FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005488334

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005488334
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5053012
Sequences flagged as poor quality0
Sequence length40
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTGTACTGAGCTGCCCCGAGAGATCGGAAGAGCACACG53182210.52485131640297No Hit
TCCTGTACTGAGCTGCCCCGAAGATCGGAAGAGCACACGT3515756.957731349143838No Hit
TATTGCACTTGTCCCGGCCTGTAAGATCGGAAGAGCACAC2667745.279504580634283No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGA2445694.840063708536611No Hit
TCCTGTACTGAGCTGCCCCGAGAAGATCGGAAGAGCACAC1987883.9340496321797773No Hit
TCCTGTACTGAGCTGCCCCGAGATCGGAAGAGCACACGTC1813173.588295456254606No Hit
TATTGCACTTGTCCCGGCCTGTAGATCGGAAGAGCACACG1355342.682241799544509No Hit
TCCTGTACTGAGCTGCCCCGAGTAGATCGGAAGAGCACAC1328212.6285510503438343No Hit
AAACCGTTACCATTACTGAGTAGATCGGAAGAGCACACGT1266302.5060300668195525No Hit
TCCTGTACTGAGCTGCCCCGAAAGATCGGAAGAGCACACG1141772.259582997230167No Hit
TCCTGTACTGAGCTGCCCCAAGATCGGAAGAGCACACGTC649681.2857281953812896No Hit
CATTGCACTTGTCTCGGTCTGAAGATCGGAAGAGCACACG504400.9982165092819887No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACCAGATCGGA477780.9455350590895094No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAAGATCGGA437120.8650682009067068No Hit
CTAGACTGAAGCTCCTTGAGGAGATCGGAAGAGCACACGT389830.7714804556173624No Hit
TCCTGTACTGAGCTGCCCCGAGCAGATCGGAAGAGCACAC276700.5475941873876413No Hit
TCCTGTACTGAGCTGCCCCTAGATCGGAAGAGCACACGTC274670.5435767815314905No Hit
TCGCTCTGATACCAAATTGATGAGATCGGAAGAGCACACG268710.531781836259245No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAGATCGGAA266400.5272103054574183No Hit
AAACCGTTACCATTACTGAGCAGATCGGAAGAGCACACGT260310.515158087888966No Hit
TATTGCACTTGTCCCGGCCTGCAGATCGGAAGAGCACACG252620.4999394420595083No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAAT245820.4864821219502349TruSeq Adapter, Index 6 (100% over 39bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTAGATCGG237980.47096662347130785No Hit
TTGAATTGAAGTGCTTGAATTAGATCGGAAGAGCACACGT228490.45218574584821886No Hit
TATTGCACTTGTCCCGGCCTGTTAGATCGGAAGAGCACAC220600.4365712964861354No Hit
TGAGGGGCAGAGAGCGAGACTAGATCGGAAGAGCACACGT219530.43445374758658795No Hit
GATGGATATGTCTTCAAGGACAGATCGGAAGAGCACACGT219330.43405794405396225No Hit
AGCTCGGTCTGAGGCCCCTCAAGATCGGAAGAGCACACGT213480.4224806907246609No Hit
TGACATGGGACTGCCTAAGCTAAGATCGGAAGAGCACACG195700.3872937566742371No Hit
TGAGGTAGTAGTTTGTGCTGTTAGATCGGAAGAGCACACG193200.38234621251641593No Hit
CGCGACCTCAGATCGGAAGAGCACACGTCTGAACTCCAGT176500.3492966175421709Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TATTGCACTTGTCCCGGCCTGAAGATCGGAAGAGCACACG171080.3385703418080147No Hit
TCAGTGCACTACAGAACTTTGTAGATCGGAAGAGCACACG158780.3142284245515348No Hit
TGACTGTGCTGAGTCTGTTCAATCCAACCCTGAGCAGATC154420.3055999075402948No Hit
TCTCTCGGCTCCTCGCGGCTCGAGATCGGAAGAGCACACG151220.29926705101828377No Hit
TTTGGCAATGGTAGAACTCACACTAGATCGGAAGAGCACA150650.29813901095030054No Hit
TCCTGTACTGAGCTGCCCCGATAGATCGGAAGAGCACACG149910.2966745378795855No Hit
TAAGATCCGGACTACAACAAAGAGATCGGAAGAGCACACG137040.27120458055512237No Hit
TCCTGTACTGAGCTGCCCCGTAGATCGGAAGAGCACACGT135110.2673850764652845No Hit
TGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGA128220.25374964476632944No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTATTAGAT125970.24929685502429044No Hit
