FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005488339

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005488339
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6374846
Sequences flagged as poor quality0
Sequence length40
%GC56

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTGTACTGAGCTGCCCCGAGAGATCGGAAGAGCACACG99392215.591309970468306No Hit
TCCTGTACTGAGCTGCCCCGAAGATCGGAAGAGCACACGT4621407.249430025446888No Hit
TCCTGTACTGAGCTGCCCCGAGAAGATCGGAAGAGCACAC3662855.745785858983887No Hit
TCCTGTACTGAGCTGCCCCGAGATCGGAAGAGCACACGTC2860244.4867593664223415No Hit
TCCTGTACTGAGCTGCCCCGAGTAGATCGGAAGAGCACAC2321423.641531105221993No Hit
AAACCGTTACCATTACTGAGTAGATCGGAAGAGCACACGT1875202.941561254969924No Hit
TATTGCACTTGTCCCGGCCTGTAGATCGGAAGAGCACACG1434452.250172004155081No Hit
TATTGCACTTGTCCCGGCCTGTAAGATCGGAAGAGCACAC1271411.9944168063040268No Hit
TCCTGTACTGAGCTGCCCCGAAAGATCGGAAGAGCACACG1082121.6974841431463599No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGA894221.4027319248182621No Hit
TGAGGGGCAGAGAGCGAGACTAGATCGGAAGAGCACACGT743181.1658007111073743No Hit
TCCTGTACTGAGCTGCCCCAAGATCGGAAGAGCACACGTC595680.934422572717835No Hit
TCCTGTACTGAGCTGCCCCGAGGAGATCGGAAGAGCACAC583100.9146887626775612No Hit
CATTGCACTTGTCTCGGTCTGAAGATCGGAAGAGCACACG539590.8464361335160095No Hit
TCCTGTACTGAGCTGCCCCGGAGATCGGAAGAGCACACGT444990.6980403918777018No Hit
TCTCTCGGCTCCTCGCGGCTCGAGATCGGAAGAGCACACG388150.6088774536671161No Hit
TCCTGTACTGAGCTGCCCCGAGCAGATCGGAAGAGCACAC351700.5516996018413621No Hit
TGAGGTAGTAGTTTGTGCTGTTAGATCGGAAGAGCACACG304480.47762722424980936No Hit
TGTAAACATCCCCGACTGGAAGCTAGATCGGAAGAGCACA287900.45161875282948005No Hit
TTGAATTGAAGTGCTTGAATTAGATCGGAAGAGCACACGT280040.4392890432176715No Hit
CGCGACCTCAGATCGGAAGAGCACACGTCTGAACTCCAGT272920.428120145961173Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CAACGGAATCCCAAAAGCAGCTAGATCGGAAGAGCACACG272100.42683384037826166No Hit
AAACCGTTACCATTACTGAGCAGATCGGAAGAGCACACGT260590.40877850225715257No Hit
TATTGCACTTGTCCCGGCCTGCAGATCGGAAGAGCACACG254690.399523376721571No Hit
TGACATGGGACTGCCTAAGCTAAGATCGGAAGAGCACACG233420.36615786483312696No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAGATCGGAA230770.3620009016688403No Hit
GGCTGGTCCGATGGTCGTGGGTTATCAGAACTAGATCGGA221750.3478515402568156No Hit
TATTGCACTTGTCCCGGCCTGTTAGATCGGAAGAGCACAC220000.34510637590304144No Hit
TCCTGTACTGAGCTGCCCCTAGATCGGAAGAGCACACGTC206390.3237568405574033No Hit
CATTGCACTTGTCTCGGTCTGAGATCGGAAGAGCACACGT181680.2849951198821117No Hit
ATTGCACTTGTCCCGGCCTGTAGATCGGAAGAGCACACGT177070.2777635726415979No Hit
TATTGCACTTGTCCCGGCCTGAAGATCGGAAGAGCACACG174430.27362229613076144No Hit
AAACCGTTACCATTACTGAGGAGATCGGAAGAGCACACGT173460.27210069074609805No Hit
TGAGGGGCAGAGAGCGAGACTTTAGATCGGAAGAGCACAC172650.2708300718166368No Hit
TATTGCACTTGTCCCGGCCTGAGATCGGAAGAGCACACGT158990.24940210320374798No Hit
TATTGCACTTGTCCCGGCCTGGAGATCGGAAGAGCACACG153320.2405077706975196No Hit
TCCTGTACTGAGCTGCCCCGTAGATCGGAAGAGCACACGT144240.2262642893647941No Hit
TGGAGAGAAAGGCAGTTCCTGAAGATCGGAAGAGCACACG141600.22212301285395755No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACCAGATCGGA138780.2176993765810186No Hit
TGAGGGGCAGAGAGCGAGACTTAGATCGGAAGAGCACACG138700.21757388335341749No Hit
TGAGGGGCAGAGCGCGAGACTAGATCGGAAGAGCACACGT137560.2157856048601017No Hit
TCCTGTACTGAGCTGCCCCGATAGATCGGAAGAGCACACG135580.21267964747697432No Hit
