FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005488335

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005488335
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3205028
Sequences flagged as poor quality0
Sequence length40
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCGTTACCATTACTGAGTAGATCGGAAGAGCACACGT39340812.274713356638383No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGA2594938.096434726935303No Hit
TCCTGTACTGAGCTGCCCCGAAGATCGGAAGAGCACACGT1677135.232809198546783No Hit
TCCTGTACTGAGCTGCCCCGAGAGATCGGAAGAGCACACG1555684.853873351496461No Hit
TCCTGTACTGAGCTGCCCCGAGAAGATCGGAAGAGCACAC886242.76515524981373No Hit
AAACCGTTACCATTACTGAGCAGATCGGAAGAGCACACGT738452.304036033382548No Hit
TCCTGTACTGAGCTGCCCCGAGTAGATCGGAAGAGCACAC712662.223568717652389No Hit
TATTGCACTTGTCCCGGCCTGTAAGATCGGAAGAGCACAC571821.7841341791709777No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACCAGATCGGA511821.5969283263671956No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAAGATCGGA394591.2311592909640727No Hit
TCCTGTACTGAGCTGCCCCGAGATCGGAAGAGCACACGTC380871.1883515526229411No Hit
TCGCTCTGATACCAAATTGATGAGATCGGAAGAGCACACG379841.1851378521498095No Hit
TCCTGTACTGAGCTGCCCCGAAAGATCGGAAGAGCACACG377281.1771504024301815No Hit
TATTGCACTTGTCCCGGCCTGTAGATCGGAAGAGCACACG294410.9185879187326912No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTAGC275660.8600860897315094TruSeq Adapter, Index 22 (97% over 37bp)
AAACCGTTACCATTACTGAGAAGATCGGAAGAGCACACGT244930.7642054921205056No Hit
TTGAATTGAAGTGCTTGAATTAGATCGGAAGAGCACACGT234690.7322556932419936No Hit
GATGGATATGTCTTCAAGGACAGATCGGAAGAGCACACGT230140.7180592494043734No Hit
TGAGGGGCAGAGAGCGAGACTAGATCGGAAGAGCACACGT192410.6003379689662618No Hit
CATTGCACTTGTCTCGGTCTGAAGATCGGAAGAGCACACG169030.5273900883237214No Hit
TGACATGGGACTGCCTAAGCTAAGATCGGAAGAGCACACG160910.5020548962442761No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAGATCGGAA157540.4915401675117971No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATTAGATCGGA148760.4641457110515103No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATAGATCGGAA147920.4615248291122574No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTAGATCGG142950.4460179443050108No Hit
TCCTGTACTGAGCTGCCCCAAGATCGGAAGAGCACACGTC141460.4413689989603835No Hit
TCCTGTACTGAGCTGCCCCGAGCAGATCGGAAGAGCACAC141290.4408385823774394No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTATTAGAT131780.41116645470804003No Hit
TGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGA128730.40165015719051445No Hit
CAACGGAATCCCAAAAGCAGCTAGATCGGAAGAGCACACG120620.3763461660865365No Hit
GATGGATATGTCTTCAAGGATAGATCGGAAGAGCACACGT114760.3580623944627005No Hit
TGAGGTAGTAGTTTGTGCTGTTAGATCGGAAGAGCACACG106860.33341362384353584No Hit
TGATCTCTTCGTACTCTTCTTGAGATCGGAAGAGCACACG98440.3071424025000718No Hit
TCGCTCTGATACCAAATTGATAAGATCGGAAGAGCACACG92220.28773539575941304No Hit
TATTGCACTTGTCCCGGCCTGTTAGATCGGAAGAGCACAC90020.2808711811566077No Hit
CCCCCCACTGCTAAATTTGACTGGCAGATCGGAAGAGCAC88340.27562941727810175No Hit
TGAGGGGCAGAGAGCGAGACTTTAGATCGGAAGAGCACAC88150.27503659874422315No Hit
TGTTTGTTGTACTCGGTCTAGTAGATCGGAAGAGCACACG86590.27016924657132485No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTATTAAGA84270.26293062026291186No Hit
TCTCTCGGCTCCTCGCGGCTCGAGATCGGAAGAGCACACG82260.25665922419398524No Hit
CTAGACTGAAGCTCCTTGAGGAGATCGGAAGAGCACACGT78510.2449588583937488No Hit
