FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005488336

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005488336
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7742343
Sequences flagged as poor quality0
Sequence length40
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTGTACTGAGCTGCCCCGAGAGATCGGAAGAGCACACG119884815.484304944898463No Hit
TCCTGTACTGAGCTGCCCCGAGAAGATCGGAAGAGCACAC5554177.17375864127952No Hit
TCCTGTACTGAGCTGCCCCGAAGATCGGAAGAGCACACGT5400056.974697452696168No Hit
TCCTGTACTGAGCTGCCCCGAGTAGATCGGAAGAGCACAC4979766.431851443419647No Hit
TCCTGTACTGAGCTGCCCCGAGATCGGAAGAGCACACGTC2783043.59457079078No Hit
TATTGCACTTGTCCCGGCCTGTAGATCGGAAGAGCACACG2697163.483648296129479No Hit
TATTGCACTTGTCCCGGCCTGTAAGATCGGAAGAGCACAC1582652.044148651125376No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGA1435041.8534957699497427No Hit
AAACCGTTACCATTACTGAGTAGATCGGAAGAGCACACGT1249261.6135425671531214No Hit
TCCTGTACTGAGCTGCCCCGAAAGATCGGAAGAGCACACG1204851.5561826697680534No Hit
TCCTGTACTGAGCTGCCCCGAGCAGATCGGAAGAGCACAC715500.9241388556409862No Hit
CAACGGAATCCCAAAAGCAGCTAGATCGGAAGAGCACACG672080.868057640949258No Hit
TCCTGTACTGAGCTGCCCCAAGATCGGAAGAGCACACGTC656140.847469557987808No Hit
TGAGGGGCAGAGAGCGAGACTAGATCGGAAGAGCACACGT559260.7223394778557344No Hit
TATTGCACTTGTCCCGGCCTGCAGATCGGAAGAGCACACG455090.5877936433454317No Hit
TATTGCACTTGTCCCGGCCTGTTAGATCGGAAGAGCACAC416640.5381316740940049No Hit
CATTGCACTTGTCTCGGTCTGAAGATCGGAAGAGCACACG414760.5357034685753395No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAGATCGGAA403510.5211729834237516No Hit
TGTAAACATCCCCGACTGGAAGCTAGATCGGAAGAGCACA399610.5161357485712013No Hit
TTGAATTGAAGTGCTTGAATTAGATCGGAAGAGCACACGT366540.47342258021893374No Hit
TCTCTCGGCTCCTCGCGGCTCGAGATCGGAAGAGCACACG340030.43918229920839214No Hit
TGACATGGGACTGCCTAAGCTAAGATCGGAAGAGCACACG300780.3884870510128523No Hit
ATTGCACTTGTCCCGGCCTGTAGATCGGAAGAGCACACGT230100.2971968563004765No Hit
TGAGGGGCAGAGAGCGAGACTTTAGATCGGAAGAGCACAC221270.2857920399548302No Hit
TCCTGTACTGAGCTGCCCCTAGATCGGAAGAGCACACGTC214950.27762913629633823No Hit
TGAGGTAGTAGTTTGTGCTGTTAGATCGGAAGAGCACACG212730.27476178722642486No Hit
GGCTGGTCCGATGGTAGTGGGTTATCCGAACTAGATCGGA207980.26862669349575446No Hit
CGCGACCTCAGATCAGAAGATCGGAAGAGCACACGTCTGA205230.2650747971253663Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TATTGCACTTGTCCCGGCCTGAAGATCGGAAGAGCACACG204450.2640673501548562No Hit
CGCGACCTCAGATCAGACGTAGATCGGAAGAGCACACGTC195900.25302418143964944No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACCAGATCGGA191570.24743155915463835No Hit
TATTGCACTTGTCCCGGCCTGAGATCGGAAGAGCACACGT187710.24244598824929353No Hit
TGGAGAGAAAGGCAGTTCCTGAAGATCGGAAGAGCACACG182750.23603965879579347No Hit
CGCGACCTCAGATCGGAAGAGCACACGTCTGAACTCCAGT179790.23221652670257573Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AAACCGTTACCATTACTGAGCAGATCGGAAGAGCACACGT168210.21725981398654126No Hit
CAACGGAATCCCAAAAGCAGCTGAGATCGGAAGAGCACAC165570.21384999347096867No Hit
TCCTGTACTGAGCTGCCCCGATAGATCGGAAGAGCACACG164840.21290712643446566No Hit
TCCTGTACTGAGCTGCCCCGAGGAGATCGGAAGAGCACAC162070.20932939809047468No Hit
AGCTCGGTCTGAGGCCCCTCAGTAGATCGGAAGAGCACAC161730.20889025453922672No Hit
GATGGATATGTCTTCAAGGACAGATCGGAAGAGCACACGT161390.2084511109879787No Hit
