FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005488330

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005488330
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9187108
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCGTTACCATTACTGAGTAGATCGGAAGAGCACACGTCTGAACTCCA255056527.76243623129281Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCCTGTACTGAGCTGCCCCGAGAGATCGGAAGAGCACACGTCTGAACTCC5557616.049357425644718Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCTGTACTGAGCTGCCCCGAAGATCGGAAGAGCACACGTCTGAACTCCA3607653.926861423638429Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AAACCGTTACCATTACTGAGCAGATCGGAAGAGCACACGTCTGAACTCCA3569633.8854773449925704Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCCTGTACTGAGCTGCCCCGAGAAGATCGGAAGAGCACACGTCTGAACTC2343202.550530591346047Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCCTGTACTGAGCTGCCCCGAGATCGGAAGAGCACACGTCTGAACTCCAG2064682.2473666359424533Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TATTGCACTTGTCCCGGCCTGTAGATCGGAAGAGCACACGTCTGAACTCC1881922.0484356992428956Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCTGTACTGAGCTGCCCCGAGTAGATCGGAAGAGCACACGTCTGAACTC1679161.8277351262225283Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TATTGCACTTGTCCCGGCCTGTAAGATCGGAAGAGCACACGTCTGAACTC1396341.5198906990099605Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CAACGGAATCCCAAAAGCAGCTAGATCGGAAGAGCACACGTCTGAACTCC1071351.1661449936149657Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAACCGTTACCATTACTGAGATCGGAAGAGCACACGTCTGAACTCCAGTC846200.9210733127334522Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TCCTGTACTGAGCTGCCCCGAAAGATCGGAAGAGCACACGTCTGAACTCC688690.7496265418889165Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAACCGTTACCATTACTGAGAGATCGGAAGAGCACACGTCTGAACTCCAG681760.742083362903756Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCCTGTACTGAGCTGCCCCAAGATCGGAAGAGCACACGTCTGAACTCCAG626160.6815637739319054Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TAACCGTTACCATTACTGAGTAGATCGGAAGAGCACACGTCTGAACTCCA435970.47454541733916705Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CATTGCACTTGTCTCGGTCTGAAGATCGGAAGAGCACACGTCTGAACTCC426280.4639980285417348Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACG421740.459056321096911No Hit
TATTGCACTTGTCCCGGCCTGTTAGATCGGAAGAGCACACGTCTGAACTC365860.3982319572165691Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AAACCGTTACCATTACTGAGAAGATCGGAAGAGCACACGTCTGAACTCCA351870.3830040966101629Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AAATCGTTACCATTACTGAGTAGATCGGAAGAGCACACGTCTGAACTCCA351480.38257958870190706Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TATTGCACTTGTCCCGGCCTGCAGATCGGAAGAGCACACGTCTGAACTCC326790.3557049726638677Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGACATGGGACTGCCTAAGCTAAGATCGGAAGAGCACACGTCTGAACTCC290140.31581211410598414Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AATGACACGATCACTCCCGTAGATCGGAAGAGCACACGTCTGAACTCCAG280990.3058525054892138Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCATCTCGTATG267270.29091853497313847TruSeq Adapter, Index 14 (97% over 49bp)
GAACCGTTACCATTACTGAGTAGATCGGAAGAGCACACGTCTGAACTCCA261920.2850951572573219Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCCTGTACTGAGCTGCCCCGAGCAGATCGGAAGAGCACACGTCTGAACTC251340.27357901964361364Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGTAAACATCCTACACTCTCAGCTAGATCGGAAGAGCACACGTCTGAACT241240.2625853533016048Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GATGGATATGTCTTCAAGGACAGATCGGAAGAGCACACGTCTGAACTCCA221650.24126199452537186Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AAACCGTTACCATTACTGAGTTAGATCGGAAGAGCACACGTCTGAACTCC217720.23698426098833278Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGAGGGGCAGAGAGCGAGACTAGATCGGAAGAGCACACGTCTGAACTCCA215810.23490526071969545Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGACCGTTACCATTACTGAGTAGATCGGAAGAGCACACGTCTGAACTCCA209730.22828729127816935Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AAACCGTTACCATTACTGAGGAGATCGGAAGAGCACACGTCTGAACTCCA204640.22274691883452333Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TTGAATTGAAGTGCTTGAATTAGATCGGAAGAGCACACGTCTGAACTCCA202590.22051553111164032Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCGCTCTGATACCAAATTGATGAGATCGGAAGAGCACACGTCTGAACTCC202010.21988421165833688Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CAACGGAATCCCAAAAGCAGCCAGATCGGAAGAGCACACGTCTGAACTCC200240.21795759884394522Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CCCATAAAGTAGAAAGCACTAGATCGGAAGAGCACACGTCTGAACTCCAG193220.21031645649534106Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCCTGTACTGAGCTGCCCCTAGATCGGAAGAGCACACGTCTGAACTCCAG192830.2098919485870853Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCAGGCTCAGTCCCCTCCCGATAGATCGGAAGAGCACACGTCTGAACTCC189250.2059951836856604Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TATTGCACTTGTCCCGGCCTGAGATCGGAAGAGCACACGTCTGAACTCCA186370.20286035605546382Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CCCCCCACTGCTAAATTTGACTGGCTTAGATCGGAAGAGCACACGTCTGA184150.20044392642385397Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCTCTCGGCTCCTCGCGGCTCGAGATCGGAAGAGCACACGTCTGAACTCC181530.19759210406582792Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAGCTGCCAGTTGAAGAACTGTAGATCGGAAGAGCACACGTCTGAACTCC175320.19083263198821654Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CATTGCACTTGTCTCGGTCTGAGATCGGAAGAGCACACGTCTGAACTCCA174660.19011423398962982Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AAAGCGTTACCATTACTGAGTAGATCGGAAGAGCACACGTCTGAACTCCA162870.1772810333785126Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCATCTCGTATGCCGTC162040.176377593471199TruSeq Adapter, Index 14 (98% over 50bp)
TGATCTCTTCGTACTCTTCTTGAGATCGGAAGAGCACACGTCTGAACTCC161330.17560477138181024Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TATTGCACTTGTCCCGGCCTGAAGATCGGAAGAGCACACGTCTGAACTCC158890.17294887575067147Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAACCGTTACCATTACTAAGATCGGAAGAGCACACGTCTGAACTCCAGTC153520.1671037283985341Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TGGAGAGAAAGGCAGTTCCTGAAGATCGGAAGAGCACACGTCTGAACTCC152700.16621117330938093Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CAAAGAATTCTCCTTTTGGGAGATCGGAAGAGCACACGTCTGAACTCCAG148950.16212936649922913Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GCCTGTACTGAGCTGCCCCGAGAGATCGGAAGAGCACACGTCTGAACTCC136760.14886077316169571Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGCTCGGTCTGAGGCCCCTCAAGATCGGAAGAGCACACGTCTGAACTCCA136320.14838184116263792Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGAGGTAGTAGTTTGTGCTGTTAGATCGGAAGAGCACACGTCTGAACTCC128250.13959779290719126Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCAGGCTCAGTCCCCTCCCGAAGATCGGAAGAGCACACGTCTGAACTCCA122080.1328818601022215Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CCCCCCACTGCTAAATTTGACTGGCAGATCGGAAGAGCACACGTCTGAAC117760.12817961865692662Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AAGCCGTTACCATTACTGAGTAGATCGGAAGAGCACACGTCTGAACTCCA115810.1260570791156477Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TAATCGTTACCATTACTGAGTAGATCGGAAGAGCACACGTCTGAACTCCA108650.11826354931279788Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAGATCGGAAGAGCACACGT107430.1169356014972285No Hit
TCCGGTACTGAGCTGCCCCGAGAGATCGGAAGAGCACACGTCTGAACTCC106890.11634782131656665Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGTAAACATCCTACACTCTCAGCAGATCGGAAGAGCACACGTCTGAACTC105570.11491102531939322Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCCTGTACTGAGCTGCCCCGTAGATCGGAAGAGCACACGTCTGAACTCCA101720.11072037032763737Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCCTGTACTGAGCTGCCCCGATAGATCGGAAGAGCACACGTCTGAACTCC101620.11061152214603333Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAGCTGCCAGTTGAAGAACTAGATCGGAAGAGCACACGTCTGAACTCCAG99280.10806447469649862Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TGTTTGTTGTACTCGGTCTAGTAGATCGGAAGAGCACACGTCTGAACTCC97360.10597458960970091Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCGACCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCA96290.10480991406653759TruSeq Adapter, Index 14 (97% over 39bp)
AGCTCGGTCTGAGGCCCCTCAGTAGATCGGAAGAGCACACGTCTGAACTC95180.10360169925073266Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AAACCGTTACCATTACTGGGTAGATCGGAAGAGCACACGTCTGAACTCCA94300.10264383525261703Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CAACGGAATCCCAAAAGCAGCTGAGATCGGAAGAGCACACGTCTGAACTC93570.10184924352690748Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCCTGTACTGAGCTGCCCCCAGATCGGAAGAGCACACGTCTGAACTCCAG93500.10177304979978466Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)