TGAGGGGCAGAGAGCGAGACCAGATCGGAAGAGCACACGT121490.19057715276572956No Hit
AGCTCGGTCTGAGGCCCCTCAGTAGATCGGAAGAGCACAC121100.18996537328117416No Hit
AAACCGTTACCCTTACTGAGTAGATCGGAAGAGCACACGT118570.1859966499582892No Hit
CTAGACTGAAGCTCCTTGAGGAGATCGGAAGAGCACACGT116460.18268676608031No Hit
AGCTCGGTCTGAGGCCCCTCAAGATCGGAAGAGCACACGT113440.17794939673836827No Hit
TCCTGTACTGAGCTGCCCCCAGATCGGAAGAGCACACGTC112640.1766944644623572No Hit
AGGGCTGGGTCGGTCGGGCTGGGGCGAGATCGGAAGAGCA110920.17399636006893343No Hit
AAGGGCTTCCTCTCTGCAGGAAGATCGGAAGAGCACACGT107760.16903937757868975No Hit
TCGCTCTGATACCAAATTGATGAGATCGGAAGAGCACACG103730.16271765623828402No Hit
TATTGCACTTGTCCCGGCCTGTGAGATCGGAAGAGCACAC99300.1557684687598728No Hit
AGCTCGGTCTGAGGCCCCTCAGATCGGAAGAGCACACGTC99220.15564297553227166No Hit
GATGGATATGTCTTCAAGGACAGATCGGAAGAGCACACGT99190.15559591557192126No Hit
TCTCTCGGCTCCTCGCGGCTCAAGATCGGAAGAGCACACG98770.15493707612701546No Hit
AAACCGTTACCATTACTGAGATCGGAAGAGCACACGTCTG98250.15412137014760827Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGCGACCTCAGATCAGAAGATCGGAAGAGCACACGTCTGA88680.13910924279582598Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGAGGGGCAGAGAGCGAGACGAGATCGGAAGAGCACACGT87680.13754057745081213No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACGAGATCGGA87080.13659937824380383No Hit
ACCACAGGGTAGAACCACGGAAGATCGGAAGAGCACACGT86540.13575229895749638No Hit
TCCTGTACTGAGCTGCCCCGGGAGATCGGAAGAGCACACG86500.13568955234369584No Hit
TCAGGCTCAGTCCCCTCCCGATAGATCGGAAGAGCACACG86210.1352346393936418No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACG85780.1345601132952859TruSeq Adapter, Index 1 (100% over 39bp)
AAACCGTTACCATTACTGAGTTAGATCGGAAGAGCACACG82260.1290384112808372No Hit
TGTAAACATCCCCGACTGGAAGAGATCGGAAGAGCACACG80880.12687365310471815No Hit
CGCGACCTTAGATCGGAAGAGCACACGTCTGAACTCCAGT79980.1254618542942057Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TCAGGCTCAGTCCCCTCCCGAAGATCGGAAGAGCACACGT79300.1243951618595963No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTC77740.12194804392137472TruSeq Adapter, Index 1 (100% over 40bp)
AAAAGCTGGGTTGAGAGGGCGAAGATCGGAAGAGCACACG75410.11829305366749251No Hit
CGCGACCTCAGATCAGACGTAGATCGGAAGAGCACACGTC75310.11813618713299114No Hit
TCCTGTACTGAGCGTCGCTAGATCGGAAGAGCACACGTCT74770.11728910784668367Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGAGGTAGTAGGTTGTGTGGTTAGATCGGAAGAGCACACG73880.11589299568962137No Hit
AAGCTGCCAGTTGAAGAACTAGATCGGAAGAGCACACGTC72570.11383804408765326No Hit
CAACGGAATCCCACAAGCAGCTAGATCGGAAGAGCACACG68270.1070927831040938No Hit
CATTGCACTTGTCTCGGTCTGGAGATCGGAAGAGCACACG67430.1057751042142822No Hit
AAACCGTTACCATTACTGAGAGATCGGAAGAGCACACGTC66900.10494371158142486No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGCG408.0674E-834.00390634
AAGACCG1100.034.00230823
TTAAAAC252.0283446E-434.00230832
TTAATTA252.0285316E-434.00177428
TAATTAA252.0285316E-434.00177429
GTTTGTT4800.034.0009732
TTCTAAT351.0921394E-634.0009732
AACCGTG252.028812E-434.0009732
CAAGTGT200.002789541634.0009732
GCAAACA200.002789541634.0009732
GTTCGTA553.274181E-1134.0009732
ATGACGC200.002789541634.0009732
CATGTAC1450.034.0009732
CGTGTAC455.99357E-934.0009732
CCGGTTC553.274181E-1134.0009732
CGATGCC200.002789541634.0009732
CGCGTGA408.0735845E-834.0009732
CCTATAC3200.034.0009732
GTAACAG252.028812E-434.0009732
AATCGTT800.034.0009732
Produced by FastQC (version 0.11.5)