TCTAGCAGCTGTTGAGCAGGTAGATCGGAAGAGCACACGT77190.24084032963206564No Hit
TCCTGTACTGAGCTGCCCCGTAGATCGGAAGAGCACACGT75680.23612898233650376No Hit
TTTGGCAATGGTAGAACTCACACTAGATCGGAAGAGCACA72970.22767351798486626No Hit
AAGGGCTTCCTCTCTGCAGGAAGATCGGAAGAGCACACGT71260.22233815117995848No Hit
AAAAGCTGGGTTGAGAGGGCGAAGATCGGAAGAGCACACG70090.21868763705028474No Hit
TCGCTCTGATACCCAATTGATGAGATCGGAAGAGCACACG65080.20305594834116894No Hit
CCCCCCACTGCTAAATTTGACTGGTAGATCGGAAGAGCAC64670.20177670834700978No Hit
AAACCGTTACCATTACTGAGGAGATCGGAAGAGCACACGT63720.19881261567761657No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACGAGATCGGA60270.18804827914139907No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAATAGATCGGAA59790.18655063231896882No Hit
TGACATGGGACTGCCTAAGCTAGATCGGAAGAGCACACGT59430.18542739720214613No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGG56250.17550548700354568No Hit
CGCGACCTCAGATCAGACGTAGATCGGAAGAGCACACGTC56160.17522467822434No Hit
AAACCGTTACCATTACTGAGATCGGAAGAGCACACGTCTG55440.17297820799069463Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGTAAACATCCCCGACTGGAAGCTAGATCGGAAGAGCACA55020.17166776702106815No Hit
TATTGCACTTGTCCCGGCCTGAAGATCGGAAGAGCACACG55000.17160536507013358No Hit
TATTGCACTTGTCCCGGCCTGCAGATCGGAAGAGCACACG53070.16558357680494523No Hit
TAAGATCCGGACTACAACAAAGAGATCGGAAGAGCACACG53030.16545877290307603No Hit
TCCTGTACTGAGCTGCCCCTAGATCGGAAGAGCACACGTC51960.16212026852807526No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAAAGATCGGAAG51270.15996740122083175No Hit
AGCTCGGTCTGAGGCCCCTCAAGATCGGAAGAGCACACGT50790.1584697543984015No Hit
TGACTGTGCTGAGTCTGTTCAATCCAACCCTGAGCAGATC50290.15690970562503664No Hit
CTTCTCACTACTGCACTTGACTAGTAGATCGGAAGAGCAC49600.15475683831779316No Hit
AGATCGGAAGAGCCCACGTCTGAACTCCAGTCACGGTAGC49550.15460083344045666TruSeq Adapter, Index 11 (97% over 35bp)
CCCCCCACTGCTAAATTTGACTGGCTTAGATCGGAAGAGC49270.15372720612737237No Hit
TCCTGTACTGAGCTGCCCCGATAGATCGGAAGAGCACACG45160.1409036052103133No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAAGATCGGAAG45030.14049799252923845No Hit
TTGAATTGAAGTGCTTGAATCAGATCGGAAGAGCACACGT44170.13781470863905088No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGG42780.1334777730490966No Hit
TGAGGGGCAGAGAGCGAGACCAGATCGGAAGAGCACACGT38000.11856370677572864No Hit
CATTGCACTTGTCTCGGTCTGAGATCGGAAGAGCACACGT36600.11419557021030706No Hit
TCAGTGCACTACAGAACTTTGTAGATCGGAAGAGCACACG34820.10864179657712819No Hit
CTTCTCACTACTGCACTTGACTAGTCAGATCGGAAGAGCA34620.10801777706778225No Hit
TCCTGTACTGAGCTGCCCCGAGGAGATCGGAAGAGCACAC33610.10486647854558524No Hit
TGAGGGGCAGAGCGCGAGACTAGATCGGAAGAGCACACGT32920.10271361123834176No Hit
TCTCTCGGCTCCTCGCGGCTCAAGATCGGAAGAGCACACG32410.10112236148950961No Hit
CGCGACCTCAGATCAGACGTGGCAGATCGGAAGAGCACAC32260.10065434685750015No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCAAC455.982656E-934.005791
CGAAACT850.034.005791
CATGCCT1150.034.005791
TTCAAGT3100.034.005791
TTTCCGG2100.034.005791
TCCTATA553.274181E-1134.005791
TGTGCGC200.002787399334.005791
GCGCAGT1450.034.005791
TAACCGT950.034.005791
ATATATG200.002787399334.005791
TCATGTA408.061397E-834.005791
AGTCTTG200.002787399334.005791
GATGCCT553.274181E-1134.005791
AAATCGT1400.034.0057871
CTATACG1200.034.0057871
TATGGCA700.034.0057871
TATGGAT1950.034.0057871
CGACATG252.0269008E-434.0057871
ATCACAT351.0907061E-634.0057871
TATTGCG301.4827816E-534.0057871
Produced by FastQC (version 0.11.5)