TCGCTCTGATACCAAATTGATGAGATCGGAAGAGCACACG151370.19550929221296445No Hit
CAACGGAATCCCACAAGCAGCTAGATCGGAAGAGCACACG138540.17893808114675364No Hit
ACCACAGGGTAGAACCACGGAAGATCGGAAGAGCACACGT137290.1773235827965772No Hit
TGAGGGGCAGAGAGCGAGACTTAGATCGGAAGAGCACACG136930.1768586072717264No Hit
TCCTGTACTGAGCTGCCCCGTAGATCGGAAGAGCACACGT136910.17683277529812358No Hit
AGCTCGGTCTGAGGCCCCTCAAGATCGGAAGAGCACACGT134960.17431415787184837No Hit
CTAGACTGAAGCTCCTTGAGGAGATCGGAAGAGCACACGT132200.1707493455146588No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATAGATCGGAA131160.16940608288731202No Hit
TCCTGTACTGAGCTGCCCCCAGATCGGAAGAGCACACGTC123870.15999032850908312No Hit
AGCTCGGTCTGAGGCCCCTCAGATCGGAAGAGCACACGTC120570.15572805286461733No Hit
AAAAGCTGGGTTGAGAGGGCGAAGATCGGAAGAGCACACG120250.15531474128697217No Hit
TCAGGCTCAGTCCCCTCCCGAAGATCGGAAGAGCACACGT118700.1533127633327534No Hit
CAACGGAATCCCAAAAGCAGCCAGATCGGAAGAGCACACG117650.1519565847186052No Hit
TCAGGCTCAGTCCCCTCCCGATAGATCGGAAGAGCACACG116980.15109121360291064No Hit
AAGGGCTTCCTCTCTGCAGGAAGATCGGAAGAGCACACGT110480.14269582218199323No Hit
TCCTGTACTGAGCGTCGCTAGATCGGAAGAGCACACGTCT106910.13808481489388935Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCCCCCACTGCTAAATTTGACTGGCAGATCGGAAGAGCAC104450.13490748214074214No Hit
CGCGACCTCAGATCAGACGTGGCAGATCGGAAGAGCACAC100330.12958609557856066No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGA99210.12813950505680258TruSeq Adapter, Index 8 (100% over 39bp)
GGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTC99170.12808784110959692TruSeq Adapter, Index 8 (100% over 40bp)
CATTGCACTTGTCTCGGTCTGAGATCGGAAGAGCACACGT95920.12389014539913822No Hit
AAGCTGCCAGTTGAAGAACTGTAGATCGGAAGAGCACACG92460.11942121396584988No Hit
AACCCGTAGATCCGAACTTGTAGATCGGAAGAGCACACGT92450.11940829797904846No Hit
CCCCCCACTGCTAAATTTGACTGGCTTAGATCGGAAGAGC91890.11868500271816944No Hit
TCCGGGTGCCCCCTCCAAGATCGGAAGAGCACACGTCTGA90840.11732882410402123Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCCCGGCATCTCCACCAAGATCGGAAGAGCACACGTCTG90120.11639887305431959Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCTCTCGGCTCCTCGCGGCTCAAGATCGGAAGAGCACACG89900.11611472134468856No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTAGATCGG85850.11088374669011693No Hit
TGATTGGAAATTTCGTTGACTAGATCGGAAGAGCACACGT84380.10898509663030945No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAAAGATCGGAAG84280.10885593676229534No Hit
TGTAAACATCCCCGACTGGAAGAGATCGGAAGAGCACACG82670.10677646288726811No Hit
TGAGGGGCAGAGAGCGAGACCAGATCGGAAGAGCACACGT80180.10356038217371666No Hit
TACCACAGGGTAGAACCACGGAAGATCGGAAGAGCACACG78280.1011063446814485No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACGGG950.034.0035131
CGAAACT2250.034.0035131
TTATGTC10550.034.0035131
CTATACG1050.034.0035131
CTATACA200.002788556534.0035131
GTCTACG1850.034.0035131
CGACATG200.002788556534.0035131
TAACGGA800.034.0035131
TTATAAA252.0279616E-434.0035131
AAACCGC750.034.0035131
TATTACA900.034.0035131
AACGACA200.002788556534.0035131
ATTGGTT351.091541E-634.0035131
TGAGGGT351.091541E-634.0035131
TATAAAC252.0279616E-434.0035131
TATCGCA4000.034.0035131
TAATGCA408.068673E-834.0035131
CATGCTC301.48379695E-534.0032962
CAGCCGC200.002788644734.0032962
TAAACAC504.456524E-1034.0032963
Produced by FastQC (version 0.11.5)