCATTACTGAGTTAGATCGGAAGAGCACACGTCTGAACTCC115090.2858693505780602Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCTGTACTGAGCTGCCCCGAGCAGATCGGAAGAGCACACGTCTGAACTC111710.277473847884917Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GAACCGTTACCATTACTGAGTAGATCGGAAGAGCACACGTCTGAACTCCA97950.2432957067436006Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGGAGAGAAAGGCAGTTCCTGAAGATCGGAAGAGCACACGTCTGAACTCC92820.2305534201116999Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CCCATAAAGTAGAAAGCACTAGATCGGAAGAGCACACGTCTGAACTCCAG83830.20822337004916835Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TGTTTGTTGTACTCGGTCTAGTAGATCGGAAGAGCACACGTCTGAACTCC81790.2031562619148453Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CCCCCCACTGCTAAATTTGACTGGCTTAGATCGGAAGAGCACACGTCTGA81620.20273400290365165Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGACCGTTACCATTACTGAGTAGATCGGAAGAGCACACGTCTGAACTCCA81010.2012188382164276Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AAACCGTTACCATTACTGAGGAGATCGGAAGAGCACACGTCTGAACTCCA80450.1998278673560252Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAGATCGGAAGAGCACACGT77260.1919043012048043No Hit
TGAGGTAGTAGTTTGTGCTGTTAGATCGGAAGAGCACACGTCTGAACTCC72710.1806026629640347Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACCAGATCGGAAGAGCACACG66610.16545101609179413No Hit
TGTAAACATCCTACACTCTCAGCTAGATCGGAAGAGCACACGTCTGAACT65800.1634390760972835Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TAATTTGGTGTTTCTTCGATCAGATCGGAAGAGCACACGTCTGAACTCCA65170.16187423387933078Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CAACGGAATCCCAAAAGCAGCAAGATCGGAAGAGCACACGTCTGAACTCC61650.15313098847108705Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAACCGTTACCATTACTAAGATCGGAAGAGCACACGTCTGAACTCCAGTC61440.15260937439843617Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CAACGGAATCCCAAAAGCAGCTGAGATCGGAAGAGCACACGTCTGAACTC60180.14947968996253072Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCTAGCAGCTGTTGAGCAGGTAGATCGGAAGAGCACACGTCTGAACTCCA59770.14846130058259324Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCCTGTACTGAGCTGCCCCTAGATCGGAAGAGCACACGTCTGAACTCCAG58130.14438774306284333Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TATTGCACTTGTCCCGGCCTGAGATCGGAAGAGCACACGTCTGAACTCCA57800.14356806380582046Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AAGCTGCCAGTTGAAGAACTAGATCGGAAGAGCACACGTCTGAACTCCAG57610.14309612726389823Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CCCCCCACTGCTAAATTTGACTGGCAGATCGGAAGAGCACACGTCTGAAC57220.1421274154146894Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TATTGCACTTGTCCCGGCCTGAAGATCGGAAGAGCACACGTCTGAACTCC52960.13154610136948533Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCGACCTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTA52810.13117351988902037TruSeq Adapter, Index 10 (100% over 40bp)
CAACCGTTACCATTACTGAGTAGATCGGAAGAGCACACGTCTGAACTCCA52780.1310990035929274Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGGAGAGAAAGGCAGTTCCTGAGATCGGAAGAGCACACGTCTGAACTCCA49300.12245511324614099Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGACATGGGACTGCCTAAGCTAGATCGGAAGAGCACACGTCTGAACTCCA48940.12156091769302516Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TTATGTCTTGTTGATCTCAATAGATCGGAAGAGCACACGTCTGAACTCCA48600.12071639967063798Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CATTGCACTTGTCTCGGTCTGAGATCGGAAGAGCACACGTCTGAACTCCA47180.11718929498890328Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CAACGGAATCCCAAAAGCAGCAGATCGGAAGAGCACACGTCTGAACTCCA46580.11569896906704355Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AAAGCGTTACCATTACTGAGTAGATCGGAAGAGCACACGTCTGAACTCCA46070.11443219203346278Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCAGGCTCAGTCCCCTCCCGATAGATCGGAAGAGCACACGTCTGAACTCC44810.11130250759755736Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCGACCTCAGATCAGACGTAGATCGGAAGAGCACACGTCTGAACTCCAG43540.10814798439628759Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TGAGGGGCAGAGAGCGAGACTTTAGATCGGAAGAGCACACGTCTGAACTC43450.10792443550800865Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGTGTTCTCAGGTCACCCCTTAGATCGGAAGAGCACACGTCTGAACTCCA43000.10680669106661385Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCTCTCGGCTCCTCGCGGCTCGAGATCGGAAGAGCACACGTCTGAACTCC42540.10566410785985472Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTTCTCACTACTGCACTTGACTAGTAGATCGGAAGAGCACACGTCTGAAC42370.10524184884866113Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCCTGTACTGAGCTGCCCCGAGAGATCGGAAGAGCACACGTCTGAACTCC41080.1020376481166627Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGATTGGAAATTTCGTTGACTAGATCGGAAGAGCACACGTCTGAACTCCA41030.10191345428984108Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AAACCGTTACCATTACTGGGTAGATCGGAAGAGCACACGTCTGAACTCCA40360.10024925701043104Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCATACT502.5465852E-1144.30494310
ATCCAGG8300.044.3049439
AGCAGTG2000.044.30494311
GCTCCAT254.2672527E-544.30494310
CCATTGC3400.044.30494310
GTTGATT207.5960206E-444.3049411
TATTTGC750.044.304949
AAGTATT302.4119E-644.3049411
TCGTTCG207.5960206E-444.3049410
TCCATGC302.4119E-644.304949
GATGTTT551.8189894E-1244.3049411
GAGCTAC700.044.3049410
ACCATTG3450.044.304949
TAGACCC207.5960206E-444.3049410
AGCTTCC4750.044.3049411
CAATTAC800.044.3049410
CGATTAC600.044.3049410
CGTTCGG207.5960206E-444.3049411
TCCTACA20750.044.304949
CATTACG1300.044.3049411