CAACGGAATCCCAAAAGCAGCTAGATCGGAAGAGCACACG125670.24870314972535193No Hit
AAGGGCTTCCTCTCTGCAGGAAGATCGGAAGAGCACACGT124210.24581378393718442No Hit
AGCTCGGTCTGAGGCCCCTCAGATCGGAAGAGCACACGTC121270.23999547200758675No Hit
CTAGACTGAAGCTCCTTGAGAAGATCGGAAGAGCACACGT111440.22054172837903413No Hit
CGCGACCTCAGATCAGACGTAGATCGGAAGAGCACACGTC99840.19758512348674412No Hit
CATTGCACTTGTCTCGGTCTGAGATCGGAAGAGCACACGT99720.19734764136716873No Hit
CGCGACCTTAGATCGGAAGAGCACACGTCTGAACTCCAGT99040.19600190935624137Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TGTTTGTTGTACTCGGTCTAGTAGATCGGAAGAGCACACG97320.19259799897566046No Hit
TGATCTCTTCGTACTCTTCTTGAGATCGGAAGAGCACACG89690.17749809420599041No Hit
CTAGACTGAAGCTCCTTGAGGAAGATCGGAAGAGCACACG89600.17731998261630885No Hit
TGAGGGGCAGAGAGCGAGACTTTAGATCGGAAGAGCACAC88830.17579613901569996No Hit
TCCTGTACTGAGCTGCCCCCAGATCGGAAGAGCACACGTC84750.16772174695013586No Hit
ATTGCACTTGTCCCGGCCTGTAGATCGGAAGAGCACACGT84540.1673061532408789No Hit
AAACCGTTACCATTACTGAGATCGGAAGAGCACACGTCTG82430.16313042597167787Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GATGGATATGTCTTCAAGGATAGATCGGAAGAGCACACGT81100.16049833247971704No Hit
TGACATGGGACTGCCTAAGCTAGATCGGAAGAGCACACGT77380.15313638677287922No Hit
TATTGCACTTGTCCCGGCCTGAGATCGGAAGAGCACACGT75550.14951478444935418No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATAGATCGGAA74080.14660562848455536No Hit
AAACCGTTACCATTACTGAGAAGATCGGAAGAGCACACGT73070.14460682064479563No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAATAGATCGGAA72870.1442110171121699No Hit
CGCGACCTCAGATCAGACGTGGCAGATCGGAAGAGCACAC70700.1399165487831812No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTATTAAGA70160.13884787924509184No Hit
TCCTGTACTGAGCTGCCCCGAGGAGATCGGAAGAGCACAC67690.13395970561716458No Hit
AGCTCGGTCTGAGGCCCCTCAGTAGATCGGAAGAGCACAC67220.1330295673154942No Hit
TTTGGCAATGGTAGAACTCACAAGATCGGAAGAGCACACG64990.12861635792671777No Hit
TGACTGTGCTGAGTCTGTTCAATCCAACCCTGAGTAGATC64300.12725083573915916No Hit
TCGCTCTGATACCAAATTGATAAGATCGGAAGAGCACACG63810.1262811170842262No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACGAGATCGGA62190.12307510846995812No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATTAGATCGGA61330.12137315327966766No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTAGATCGGAA60580.11988889003232131No Hit
TGTAAACATCCCCGACTGGAAGCTAGATCGGAAGAGCACA55270.10938030624110925No Hit
TCTCTCGGCTCCTCGCGGCTCAAGATCGGAAGAGCACACG54730.10831163670301991No Hit
CCCCCCACTGCTAAATTTGACTGGCAGATCGGAAGAGCAC54250.10736170822471824No Hit
TCCTGTACTGAGCTGCCCCGGAGATCGGAAGAGCACACGT53730.10633261903989145No Hit
AGGGCTGGGTCGGTCGGGCTGGGGCGAGATCGGAAGAGCA52770.10443276208328815No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGG52690.10427444067023788No Hit
GCGGGTGATGCGAACTGGAGTCTGAGCAGATCGGAAGAGC52510.10391821749087475No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAAACT1450.034.006121
CTATACG2500.034.006121
TCGGGTG850.034.006121
TTTGGCA35700.034.006121
GTAAATG200.002787419134.006121
TATCCGC200.002787419134.006121
AAATCGT504.456524E-1034.0061151
ATTAGCC252.0269511E-434.0061151
GTCTACG2200.034.0061151
CGACATG252.0269511E-434.0061151
ATCACAT252.0269511E-434.0061151
TCATGTA1000.034.0061151
TCGAAAC2150.034.0061151
TACATGA351.0918011E-634.002082
GACATTG351.0918011E-634.002082
CCTATAC1500.034.002082
TATACGA2500.034.002082
CCCATAT1300.034.002082
TAGAGTG252.0283657E-434.0020752
ATTACAC504.456524E-1034.0020752
Produced by FastQC (version 0.11.5)